Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis
Yansong HOU, Wei LIANG, Liping ZHANG, Shuiping CHENG, Feng HE, Zhenbin WU
Frontiers of Environmental Science & Engineering
2011,
Volume 5,
Issue 1,
Pages 84-91
doi:
10.1007/s11783-010-0283-1
Abstract:
The study of community composition of algae is essential for understanding the structure and dynamics of the aquatic ecosystem and for evaluating the eutrophic level of the water body. A high-performance liquid chromatographic (HPLC) method based on a reverse-phase C nonpolar column was developed for the main algal taxa, which includes cyanophytes, bacillariophytes, euglenophytes, dinophytes, and chlorophytes. Based on the elution order using HPLC, 19 pigments were identified, and they were chlorophyllide , 19′-butanoyloxyfucoxanthin, chlorophyll + , phephorbides , peridinin, methyl-chlorophyllide , fucoxanthin, neoxanthin, violaxanthin, myxoxanthophyll, diadinoxanthin, diatoxanthin, lutein, zeaxanthin, chlorophyll allomer, chlorophyll , chlorophyll allomer, chlorophyll , and β,β-carotene. A comparison study of cell microscopic counts and accessory pigment analysis indicated that HPLC analysis could be a useful tool for monitoring phytoplankton communities and their abundance.
Keywords：
high-performance liquid chromatographic (HPLC)
algae
pigment
chemotaxonomy
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