Resource Type

Journal Article 4

Year

2021 2

2016 1

Keywords

lncRNA 2

Gene Expression Omnibus 1

HAND2 1

RNA immunoprecipitation 1

RNA-seq 1

SPRED1 1

cell proliferation 1

congenital heart disease 1

cryostorage duration 1

erythroid differentiation 1

hematopoietic stem cell 1

human metaphase II oocyte 1

lncRNA LOC646029 1

lncRNA SAP30-2:1 1

metastasis 1

miRNA 1

microRNA 627-3p 1

ovarian cancer 1

single-cell RNA-Seq 1

vitrification 1

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Long noncoding RNA LOC646029 functions as a ceRNA to suppress ovarian cancer progression through the miR-627-3p/SPRED1 axis

Frontiers of Medicine   Pages 924-938 doi: 10.1007/s11684-023-1004-z

Abstract: In the current study, the lncRNA LOC646029 was markedly downregulated in metastatic ovarian tumors compared

Keywords: ovarian cancer     lncRNA LOC646029     metastasis     microRNA 627-3p     SPRED1    

Global transcriptome analysis for identification of interactions between coding and noncoding RNAs during human erythroid differentiation

Nan Ding,Jiafei Xi,Yanming Li,Xiaoyan Xie,Jian Shi,Zhaojun Zhang,Yanhua Li,Fang Fang,Sihan Wang,Wen Yue,Xuetao Pei,Xiangdong Fang

Frontiers of Medicine 2016, Volume 10, Issue 3,   Pages 297-310 doi: 10.1007/s11684-016-0452-0

Abstract: In the present study, we compared the dynamics of coding genes, miRNA, and lncRNA expression profiles

Keywords: erythroid differentiation     hematopoietic stem cell     RNA-seq     miRNA     lncRNA    

Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes

Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao

Frontiers of Medicine 2021, Volume 15, Issue 1,   Pages 144-154 doi: 10.1007/s11684-020-0792-7

Abstract: Oocyte cryopreservation is widely used for clinical and social reasons. Previous studies have demonstrated that conventional slow-freezing cryopreservation procedures, but not storage time, can alter the gene expression profiles of frozen oocytes. Whether vitrification procedures and the related frozen storage durations have any effects on the transcriptomes of human metaphase II oocytes remain unknown. Four women (30–32 years old) who had undergone IVF treatment were recruited for this study. RNA-Seq profiles of 3 fresh oocytes and 13 surviving vitrified-thawed oocytes (3, 3, 4, and 3 oocytes were cryostored for 1, 2, 3, and 12 months) were analyzed at a single-cell resolution. A total of 1987 genes were differentially expressed in the 13 vitrified-thawed oocytes. However, no differentially expressed genes were found between any two groups among the 1-, 2-, 3-, and 12-month storage groups. Further analysis revealed that the aberrant genes in the vitrified oocytes were closely related to oogenesis and development. Our findings indicated that the effects of vitrification on the transcriptomes of mature human oocytes are induced by the procedure itself, suggesting that long-term cryostorage of human oocytes is safe.

Keywords: human metaphase II oocyte     vitrification     cryostorage duration     single-cell RNA-Seq     lncRNA    

Long non-coding RNA SAP30-2:1 is downregulated in congenital heart disease and regulates cell proliferation by targeting HAND2

Jing Ma, Shiyu Chen, Lili Hao, Wei Sheng, Weicheng Chen, Xiaojing Ma, Bowen Zhang, Duan Ma, Guoying Huang

Frontiers of Medicine 2021, Volume 15, Issue 1,   Pages 91-100 doi: 10.1007/s11684-020-0778-5

Abstract: mining, bioinformatics analysis, and analysis of clinical tissue samples and observed that the novel lncRNAKnockdown of lncRNA SAP30-2:1 inhibited the proliferation of human embryonic kidney and AC16 cells andMoreover, lncRNA SAP30-2:1 was associated with HAND2 by RNA immunoprecipitation.Overall, these results suggest that lncRNA SAP30-2:1 may be involved in heart development through affecting

Keywords: congenital heart disease     Gene Expression Omnibus     lncRNA SAP30-2:1     cell proliferation     RNA immunoprecipitation    

Title Author Date Type Operation

Long noncoding RNA LOC646029 functions as a ceRNA to suppress ovarian cancer progression through the miR-627-3p/SPRED1 axis

Journal Article

Global transcriptome analysis for identification of interactions between coding and noncoding RNAs during human erythroid differentiation

Nan Ding,Jiafei Xi,Yanming Li,Xiaoyan Xie,Jian Shi,Zhaojun Zhang,Yanhua Li,Fang Fang,Sihan Wang,Wen Yue,Xuetao Pei,Xiangdong Fang

Journal Article

Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes

Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao

Journal Article

Long non-coding RNA SAP30-2:1 is downregulated in congenital heart disease and regulates cell proliferation by targeting HAND2

Jing Ma, Shiyu Chen, Lili Hao, Wei Sheng, Weicheng Chen, Xiaojing Ma, Bowen Zhang, Duan Ma, Guoying Huang

Journal Article