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Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 3,   Pages 298-306 doi: 10.1007/s11705-022-2212-9

Abstract: CA-CDs has been successfully applied for two-photon imaging of the pH in liver and kidney of diabetic miceImaging results showed that the pH value in kidney of diabetic mice was lower than that of the normalmice, while the pH value in liver of diabetic mice was almost the same as that of the normal mice.

Keywords： carbon dots     two-photon imaging     pH     diabetic mice    

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Inhibitory activity of Bifidobacterium adolescent combined with cisplatin on melanoma in mice and its

HUANG Hongying, LIU Guangchao, QI Yijun, DU Yaowu, CHEN Jugao, MA Yuanfang

Frontiers of Medicine 2008, Volume 2, Issue 2,   Pages 186-190 doi: 10.1007/s11684-008-0035-9

Abstract: inhibitory activity of Bifidobacterium adolescent combined with cisplatin on the growth of melanoma (B16) in miceC57 mice were inoculated with B16 cancer cells to construct mouse model of melanoma and treated withThe ratio of inhibitory activity reached 74.45% when the mice were treated by bifidobacterium combinedthe inhibitory activity on B16 cell proliferation is correlated with the enhanced immune activity in mice
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Particulate matter 2.5 triggers airway inflammation and bronchial hyperresponsiveness in mice by activating

Frontiers of Medicine 2021, Volume 15, Issue 5,   Pages 750-766 doi: 10.1007/s11684-021-0839-4

Abstract: acetylation, airway inflammation, and bronchial hyperresponsiveness were deteriorated in SIRT2 knockout mice

Keywords： particulate matter 2.5     sirtuin 2     p65     airway inflammation     bronchial hyperresponsiveness     triptolide    

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Immunogenicity and protective immunity against otitis media caused by pneumococcus in mice of Hib conjugate

Zeyu Chen,Rong Guo,Jianghong Xu,Chuangjun Qiu

Frontiers of Medicine 2016, Volume 10, Issue 4,   Pages 490-498 doi: 10.1007/s11684-016-0470-y

Abstract: polysaccharide conjugate vaccine with the pneumococcal surface adhesin A (PsaA) protein carrier in young miceA total of 15 young mice aged 3 weeks to 5 weeks were immunized with the conjugate vaccine, and another15 young mice of the same age were immunized with the licensed Hib-tetanus toxoid (TT) vaccine.Furthermore, the third group of 15 young mice was inoculated with phosphate buffer saline as control.The immunized mice were inoculated with pneumococcus in the middle ear.

Keywords： conjugate vaccine     pneumococcal surface adhesin A     Hemophilus influenzae b     immunogenicity     otitis media    

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Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX

Wei Lu,Qingzhang Zhou,Hao Yang,Hao Wang,Yexing Gu,Qi Shen,Jinglun Xue,Xiaoyan Dong,Jinzhong Chen

Frontiers of Medicine 2016, Volume 10, Issue 2,   Pages 212-218 doi: 10.1007/s11684-016-0438-y

Abstract:

Hemophilia B is a hemorrhagic disease caused by the deficiency of clotting factor IX (FIX). Gene therapy might be the ultimate strategy for the disease. However, two main problems that should be solved in gene therapy for hemophilia B are immunity and safety. Self-complementary adeno-associated virus serotype 8 (scAAV8), a non-human primate AAV featuring low immunogenicity and high transfection efficiency in liver cells, might be a potential vector for hemophilia B gene therapy. A strong liver-specific promoter-1 (LP1) was inserted and mutant human FIX Arg338Ala was introduced into plasmid scAAV8-LP1 to develop an optimized AAV8 vector that expresses human clotting factor FIX (hFIX). The efficiency of scAAV8-LP1-hFIX administered through normal systemic injection or hydrodynamic injection was compared. A high expression was achieved using hydrodynamic injection, and the peak hFIX expression levels in the 5×1011 and 1×1011 virus genome (vg) cohorts were 31.94% and 25.02% of normal level, respectively, at 60 days post-injection. From the perspective of long-term (200 days) expression, both injection methods presented promising results with the concentration value maintained above 4% of normal plasma. The results were further verified by enzyme-linked immunosorbent assay and activated partial thromboplastin time. Our study provides a potential gene therapy method for hemophilia B.

Keywords： hemophilia B     AAV8     hFIX     gene therapy    

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Overexpression of netrin-1 improves neurological outcomes in mice following transient middle cerebral

Haiyan LU, Yongting WANG, Falei YUAN, Jianrong LIU, Lili ZENG, Guo-Yuan YANG

Frontiers of Medicine 2011, Volume 5, Issue 1,   Pages 86-93 doi: 10.1007/s11684-011-0118-x

Abstract: The level of NT-1 was increased in the AAV-NT-1-transduced tMCAO mice compared with the control mice.Furthermore, the neurobehavioral outcomes were significantly improved in AAV-NT-1-transduced mice compared

Keywords： adeno-associated virus     angiogenesis     gene transfer     ischemia     middle cerebral artery occlusion     netrin-1    

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Long-term correction of hemorrhagic diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed

Frontiers of Medicine 2022, Volume 16, Issue 4,   Pages 584-595 doi: 10.1007/s11684-021-0844-7

Abstract: (hLC), and rat light chain (rLC) were transfected into cells and hydrodynamically injected into HA micePurified AAV viruses were intravenously injected into HA mice at two doses.Notably, the HA mice undergoing the AAV-delivered hHC+ rLC treatment exhibited a visibly higher activity+ rLC) via an AAV-delivered gene therapy substantially improved the hemorrhagic diathesis of the HA mice

Keywords： hemophilia A     adeno-associated virus (AAV)     human/rat hybrid factor VIII     gene therapy     dual chain strategy    

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Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells

Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 201-213 doi: 10.15302/J-FASE-2014017

Abstract: In the bone marrow and spleen, the developing B cell populations undergo both negative and positive selections to shape their B cell receptor repertoire. To gain insight into the shift of the immunoglobulin heavy (IgH) chain repertoire during B cell development, we undertook large scale Ig μ chain repertoire analysis of pre-B, immature B and spleen B cell populations. We found that the majority of V gene segments, V families, J and D gene segments, were observed to have significantly different usage frequencies when three B cell populations were compared, but the usage profile of the V D, and J genes between different B cell populations showed high correlations. In both productive and nonproductive rearrangements, the length of CDRH3 shortened significantly on average when B cells entered the periphery. However, the CDRH3 length distribution of nonproductive rearrangements did not follow a Gaussian distribution, but decreased successively in the order 3 -2, 3 -1 and 3 , suggesting a direct correlation between mRNA stability and CDRH3 length patterns of nonproductive rearrangements. Further analysis of the individual components comprising CDRH3 of productive rearrangements indicated that the decrease in CDRH3 length was largely due to the reduction of N addition at the 5′ and 3′ junctions. Moreover, with development, the amino acid content of CDRH3 progressed toward fewer positively charged and nonpolar residues but more polar residues. All these data indicated that the expressed Ig μ chain repertoire, especially the repertoire of CDRH3, was fine-tuned when B cells passed through several checkpoints of selection during the process of maturation.

Keywords： repertoire     complementarity determining region 3 of the IgH chain (CDRH3)     immunoglobulin     heavy chain     variable region    

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Dynein axonemal heavy chain 10 deficiency causes primary ciliary dyskinesia in humans and mice

Frontiers of Medicine   Pages 957-971 doi: 10.1007/s11684-023-0988-8

Abstract: Subsequently, animal models of Dnah10-knockin mice harboring missense variants and Dnah10-knockout mice recapitulated the phenotypes of PCD, including chronic respiratory infection, male infertility

Keywords： DNAH10     mice     motile cilia     mutation     primary ciliary dyskinesia    

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Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 242-248 doi: 10.15302/J-FASE-2015059

Abstract: further study the functions and mechanisms of lactoferrin, we modified the lactoferrin promoter of miceSeven mice with lactoferrin promoter mutations were obtained with an efficiency of 24% (7/29) by injectingplasmid pX330, expressing a small guide RNA and human codon-optimized SpCas9, into fertilized eggs of micepronuclear injection of a circular plasmid is a feasible and efficient method for targeted mutagenesis in mice

Keywords： lactoferrin     promoter     CRISPR/Cas9     plasmid pX330    

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Protective effects of nicotine on gamma-aminobutyric acid neurons and dopaminergic neurons in mice with

Lei FU MD , Dezheng GONG BM , Yan PENG , Dongmei WANG BM , Hong XU , Yue LI MD , Dengqin YU BS , Yanhui FENG MD , Shengming YIN PhD , Jin GONG , Yiping SUN PhD ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 330-335 doi: 10.1007/s11684-009-0051-4

Abstract: aimed to investigate the protective effect of nicotine on dopaminergic neurons and its mechanisms in miceC57BL/6J mice were injected with MPTP for 8 days to establish a PD model.The results showed that pretreatment with nicotine could improve the dyskinesia of PD mice markedly.protective effects on the γ-aminobutyric acid neurons and dopaminergic neurons in the MPTP-treated mice

Keywords： Parkinson disease     nicotine     dopaminergic neuron     gamma-aminobutyric acid neuron    

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Ablation of steroid receptor coactivator-3 in mice impairs adipogenesis and enhances energy expenditure

Ling-Yan XU PhD, Xin-Ran MA PhD, Xiao-Ying LI PhD, MD, Shu WANG PhD, Guang NING PhD, MD, Jie-Li LI PhD, Jian-Ming XU PhD,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 229-234 doi: 10.1007/s11684-010-0028-3

Abstract: We observed that SRC-3 mice were lean, with reduced WAT and decreased serum leptin levels, mainly dueIn the BAT, the lipid droplets decreased significantly in SRC-3 mice as demonstrated by histologicalthermogenic marker gene PPAR coactivator 1α and uncoupling protein-1 increased in BAT of SRC-3 mice

Keywords： steroid receptor coactivator-3     white adipose tissue     brown adipose tissue     obesity     adipocytes     energy expenditure    

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Endogenous tissue factor pathway inhibitor in vascular smooth muscle cells inhibits arterial thrombosis

Jichun Yang, Kaiyue Jin, Jiajun Xiao, Jing Ma, Duan Ma

Frontiers of Medicine 2017, Volume 11, Issue 3,   Pages 403-409 doi: 10.1007/s11684-017-0522-y

Abstract: In this TFPIFlox mice crossbred with Sma–Cre mice were utilized to establish TFPIconditional knockout mice and to examine the effects of VSMC-directed TFPI deletion on development,The mice with deleted TFPI in VSMCs (TFPISma) reproduced viable offspring.cells and cells of hematopoietic origin) mice.that of the TFPIFlox mice.

Keywords： arterial thrombosis     conditional knockout mice     tissue factor pathway inhibitor     vascular smooth muscle    

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Construction of a universal recombinant expression vector that regulates the expression of human lysozyme in milk

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 382-389 doi: 10.15302/J-FASE-2018211

Abstract:

The mammary gland provides a novel method for producing recombinant proteins in milk of transgenic animals. A key component in the technology is the construction of an efficient milk expression vector. Here, we established a simple method to construct a milk expression vector, by a combination of homologous recombination and digestion-ligation. Our methodology is expected to have the advantages of both plasmid and bacterial artificial chromosome (BAC) vectors. The BAC of mouse whey acidic protein gene (mWAP) was modified twice by homologous recombination to produce a universal expression vector, and the human lysozyme gene (hLZ) was then inserted into the vector by a digestion-ligation method. The final vector containing the 8.5 kb mWAP 5′ promoter, 4.8 kb hLZ genomic DNA, and 8.0 kb mWAP 3′ genomic DNA was microinjected into pronuclei of fertilized mouse embryos, to successfully generate two transgenic mouse lines that expressed recombinant human lysozyme (rhLZ) in milk. The highest expression level of rhLZ was 0.45 g·L−1, and rhLZ exhibited the same antibacterial activity as native hLZ. Our results have provided a simple approach to construct a universal milk expression vector, and demonstrated that the resulting vector regulates the expression of hLZ in milk.

Keywords： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

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Loss of liver kinase B1 causes planar polarity defects in cochlear hair cells in mice

Yuqin Men,Aizhen Zhang,Liwen Zhang,Yecheng Jin,Zhishuo Wang,Jing Zhao,Xiaolin Yu,Jian Zhang,Jiangang Gao

Frontiers of Medicine 2016, Volume 10, Issue 4,   Pages 481-489 doi: 10.1007/s11684-016-0494-3

Abstract: In this study, LKB1 conditional knockout mice (LKB1Pax2 CKO mice) were generated usingPax2-Cre mice to investigate the function of LKB1 in inner ear hair cells during early embryonic periodLKB1Pax2 CKO mice died perinatally.LKB1 deficiency in LKB1Pax2 CKO mice thus disrupted hair cell planar polarity duringOur results suggest that LKB1 is required in PCP formation in cochlear hair cells in mice.

Keywords： LKB1     stereociliary bundles     kinocilium     planar cell polarity     hearing     mice    

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Title Author Date Type Operation

Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice

Journal Article

Inhibitory activity of Bifidobacterium adolescent combined with cisplatin on melanoma in mice and its

HUANG Hongying, LIU Guangchao, QI Yijun, DU Yaowu, CHEN Jugao, MA Yuanfang

Journal Article

Particulate matter 2.5 triggers airway inflammation and bronchial hyperresponsiveness in mice by activating

Journal Article

Immunogenicity and protective immunity against otitis media caused by pneumococcus in mice of Hib conjugate

Zeyu Chen,Rong Guo,Jianghong Xu,Chuangjun Qiu

Journal Article

Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX

Wei Lu,Qingzhang Zhou,Hao Yang,Hao Wang,Yexing Gu,Qi Shen,Jinglun Xue,Xiaoyan Dong,Jinzhong Chen

Journal Article

Overexpression of netrin-1 improves neurological outcomes in mice following transient middle cerebral

Haiyan LU, Yongting WANG, Falei YUAN, Jianrong LIU, Lili ZENG, Guo-Yuan YANG

Journal Article

Long-term correction of hemorrhagic diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed

Journal Article

Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells

Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO

Journal Article

Dynein axonemal heavy chain 10 deficiency causes primary ciliary dyskinesia in humans and mice

Journal Article

Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Journal Article

Protective effects of nicotine on gamma-aminobutyric acid neurons and dopaminergic neurons in mice with

Lei FU MD , Dezheng GONG BM , Yan PENG , Dongmei WANG BM , Hong XU , Yue LI MD , Dengqin YU BS , Yanhui FENG MD , Shengming YIN PhD , Jin GONG , Yiping SUN PhD ,

Journal Article

Ablation of steroid receptor coactivator-3 in mice impairs adipogenesis and enhances energy expenditure

Ling-Yan XU PhD, Xin-Ran MA PhD, Xiao-Ying LI PhD, MD, Shu WANG PhD, Guang NING PhD, MD, Jie-Li LI PhD, Jian-Ming XU PhD,

Journal Article

Endogenous tissue factor pathway inhibitor in vascular smooth muscle cells inhibits arterial thrombosis

Jichun Yang, Kaiyue Jin, Jiajun Xiao, Jing Ma, Duan Ma

Journal Article

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme in milk

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Journal Article

Loss of liver kinase B1 causes planar polarity defects in cochlear hair cells in mice

Yuqin Men,Aizhen Zhang,Liwen Zhang,Yecheng Jin,Zhishuo Wang,Jing Zhao,Xiaolin Yu,Jian Zhang,Jiangang Gao

Journal Article