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Journal Article 7

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oocyte 3

TLR4 transgenic ewe 1

DNA damage 1

DNA methylation 1

IVF-ET 1

Intracellular damage 1

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NADPH oxidase 1

Oocyte enucleation 1

Optical flow 1

PCOS 1

ROS 1

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cryostorage duration 1

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Effects of DNA damage on oocyte meiotic maturation and early embryonic development

Shen YIN,Junyu MA,Wei SHEN

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 185-190 doi: 10.15302/J-FASE-2014035

Abstract: DNA damage is one of the most common threats to meiotic cells. It has the potential to induce infertility and genetic abnormalities that may be passed to the embryo. Here, we reviewed exogenous factors which could induce DNA damage. Specially, we addressed the different effects of DNA damage on mouse oocytes and embryonic development. Complex DNA damage, double-strand breaks, represents a more difficult repair process and involves various repair pathways. Understanding the mechanisms involved in DNA damage responses may improve therapeutic strategies for ovarian cancer and fertility preservation.

Keywords: DNA damage     double-strand breaks (DSBs)     oocyte     embryo    

Mouse Oocyte Enucleation with Surface Tension Assisted Method

Meng Qinggang,Zhu Shien,Zeng Shenming,Zhang Zhongcheng

Strategic Study of CAE 2001, Volume 3, Issue 11,   Pages 65-69

Abstract:

In the present experiment a new simple method-Surface Tension Aided (STA)- for mouse oocyte enucleationThe manipulation time in method A (3 min/oocyte) was significantly longer than that in method STA (1.33min/oocyte), method B (1.30 min/oocyte) and method C (1.41 min/ oocyte) ; The cytoplasm loss in method C

Keywords: surface tension assisted     enucleation     oocyte     mouse    

Oxidative stress in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with

Qiaohong Lai, Wenpei Xiang, Qing Li, Hanwang Zhang, Yufeng Li, Guijin Zhu, Chengliang Xiong, Lei Jin

Frontiers of Medicine 2018, Volume 12, Issue 5,   Pages 518-524 doi: 10.1007/s11684-017-0575-y

Abstract: increased ROS expression levels in PCOS GCs greatly induced cell apoptosis, which further affected the oocyte

Keywords: PCOS     ROS     granulosa cell     IVF-ET     NADPH oxidase    

Intracellular Strain Evaluation-Based Oocyte Enucleation and Its Application in Robotic Cloning Article

Mingzhu Sun, Yaowei Liu, Maosheng Cui, Qili Zhao, Xiangfei Zhao, Yidi Zhang, Jingjing Huang, Guizhang Lu, Xin Zhao

Engineering 2023, Volume 24, Issue 5,   Pages 73-83 doi: 10.1016/j.eng.2022.04.016

Abstract: In this study, we proposed an intracellular strain evaluation-based oocyte enucleation method to reduce

Keywords: Oocyte enucleation     Robotic cloning     Intracellular strain     Intracellular damage     Optical flow    

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review

Fengxia YIN,Hui LIU,Shorgan BOU,Guangpeng LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 2,   Pages 104-113 doi: 10.15302/J-FASE-2014003

Abstract: The oocyte first obtains its reprogramming capability in the early fetal follicle, and then its capacity

Keywords: nuclear reprogramming     somatic cell     transcription factors     transcriptomics    

Effects of the

Yi FANG,Xiangwei FU,Junjie LI,Ming DU,Baoyu JIA,Jinlong ZHANG,Xiaosheng ZHANG,Shien ZHU

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 4,   Pages 314-320 doi: 10.15302/J-FASE-2014038

Abstract: This study was conducted to systematically assess the reproductive performance of transgenic ewes. In the transgenic founders (F ) and their positive offspring (F ), hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were analyzed. The values of the physiological and biochemical parameters determined from the blood samples did not differ significantly between the transgenic and wild-type ewes. Moreover, the transgenic ewes showed reproductive traits similar to the wild-type ewes. These traits included characteristics of puberty, the estrus cycle, estrus duration, gestation, the pregnancy rate and the superovulation response. Additionally, no significant differences were found between transgenic and wild-type ewes in the DNA methylation level of the oocytes at various stages. In summary, the preliminary evidence presented in this paper demonstrates that the presence of the transgene did not affect the reproductive performance in sheep.

Keywords: TLR4 transgenic ewe     safety assessment     reproductive trait     oocyte     DNA methylation    

Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes

Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao

Frontiers of Medicine 2021, Volume 15, Issue 1,   Pages 144-154 doi: 10.1007/s11684-020-0792-7

Abstract: Oocyte cryopreservation is widely used for clinical and social reasons.

Keywords: human metaphase II oocyte     vitrification     cryostorage duration     single-cell RNA-Seq     lncRNA    

Title Author Date Type Operation

Effects of DNA damage on oocyte meiotic maturation and early embryonic development

Shen YIN,Junyu MA,Wei SHEN

Journal Article

Mouse Oocyte Enucleation with Surface Tension Assisted Method

Meng Qinggang,Zhu Shien,Zeng Shenming,Zhang Zhongcheng

Journal Article

Oxidative stress in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with

Qiaohong Lai, Wenpei Xiang, Qing Li, Hanwang Zhang, Yufeng Li, Guijin Zhu, Chengliang Xiong, Lei Jin

Journal Article

Intracellular Strain Evaluation-Based Oocyte Enucleation and Its Application in Robotic Cloning

Mingzhu Sun, Yaowei Liu, Maosheng Cui, Qili Zhao, Xiangfei Zhao, Yidi Zhang, Jingjing Huang, Guizhang Lu, Xin Zhao

Journal Article

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review

Fengxia YIN,Hui LIU,Shorgan BOU,Guangpeng LI

Journal Article

Effects of the

Yi FANG,Xiangwei FU,Junjie LI,Ming DU,Baoyu JIA,Jinlong ZHANG,Xiaosheng ZHANG,Shien ZHU

Journal Article

Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes

Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao

Journal Article