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Visualization of vascular ultrastructure during osteogenesis by tissue engineering technique
ZHANG Kaigang, ZENG Bingfang, ZHANG Changqing
Frontiers of Medicine 2007, Volume 1, Issue 2, Pages 181-184 doi: 10.1007/s11684-007-0034-2
Keywords: feasibility engineering osteogenesis vascularization microscopy
Xiaoyu Wang,Yuxuan Gao,Haigang Shi,Na Liu,Wei Zhang,Hongbo Li
Frontiers of Medicine 2016, Volume 10, Issue 3, Pages 286-296 doi: 10.1007/s11684-016-0456-9
Exogenic electric fields can effectively accelerate bone healing and remodeling through the enhanced migration of bone marrow mesenchymal stem cells (BMSCs) toward the injured area. This study aimed to determine the following: (1) the direction of rat BMSC (rBMSC) migration upon exposure to a direct current electric field (DCEF), (2) the optimal DCEF intensity and duration, and (3) the possible regulatory role of SDF-1/CXCR4 axis in rBMSC migration as induced by DCEF. Results showed that rBMSCs migrated to the positive electrode of the DCEF, and that the DCEF of 200 mV/mm for 4 h was found to be optimal in enhancing rBMSC migration. This DCEF strength and duration also upregulated the expression of osteoblastic genes, including ALP and OCN, and upregulated the expression of ALP and Runx2 proteins. Moreover, when CXCR4 was inhibited, rBMSC migration due to DCEF was partially blocked. These findings indicated that DCEF can effectively induce rBMSC migration. A DCEF of 200 mV/mm for 4 h was recommended because of its ability to promote rBMSC migration, proliferation, and osteogenic differentiation. The SDF-1/CXCR4 signaling pathway may play an important role in regulating the DCEF-induced migration of rBMSCs.
Keywords: DCEF migration osteogenesis differentiation rBMSCs SDF-1/CXCR-4
3D Printing of Cell-Container-Like Scaffolds for Multicell Tissue Engineering Article
Xiaoya Wang, Meng Zhang, Jingge Ma, Mengchi Xu, Jiang Chang, Michael Gelinsky, Chengtie Wu
Engineering 2020, Volume 6, Issue 11, Pages 1276-1284 doi: 10.1016/j.eng.2020.08.001
Keywords: 3D cell containers Non-contact multicellular coculture Interactions Angiogenesis Osteogenesis
Title Author Date Type Operation
Visualization of vascular ultrastructure during osteogenesis by tissue engineering technique
ZHANG Kaigang, ZENG Bingfang, ZHANG Changqing
Journal Article
Influence of the intensity and loading time of direct current electric field on the directional migration of rat bone marrow mesenchymal stem cells
Xiaoyu Wang,Yuxuan Gao,Haigang Shi,Na Liu,Wei Zhang,Hongbo Li
Journal Article