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Phylogenetic diversity of NO reductases, new tools for monitoring, and insights into NO production in natural and engineered environments

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 10, doi: 10.1007/s11783-022-1562-3

Abstract:

● 548 representative nor genes were collected to create complete phylogenetic trees.

Keywords： N2O     Greenhouse gas     NO reductase     NO dismutase     Primer     Crystal structure    

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Design and use of group-specific primers and probes for real-time quantitative PCR

Juntaek LIM, Seung Gu SHIN, Seungyong LEE, Seokhwan HWANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 28-39 doi: 10.1007/s11783-011-0302-x

Abstract: quantification, accurately quantifying the target microbial group strongly depends on the quality of the primerincreasingly routine and automated; however, one of the most important aspects, designing and selecting primerIn many cases, failed or non-specific amplification can be attributed to improperly designed primer-probeResearchers with a good command of primer and probe design can use qPCR as a valuable tool to study biodiversity

Keywords： absolute quantification     design guideline     primer     probe     real-time quantitative polymerase chain reaction    

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Redesigned Duplex RT-qPCR for the Detection of GI and GII Human Noroviruses Article

Danlei Liu, Zilei Zhang, Qingping Wu, Peng Tian, Haoran Geng, Ting Xu, Dapeng Wang

Engineering 2020, Volume 6, Issue 4,   Pages 442-448 doi: 10.1016/j.eng.2019.08.018

Abstract: The performance of ND-RT-qPCR was further evaluated by a comparison with the commonly used Kageyama primer-probe

Keywords： Human noroviruses     RT-qPCR     Redesign     Primer     Probe     Detection    

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Title Author Date Type Operation

Phylogenetic diversity of NO reductases, new tools for monitoring, and insights into NO production in natural and engineered environments

Journal Article

Design and use of group-specific primers and probes for real-time quantitative PCR

Juntaek LIM, Seung Gu SHIN, Seungyong LEE, Seokhwan HWANG

Journal Article

Redesigned Duplex RT-qPCR for the Detection of GI and GII Human Noroviruses

Danlei Liu, Zilei Zhang, Qingping Wu, Peng Tian, Haoran Geng, Ting Xu, Dapeng Wang

Journal Article