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c-Fos expression in rat brainstem following intake of sucrose or saccharin

Ke Chen, Jianqun Yan, Jinrong Li, Bo Lv, Xiaolin Zhao

Frontiers of Medicine 2011, Volume 5, Issue 3,   Pages 294-301 doi: 10.1007/s11684-011-0144-8

Abstract: To examine whether the activation of brainstem neurons during intake of a sweet tastant is due to orosensory signals or post-ingestive factors, we compared the distribution of c-Fos-like immunoreactivity (c-FLI) in the nucleus of the solitary tract (NST) and parabrachial nucleus (PBN) of brainstem following ingestion of 0.25 M sucrose or 0.005 M saccharin solutions. Immunopositive neurons were localized mainly in the middle zone of the PBN and four rostral-caudal subregions of the NST. Intake of sucrose increased the number of FLI neurons in almost every subnucleus of the PBN (F = 7.610, = 0.023), in addition to the caudal NST at the level of the area postrema (F = 10.777, = 0.003) and the NST intermediate zone (F = 7.193, = 0.014). No significant increase in the number of c-Fos positive neurons was detected in response to saccharin ingestion, although there was a trend towards a modest increase in a few select NST and PBN nuclei. These results suggest that the PBN and NST may be involved in sweet taste perception and modulation of sweet tastant intake, but the significantly enhanced intensity of Fos expression induced by sucrose indicates that PBN/NST neuronal activity is driven by the integrated effects of sweet taste sensation and post-ingestive signals.

Keywords: c-Fos     parabrachial     the nucleus of the solitary tract     sweet tastant     rat    

Proteome comparisons reveal influence of different dietary proteins on the development of rat jejunum

Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 362-372 doi: 10.15302/J-FASE-2018206

Abstract:

This study compared proteome profiles and morphological changes of rat jejunum in response to different

Keywords: Hippo signaling pathway     meat protein     PEPT1     proteome analysis     rat jejunum    

diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed of the human heavy chain and the rat

Frontiers of Medicine 2022, Volume 16, Issue 4,   Pages 584-595 doi: 10.1007/s11684-021-0844-7

Abstract: In this study, a recombinant FVIII cDNA encoding a human/rat hybrid FVIII with an enhanced procoagulantPlasmids containing human FVIII heavy chain (hHC), human light chain (hLC), and rat light chain (rLC)

Keywords: hemophilia A     adeno-associated virus (AAV)     human/rat hybrid factor VIII     gene therapy     dual chain strategy    

A modified chronic ocular hypertension rat model for retinal ganglion cell neuroprotection

Lichun Zhong

Frontiers of Medicine 2013, Volume 7, Issue 3,   Pages 367-377 doi: 10.1007/s11684-013-0266-2

Abstract:

This study aimed to modify a chronic ocular hypertension (OHT) rat model to screen for potential compoundsThe OHT rat model was created via the injection of a hypertonic saline solution into the episcleral veins

Keywords: chronic ocular hypertension     intraocular pressure     retinal ganglion cells     neuroprotection     glaucoma    

Spontaneous firing properties of rat medial vestibular nucleus neurons in brain slices by infrared visual

XIA Jiao, KONG Weijia, ZHU Yun, ZHOU Yan, ZHANG Yu, GUO Changkai

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 264-268 doi: 10.1007/s11684-008-0050-x

Abstract: paper describes the preparation procedure of brainstem slices and the spontaneous firing properties of ratBy infrared differential interference contrast technique, neurons of rat MVN were visualized directly

Keywords: resistance     infrared     infrared videomicroscopy     depressive     after-hyperpolarization potential    

Construction and identification of lentiviral RNA interference vector of rat leptin receptor gene

Zhengjuan LIU, Jie BIAN, Yuchuan WANG, Yongli ZHAO, Dong YAN, Xiaoxia WANG

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 57-60 doi: 10.1007/s11684-009-0003-z

Abstract: The aim of the present study was to construct the lentiviral RNA interference (RNAi) vector of rat OBRbreducing gene expression were further confirmed by real-time polymerase chain reaction in transfected ratspecifically reduced the expression of OBRb mRNA by approximately 80% compared with controls in transfected ratThe successful construction of rat lentivirus vectors expressing OBRb-specific shRNA may be useful for

Keywords: receptors     leptin     RNA interference     lentivirus vector    

Adenovirus-mediated antisense ERK2 gene therapy ameliorates chronic allograft nephropathy in a rat model

Zhao DING, Zhishui CHEN, Xilin CHEN, Ming CAI, Hui GUO, Nianqiao GONG

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 204-210 doi: 10.1007/s11684-009-0039-0

Abstract: To investigate the effect and underlying mechanism of adenovirus-mediated antisense ERK2 (Adanti-ERK2) gene therapy upon chronic allograft nephropathy (CAN) of rats, male Lewis (LEW, RT11) rats received male Fisher (F344, RT11v1) renal allografts. The recipients were divided into three groups: (1) empty control group; (2) vector control group; (3) gene therapy group. All recipients were sacrificed for the grafts and serum analysis at the 24th week after transplantation. Morphometric analysis was used to determine the fibrosis of grafts. Immunohistochemistry was used to detect the expression of E-Cadherin, Vimentin, TβR I and the infiltration of CD4 T lymphocyte, CD8 T lymphocyte and ED-1 monocytes. Enzyme linked immunosorbent assay (ELISA) was used to detect TGF-β1 in serum. The grafts in the control group and vector control group showed CAN. There was less E-Cadherin in renal tubular epithelial cells in the empty control group but more Vimentin and TβR I. In the gene therapy group, the fibrosis was ameliorated and fewer T lymphocytes and ED-1 monocytes infiltrated in the interstitium. There was no significant difference in the expression of E-Cadherin between the gene therapy group and normal rats. Compared with the empty control group, the expression of TGF-β1 in the gene therapy group was down-regulated. Adanti-ERK2 gene therapy protects the renal allograft and attenuates graft fibrosis, which may be correlated with a decreased renal tubular epithelial mesenchymal transition, a decreased infiltration of CD4 T lymphocyte, CD8 T lymphocytes and ED-1 monocytes in renal interstitium, and the down-regulated TGF-β1 expression.

Keywords: anti-ERK2     renal transplantation     epithelial mesenchymal transition     chronic allograft nephropathy    

Protein aggregation in association with delayed neuronal death in rat model of brain ischemia

GE Pengfei, FU Shuanglin, LI Wenchen, WANG Chonghao, ZHOU Chuibing, LUO Yinan, LUO Tianfei

Frontiers of Medicine 2008, Volume 2, Issue 1,   Pages 70-74 doi: 10.1007/s11684-008-0013-2

Abstract: To investigate the relationship between protein aggregation and delayed neuronal death, we adopted rat

ketamine with increased levels of brain-derived neurotrophic factor and tropomyosin-related kinase B in rat

Chun Yang, Xiaomin Li, Nan Wang, Shixia Xu, Jianjun Yang, Zhiqiang Zhou

Frontiers of Medicine 2012, Volume 6, Issue 4,   Pages 411-415 doi: 10.1007/s11684-012-0226-2

Abstract: ketamine-elicited antidepressant effects, which is associated with the increased levels of BDNF and TrkB in rat

Keywords: tramadol     ketamine     antidepressant     brain-derived neurotrophic factor     tropomyosin-related kinase B    

of the intensity and loading time of direct current electric field on the directional migration of rat

Xiaoyu Wang,Yuxuan Gao,Haigang Shi,Na Liu,Wei Zhang,Hongbo Li

Frontiers of Medicine 2016, Volume 10, Issue 3,   Pages 286-296 doi: 10.1007/s11684-016-0456-9

Abstract: This study aimed to determine the following: (1) the direction of rat BMSC (rBMSC) migration upon exposure

Keywords: DCEF     migration     osteogenesis differentiation     rBMSCs     SDF-1/CXCR-4    

Mechanism of hepatocellular damage in rat caused by low serum selenium

LU Yi, QU Bo, LIU Chang, YU Liang, Liu Xuemin, WANG Haohua, JIANG An, ZHANG Xiaogang

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 255-258 doi: 10.1007/s11684-008-0048-4

Abstract: The aim of this paper is to investigate the mechanism of hepatocellular damage in rats caused by low serum selenium. Thirty six rats were randomly divided into 2 groups: group A (fed with low-selenium diet from the Keshan Disease area with the content of selenium being 0.017 mg/kg); group B [fed with selenium-supplemented diet and 0.3 mg/L selenium (NaSeO) was added to the drinking water]. Both were respectively fed for 12 weeks. At the end of the 12 week, the levels of serum selenium, glutathione peroxidase (GPX) and malondialdehyde (MDA) in hepatic tissue were measured; the hepatocellular ultrastructure and apoptosis were observed as well. The levels of serum selenium and GPX in group A were markedly lower than those in group B. MDA level in group A was significantly higher than that in group B. Under the electron microscope (EM), the mitochondria were remarkably changed in group A. The rate of liver cell apoptosis appeared much higher in group A as well. It indicated that the damage caused by selenium deficiency was through the process of oxidation. Selenium deficiency led to apoptosis of hepatocytes where oxidative damage to mitochondria might be the cause.

Keywords: process     glutathione peroxidase     hepatocellular damage     group     Keshan Disease    

PET imaging on neurofunctional changes after optogenetic stimulation in a rat model of panic disorder

Xiao He, Chentao Jin, Mindi Ma, Rui Zhou, Shuang Wu, Haoying Huang, Yuting Li, Qiaozhen Chen, Mingrong Zhang, Hong Zhang, Mei Tian

Frontiers of Medicine 2019, Volume 13, Issue 5,   Pages 602-609 doi: 10.1007/s11684-019-0704-x

Abstract: Panic disorder (PD) is an acute paroxysmal anxiety disorder with poorly understood pathophysiology. The dorsal periaqueductal gray (dPAG) is involved in the genesis of PD. However, the downstream neurofunctional changes of the dPAG during panic attacks have yet to be evaluated . In this study, optogenetic stimulation to the dPAG was performed to induce panic-like behaviors, and positron emission tomography (PET) imaging with F-flurodeoxyglucose ( F-FDG) was conducted to evaluate neurofunctional changes before and after the optogenetic stimulation. Compared with the baseline, post-optogenetic stimulation PET imaging demonstrated that the glucose metabolism significantly increased ( <0.001) in dPAG, the cuneiform nucleus, the cerebellar lobule, the cingulate cortex, the alveus of the hippocampus, the primary visual cortex, the septohypothalamic nucleus, and the retrosplenial granular cortex but significantly decreased ( <0.001) in the basal ganglia, the frontal cortex, the forceps minor corpus callosum, the primary somatosensory cortex, the primary motor cortex, the secondary visual cortex, and the dorsal lateral geniculate nucleus. Taken together, these data indicated that PET imaging can successfully detect downstream neurofunctional changes involved in the panic attacks after optogenetic stimulation to the dPAG.

Keywords: panic disorder (PD)     positron emission tomography (PET)     optogenetics     dorsal periaqueductal gray (dPAG)    

Effects of hypoxia on mRNA expression of housekeeping genes in rat brain tissue and primary cultured

YANG Yingzhong, MA Lan, GE Rili, FAN Wenhong, ZHU Lingling, ZHAO Tong, WU Yan, FAN Ming

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 239-243 doi: 10.1007/s11684-008-0045-7

Abstract: Internal standards are critical for quantitative RNA analyses. Housekeeping genes are often used as internal standards with the assumption that their expression levels remain relatively constant in different experimental conditions. In this study, four commonly used housekeeping genes, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ?-actin, 28S rRNA and 18S rRNA were selected to test whether this assumption is tenable under hypoxic conditions. We tested the RNA expression level of these four genes in different hypoxic conditions. Rats subjected to acute hypoxia for 2 hours were used for tissue detection. Primary cultured neural stem cells from E13 fetal rats were treated with 3% O or 10% O for 24 hours for experiments. In both experiments, expression levels of 28S rRNA and 18S rRNA were constant, independent of hypoxia types. However, expression levels of GAPDH and ?-actin were all changed in all kinds of hypoxic conditions. In particular, the mRNA expression level of GAPDH was increased by 43.4% under 3% O hypoxic conditions. These results suggest that 18S rRNA and 28S rRNA are reliable internal controls for comparative analyses of transcription under hypoxia. GAPDH appears particularly unfavorable for this purpose in hypoxic conditions.

Keywords: Glyceraldehyde-3-phosphate dehydrogenase     comparative     constant     unfavorable     independent    

iTRAQ-based quantitative proteomic analysis on differentially expressed proteins of rat mandibular condylar

Liting Jiang,Yinyin Xie,Li Wei,Qi Zhou,Ning Li,Xinquan Jiang,Yiming Gao

Frontiers of Medicine 2017, Volume 11, Issue 1,   Pages 97-109 doi: 10.1007/s11684-016-0496-1

Abstract: structural changes of MCC and differentially expressed proteins induced by reducing functional loading in rat

Keywords: condylar cartilage     mechanical loading     proteomic analysis     iTRAQ     bioinformatics analysis    

Separation,Purification and Identification of Rat Bone Marrow Mesenchymal Stem Cells

Zhang Rongli,Feng Xue,Zhang Huiliang,Luo Guoan,Li Lianda ,Wang Yiming

Strategic Study of CAE 2007, Volume 9, Issue 7,   Pages 89-94

Abstract:

Bone marrow mesenchymal stem cells (BMMSCs) were separated by density gradient centrifugation and purified by different adherence speed. BMMSC was identified by flow cytometry analysis and laser confocal microscopy. Cell surface antigen (CSA) CD29, CD44, CD105, CD166 were detected positive expression and CD14, CD34, CD45 were not positive. The positive expression of the nestin, NST, and weakly positive expression of Oct-4 were confirmed by RT-PCR. The cells were verified as the BMMSC by the expression of the CSA and the genes. Using density gradient separation and different adherence speed could harvest high quality BMMSC. This is a simple and reliable method to obtain BMMSC as stem cell used in cell and tissue engineering research.

Keywords: rat     bone marrow mesenchymal stem cells(BMMSCs)     flow cytometry    

Title Author Date Type Operation

c-Fos expression in rat brainstem following intake of sucrose or saccharin

Ke Chen, Jianqun Yan, Jinrong Li, Bo Lv, Xiaolin Zhao

Journal Article

Proteome comparisons reveal influence of different dietary proteins on the development of rat jejunum

Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU

Journal Article

diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed of the human heavy chain and the rat

Journal Article

A modified chronic ocular hypertension rat model for retinal ganglion cell neuroprotection

Lichun Zhong

Journal Article

Spontaneous firing properties of rat medial vestibular nucleus neurons in brain slices by infrared visual

XIA Jiao, KONG Weijia, ZHU Yun, ZHOU Yan, ZHANG Yu, GUO Changkai

Journal Article

Construction and identification of lentiviral RNA interference vector of rat leptin receptor gene

Zhengjuan LIU, Jie BIAN, Yuchuan WANG, Yongli ZHAO, Dong YAN, Xiaoxia WANG

Journal Article

Adenovirus-mediated antisense ERK2 gene therapy ameliorates chronic allograft nephropathy in a rat model

Zhao DING, Zhishui CHEN, Xilin CHEN, Ming CAI, Hui GUO, Nianqiao GONG

Journal Article

Protein aggregation in association with delayed neuronal death in rat model of brain ischemia

GE Pengfei, FU Shuanglin, LI Wenchen, WANG Chonghao, ZHOU Chuibing, LUO Yinan, LUO Tianfei

Journal Article

ketamine with increased levels of brain-derived neurotrophic factor and tropomyosin-related kinase B in rat

Chun Yang, Xiaomin Li, Nan Wang, Shixia Xu, Jianjun Yang, Zhiqiang Zhou

Journal Article

of the intensity and loading time of direct current electric field on the directional migration of rat

Xiaoyu Wang,Yuxuan Gao,Haigang Shi,Na Liu,Wei Zhang,Hongbo Li

Journal Article

Mechanism of hepatocellular damage in rat caused by low serum selenium

LU Yi, QU Bo, LIU Chang, YU Liang, Liu Xuemin, WANG Haohua, JIANG An, ZHANG Xiaogang

Journal Article

PET imaging on neurofunctional changes after optogenetic stimulation in a rat model of panic disorder

Xiao He, Chentao Jin, Mindi Ma, Rui Zhou, Shuang Wu, Haoying Huang, Yuting Li, Qiaozhen Chen, Mingrong Zhang, Hong Zhang, Mei Tian

Journal Article

Effects of hypoxia on mRNA expression of housekeeping genes in rat brain tissue and primary cultured

YANG Yingzhong, MA Lan, GE Rili, FAN Wenhong, ZHU Lingling, ZHAO Tong, WU Yan, FAN Ming

Journal Article

iTRAQ-based quantitative proteomic analysis on differentially expressed proteins of rat mandibular condylar

Liting Jiang,Yinyin Xie,Li Wei,Qi Zhou,Ning Li,Xinquan Jiang,Yiming Gao

Journal Article

Separation,Purification and Identification of Rat Bone Marrow Mesenchymal Stem Cells

Zhang Rongli,Feng Xue,Zhang Huiliang,Luo Guoan,Li Lianda ,Wang Yiming

Journal Article