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Exome sequencing greatly expedites the progressive research of Mendelian diseases

Xuejun Zhang

Frontiers of Medicine 2014, Volume 8, Issue 1,   Pages 42-57 doi: 10.1007/s11684-014-0303-9

Abstract:

The advent of whole-exome sequencing (WES) has facilitated the discovery of rare structure and functionalCombining exome sequencing with linkage studies is one of the most efficient strategies in searchingWe illustrate the workflow of exome capture and sequencing to highlight the advantages of WES.

Keywords： genetics     whole-exome sequencing     Mendelian disease     disease gene    

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TCGA whole-transcriptome sequencing data reveals significantly dysregulated genes and signaling pathways

Daniel Wai-Hung Ho,Alan Ka-Lun Kai,Irene Oi-Lin Ng

Frontiers of Medicine 2015, Volume 9, Issue 3,   Pages 322-330 doi: 10.1007/s11684-015-0408-9

Abstract:

This study systematically evaluates the TCGA whole-transcriptome sequencing data of hepatocellular

Keywords： TCGA     whole-transcriptome sequencing     HCC     liver cancer    

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Accurate quantification of 3′-terminal 2′-O-methylated small RNAs by utilizing oxidative deep sequencing

Frontiers of Medicine 2022, Volume 16, Issue 2,   Pages 240-250 doi: 10.1007/s11684-021-0909-7

Abstract: , we profiled 3′-terminal 2′-O-methyl plant miRNAs in the livers of rice-fed mice by oxidative deep sequencing

Keywords： small RNAs     2′-O-methylation     sequencing     RT-qPCR    

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Exploring the cancer genome in the era of next-generation sequencing

Hui Dong, Shengyue Wang

Frontiers of Medicine 2012, Volume 6, Issue 1,   Pages 48-55 doi: 10.1007/s11684-012-0182-x

Abstract:

The emergence of next-generation sequencing technologies has led to dramatic advances in cancer genomeThe increased efficiency and resolution of next-generation sequencing greatly facilitate the detectionComprehensive analysis of cancer genomes through approaches of whole genome, exome, and transcriptome sequencingThe present study briefly reviews the recent pioneering studies on cancer genome sequencing and provides

Keywords： next-generation sequencing     cancer genome     whole genome sequencing     exome     transcriptome    

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Comparative study on microbial community in intermittently aerated sequencing batch reactors (SBR) and

Xiaolin Sheng, Rui Liu, Xiaoyan Song, Lujun Chen, Kawagishi Tomoki

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 3, doi: 10.1007/s11783-017-0929-3

Abstract: A traditional sequencing batch reactor (SBR) and two intermittently aerated sequencing batch reactors

Keywords： wastewater     Intermittent aeration     Microbial community     Partial nitrification–denitrification process     Sequencing    

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METAGENOMICS COMBINED WITH HIGH-THROUGHPUT SEQUENCING REVEALS THE METHANOGENIC POTENTIAL OF FRESH CORN

Frontiers of Agricultural Science and Engineering doi: 10.15302/J-FASE-2022471

Abstract:

● Methane production from fresh straw was 7.50% higher than dry straw.

Keywords： fresh corn straw     high solid anaerobic digestion     metagenomics     microbial communities     thermophilic    

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Improvement of nitrification efficiency by bioaugmentation in sequencing batch reactors at low temperature

Di CUI,Ang LI,Tian QIU,Rui CAI,Changlong PANG,Jihua WANG,Jixian YANG,Fang MA,Nanqi REN

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 6,   Pages 937-944 doi: 10.1007/s11783-014-0668-7

Abstract: Bioaugmentation is an effective method of treating municipal wastewater with high ammonia concentration in sequencing

Keywords： nitrification     sequencing batch reactors (SBRs)     bioaugmentation     low temperature    

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Simultaneous denitrifying phosphorus accumulation in a sequencing batch reactor

YUAN Linjiang, HAN Wei, WANG Lei, YANG Yongzhe, WANG Zhiying

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 1,   Pages 23-27 doi: 10.1007/s11783-007-0004-6

Abstract: treatment process, denitrifying phosphorus accumulation (DNPA) and its affecting factors were studied in a sequencing

Keywords： SBR     synthetic wastewater     Induced DNPA     resource     removal ability    

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Effects of La, Ce on nitrogen removal in sequencing batch reactor

Qing XIA , Rui LIANG , Yuning HONG , Lili DING , Hongqiang REN , Yuxiang MAO , Mingyu ZHAO ,

Frontiers of Environmental Science & Engineering 2009, Volume 3, Issue 3,   Pages 369-374 doi: 10.1007/s11783-009-0036-1

Abstract: short-term biological effects of rare earth ions (La, Ce) and their mixture on the nitrogen removal in a sequencing

Keywords： rare earth     La3+     Ce3+     inorganic nitrogen     nitrogen removal     sequencing batch reactor    

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Nitrifying and denitrifying bacteria in aerobic granules formed in sequencing batch airlift reactors

WANG Fang, YANG Fenglin, QI Aijiu

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 2,   Pages 184-189 doi: 10.1007/s11783-007-0032-2

Abstract: Aerobic granules were formed in an internal-circulate sequencing batch airlift reactor (SBAR) and biodegradation
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microbial composition during wastewater reclamation and distribution systems revealed by high-throughput sequencing

Yiwen LIN,Dan LI,Siyu ZENG,Miao HE

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 3,   Pages 539-547 doi: 10.1007/s11783-016-0830-5

Abstract: This study employed 454-pyrosequencing to investigate microbial and pathogenic communities in two wastewater reclamation and distribution systems. A total of 11972 effective 16S rRNA sequences were acquired from these two reclamation systems, and then designated to relevant taxonomic ranks by using RDP classifier. The Chao index and Shannon diversity index showed that the diversities of microbial communities decreased along wastewater reclamation processes. was the most dominant phylum in reclaimed water after disinfection, which accounted for 83% and 88% in two systems, respectively. Human opportunistic pathogens, including , , and , were selected and enriched by disinfection processes. The total chlorine and nutrients (TOC, NH -N and NO -N) significantly affected the microbial and pathogenic communities during reclaimed water storage and distribution processes. Our results indicated that the disinfectant-resistant pathogens should be controlled in reclaimed water, since the increases in relative abundances of pathogenic bacteria after disinfection implicate the potential public health associated with reclaimed water.

Keywords： wastewater reclamation systems     microbial community     pathogenic community     454-pyrosequencing    

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N2O emission from a sequencing batch reactor for biological N and P removal from wastewater

Lei SHEN,Yuntao GUAN,Guangxue WU,Xinmin ZHAN

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 5,   Pages 776-783 doi: 10.1007/s11783-013-0586-0

Abstract: N O emission from a sequencing batch reactor (SBR) for biological nitrogen and phosphorus removal from

Keywords： biological nutrient removal     denitrification     greenhouse gas     nitrification     nitrous oxide    

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Advanced nitrogen removal by pulsed sequencing batch reactors (SBR) with real-time control

YANG Qing, PENG Yongzhen, YANG Anming, LI Jianfeng, GUO Jianhua

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 4,   Pages 488-492 doi: 10.1007/s11783-007-0078-1

Abstract: oxidation reduction potential (ORP) as on-line control parameters to advance nitrogen removal in pulsed sequencing

Keywords： reduction potential     oxidation reduction     operational     feasibility     nitrogen    

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PathogenTrack and Yeskit: tools for identifying intracellular pathogens from single-cell RNA-sequencing

Frontiers of Medicine 2022, Volume 16, Issue 2,   Pages 251-262 doi: 10.1007/s11684-021-0915-9

Abstract: Taking advantage of single-cell RNA-sequencing (scRNA-seq) analysis, we can assess the transcriptomic

Keywords： scRNA-seq     intracellular pathogen     microbe     COVID-19     SARS-CoV-2    

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Mechanism studies on nitrogen removal when treating ammonium-rich leachate by sequencing batch biofilm

XU Zhengyong, YANG Zhaohui, ZENG Guangming, XIAO Yong, DENG Jiuhua

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 1,   Pages 43-48 doi: 10.1007/s11783-007-0008-2

Abstract: removal mechanism was studied and analyzed when treating the ammonium-rich landfill leachate by a set of sequencing

Keywords： undulation     33-days stabilization     concentration debasement     temperature     accumulation    

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Title Author Date Type Operation

Exome sequencing greatly expedites the progressive research of Mendelian diseases

Xuejun Zhang

Journal Article

TCGA whole-transcriptome sequencing data reveals significantly dysregulated genes and signaling pathways

Daniel Wai-Hung Ho,Alan Ka-Lun Kai,Irene Oi-Lin Ng

Journal Article

Accurate quantification of 3′-terminal 2′-O-methylated small RNAs by utilizing oxidative deep sequencing

Journal Article

Exploring the cancer genome in the era of next-generation sequencing

Hui Dong, Shengyue Wang

Journal Article

Comparative study on microbial community in intermittently aerated sequencing batch reactors (SBR) and

Xiaolin Sheng, Rui Liu, Xiaoyan Song, Lujun Chen, Kawagishi Tomoki

Journal Article

METAGENOMICS COMBINED WITH HIGH-THROUGHPUT SEQUENCING REVEALS THE METHANOGENIC POTENTIAL OF FRESH CORN

Journal Article

Improvement of nitrification efficiency by bioaugmentation in sequencing batch reactors at low temperature

Di CUI,Ang LI,Tian QIU,Rui CAI,Changlong PANG,Jihua WANG,Jixian YANG,Fang MA,Nanqi REN

Journal Article

Simultaneous denitrifying phosphorus accumulation in a sequencing batch reactor

YUAN Linjiang, HAN Wei, WANG Lei, YANG Yongzhe, WANG Zhiying

Journal Article

Effects of La, Ce on nitrogen removal in sequencing batch reactor

Qing XIA , Rui LIANG , Yuning HONG , Lili DING , Hongqiang REN , Yuxiang MAO , Mingyu ZHAO ,

Journal Article

Nitrifying and denitrifying bacteria in aerobic granules formed in sequencing batch airlift reactors

WANG Fang, YANG Fenglin, QI Aijiu

Journal Article

microbial composition during wastewater reclamation and distribution systems revealed by high-throughput sequencing

Yiwen LIN,Dan LI,Siyu ZENG,Miao HE

Journal Article

N2O emission from a sequencing batch reactor for biological N and P removal from wastewater

Lei SHEN,Yuntao GUAN,Guangxue WU,Xinmin ZHAN

Journal Article

Advanced nitrogen removal by pulsed sequencing batch reactors (SBR) with real-time control

YANG Qing, PENG Yongzhen, YANG Anming, LI Jianfeng, GUO Jianhua

Journal Article

PathogenTrack and Yeskit: tools for identifying intracellular pathogens from single-cell RNA-sequencing

Journal Article

Mechanism studies on nitrogen removal when treating ammonium-rich leachate by sequencing batch biofilm

XU Zhengyong, YANG Zhaohui, ZENG Guangming, XIAO Yong, DENG Jiuhua

Journal Article