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Journal Article 4

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2023 2

2011 1

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Electrochemical corrosion 1

Electrolytes 1

Inflammation 1

Ionic conductors 1

Joule heating 1

Macrophage activation 1

Macrophage migration 1

Multimodal identification 1

SPRED1 1

TB 1

Xuanfeibaidu Formula 1

Zero-phase frequency 1

decoloration 1

diverse ions 1

lncRNA LOC646029 1

metastasis 1

microRNA 627-3p 1

ovarian cancer 1

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Long noncoding RNA LOC646029 functions as a ceRNA to suppress ovarian cancer progression through the

Frontiers of Medicine   Pages 924-938 doi: 10.1007/s11684-023-1004-z

Abstract: Long noncoding RNAs (lncRNAs) play a crucial regulatory role in the development and progression of multiple cancers. However, the potential mechanism by which lncRNAs affect the recurrence and metastasis of ovarian cancer remains unclear. In the current study, the lncRNA LOC646029 was markedly downregulated in metastatic ovarian tumors compared with primary tumors. Gain- and loss-of-function assays demonstrated that LOC646029 inhibits the proliferation, invasiveness, and metastasis of ovarian cancer cells in vivo and in vitro. Moreover, the downregulation of LOC646029 in metastatic ovarian tumors was strongly correlated with poor prognosis. Mechanistically, LOC646029 served as a miR-627-3p sponge to promote the expression of Sprouty-related EVH1 domain-containing protein 1, which is necessary for suppressing tumor metastasis and inhibiting KRAS signaling. Collectively, our results demonstrated that LOC646029 is involved in the progression and metastasis of ovarian cancer, which may be a potential prognostic biomarker.

Keywords: ovarian cancer     lncRNA LOC646029     metastasis     microRNA 627-3p     SPRED1    

Spectrokinetics study of probable effects of diverse inorganic ions on bleaching of dye

Rafia AZMAT, Masooda QADRI, Fahim UDDIN

Frontiers of Chemical Science and Engineering 2011, Volume 5, Issue 1,   Pages 131-138 doi: 10.1007/s11705-010-0556-z

Abstract: Toluidine blue (TB) is an important anticoagulant metachromasia molecule showing a pronounced variation in the visible spectrum due to the aggregation phenomenon and electrostatic interaction with the charged synthetic and biologic polymers. The current study describes the interactive role of diverse inorganic material ions on the bleaching of toluidine blue (tolonium chloride) (TB ) with urea in acidic and basic media using the spectrophotometric technique. The spectra of TB and urea with different cations and anions were monitored and their characteristic features are presented here. The negative effect of added cations on reduction may be the result of altered electron pathways which led to suppression of the reduction/bleaching of TB, while a slight decrease in dye reduction by added anions may be due to the scavenging of the OH* radical. It has been observed in the case of Co that in addition to the electron-transfer reaction, other processes like layer and precipitate formation also appear to be taking place. The dye bleaching process followed pseudo first order kinetics with respect to TB, urea, and H ion, whereas significant decoloration in the presence of urea proved that reductants control the redox reaction. No decoloration in acidic medium with diverse ions was seen compared to alkaline media, showing that water pH played an important role in the bleaching of dye. The reduction/bleaching of dye was investigated at different temperatures, and energy parameters were evaluated for a TB -Urea reaction, including the energy of activation ( = 39.60 kJ·mol ), enthalpy of activation (? = 34?kJ·mol ), entropy of activation (? = 146.5 kJ mol ·K ), and free energy of activation (ΔG* = -52.35 kJ·mol ). A mechanism of interaction of diverse ions in dye bleaching and a mechanism of reduction based on the above findings is proposed.

Keywords: TB     diverse ions     suppress     decoloration    

Joule Heating of Ionic Conductors Using Zero-Phase Frequency Alternating Current to Suppress Electrochemical Letter

Lei Shi, Zongyi Han, Yixuan Feng, Changgeng Zhang, Qi Zhang, He Zhu, Shiping Zhu

Engineering 2023, Volume 25, Issue 6,   Pages 138-143 doi: 10.1016/j.eng.2022.03.004

Abstract: Electricity with a zero-phase frequency is employed to suppress the electrolysis of ionic conductors

Keywords: Joule heating     Ionic conductors     Electrolytes     Zero-phase frequency     Electrochemical corrosion    

Identification by Transcriptomics and Multiscale Bioassays of Active Components in Xuanfeibaidu Formula to Suppress Article

Lu Zhao, Hao Liu, Yingchao Wang, Shufang Wang, Dejin Xun, Yi Wang, Yiyu Cheng, Boli Zhang

Engineering 2023, Volume 20, Issue 1,   Pages 63-76 doi: 10.1016/j.eng.2021.09.007

Abstract:

Xuanfeibaidu Formula (XFBD) is a Chinese medicine used in the clinical treatment of coronavirus disease 2019 (COVID-19) patients. Although XFBD has exhibited significant therapeutic efficacy in clinical practice, its underlying pharmacological mechanism remains unclear. Here, we combine a comprehensive research approach that includes network pharmacology, transcriptomics, and bioassays in multiple model systems to investigate the pharmacological mechanism of XFBD and its bioactive substances. High-resolution mass spectrometry was combined with molecular networking to profile the major active substances in XFBD. A total of 154 compounds were identified or tentatively characterized, including flavonoids, terpenes, carboxylic acids, and other types of constituents. Based on the chemical composition of XFBD, a network pharmacology-based analysis identified inflammation-related pathways as primary targets. Thus, we examined the anti-inflammation activity of XFBD in a lipopolysaccharide-induced acute inflammation mice model. XFBD significantly alleviated pulmonary inflammation and decreased the level of serum proinflammatory cytokines. Transcriptomic profiling suggested that genes related to macrophage function were differently expressed after XFBD treatment. Consequently, the effects of XFBD on macrophage activation and mobilization were investigated in a macrophage cell line and a zebrafish wounding model. XFBD exerts strong inhibitory effects on both macrophage activation and migration. Moreover, through multimodal screening, we further identified the major components and compounds from the different herbs of XFBD that mediate its anti-inflammation function. Active components from XFBD, including Polygoni cuspidati Rhizoma, Phragmitis Rhizoma, and Citri grandis Exocarpium rubrum, were then found to strongly downregulate macrophage activation, and polydatin, isoliquiritin, and acteoside were identified as active compounds. Components of Artemisiae annuae Herba and Ephedrae Herba were found to substantially inhibit endogenous macrophage migration, while the presence of ephedrine, atractylenolide, and kaempferol was attributed to these effects. In summary, our study explores the pharmacological mechanism and effective components of XFBD in inflammation regulation via multimodal approaches, and thereby provides a biological illustration of the clinical efficacy of XFBD.

Keywords: Xuanfeibaidu Formula     Multimodal identification     Inflammation     Macrophage activation     Macrophage migration    

Title Author Date Type Operation

Long noncoding RNA LOC646029 functions as a ceRNA to suppress ovarian cancer progression through the

Journal Article

Spectrokinetics study of probable effects of diverse inorganic ions on bleaching of dye

Rafia AZMAT, Masooda QADRI, Fahim UDDIN

Journal Article

Joule Heating of Ionic Conductors Using Zero-Phase Frequency Alternating Current to Suppress Electrochemical

Lei Shi, Zongyi Han, Yixuan Feng, Changgeng Zhang, Qi Zhang, He Zhu, Shiping Zhu

Journal Article

Identification by Transcriptomics and Multiscale Bioassays of Active Components in Xuanfeibaidu Formula to Suppress

Lu Zhao, Hao Liu, Yingchao Wang, Shufang Wang, Dejin Xun, Yi Wang, Yiyu Cheng, Boli Zhang

Journal Article