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2013年 第7卷 第4期

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《化学科学与工程前沿（英文）》 >> 2013年 第7卷 第4期 doi: 10.1007/s11705-013-1342-5

Production of pectic extracts from sugar beet pulp with antiproliferative activity on a breast cancer cell line

1. Department of Biochemistry, Pharmacy School, Universidad de Valparaíso, Gran Breta?a 1093, Playa Ancha, Valparaíso, Chile; 2. School of Biochemistry Engineering, Pontificia Universidad Católica de Valparaíso, General Cruz 34, Valparaíso, Chile; 3. Creas Conicyt-Regional Gore Región de Valparaíso R12C1001, Blanco 1623, of 1402, Valparaíso, Chile

发布日期： 2013-12-05
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In the last years, sugar beet pectins have been the subject of several investigations involving extraction methodologies, chemical composition and functional properties. The structure of pectins, which depends on the extraction method, is decisive in their capacity to induce apoptosis on several cancer cell lines like colon, prostate and breast. In this work, sugar beet pectin extraction was performed in the following steps: lipid extraction with hexane, removal of soluble complex carbohydrates and proteins, and enzymatic treatment with amyloglucosidase, protease, and pectinase. The enzymatic treatment was carried out with Rohapect DA6L under the following conditions: 50°C, pH 4.0, 2% enzyme/substrate (E/S) ratio, 15 h, and a solid to liquid ratio of 1 ∶ 10. The pectic extract showed a degree of polymerization (DP) profile of 55.8% with DP≥7; 4.9% with DP6; 5.8% between DP2 and DP6 ; 4.7% with DP2; and 28.8% with DP1. The pectic extract was examined for its antiproliferative activity on the MCF-7 breast cancer cell line. At a concentration range of 12.5–25 mg/mL the pectic extract killed 80.6% of the cells, exhibiting a higher antiproliferative activity than 4-hydroxytamoxifen (4-OHT), a classical anticancer drug, which killed 56.5% of the cells.

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