期刊首页 优先出版 当期阅读 过刊浏览 作者中心 关于期刊 English

2014年 第8卷 第3期

摘要

关键词

相关研究

回顶部

《化学科学与工程前沿（英文）》 >> 2014年 第8卷 第3期 doi: 10.1007/s11705-014-1421-2

Immobilization of

1. Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.2. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China

发布日期： 2014-10-11
HTML23 PDF 1 收藏 0

下一篇 上一篇

摘要

Mesoporous silica particles were prepared for efficient immobilization of the -glucuronidase (GUS) through a biomimetic mineralization process, in which the solution containing lysozyme and GUS were added into the prehydrolyzed tetraethoxysilane (TEOS) solution. The silica particles were formed in a way of biomineralization under the catalysis of lysozyme and GUS was immobilized into the silica particles simultaneously during the precipitation process. The average diameter of the silica particles is about 200 nm with a pore size of about 4 nm. All the enzyme molecules are tightly entrapped inside the biosilica nanoparticles without any leaching even under a high ionic strength condition. The immobilized GUS exhibits significantly higher thermal and pH stability as well as the storage and recycling stability compared with GUS in free form. No loss in the enzyme activity of the immobilized GUS was found after 30-day’s storage, and the initial activity could be well retained after 12 repeated cycles.

相关研究