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《工程(英文)》 >> 2021年 第7卷 第2期 doi: 10.1016/j.eng.2020.10.010

解淀粉芽孢杆菌发酵红小豆的理化特性和生物活性

a Key Laboratory of Food Bioengineering (China National Light Industry), College of Engineering, China Agricultural University, Beijing 100083, China
b Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

收稿日期: 2018-11-02 修回日期: 2018-12-12 录用日期: 2018-12-18 发布日期: 2020-10-29

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摘要

本研究采用解淀粉芽孢杆菌发酵红小豆(Vigna umbellata),并评价了发酵红小豆的理化特性和生物活性。以纤溶酶活性为指标优化了发酵条件,在最适发酵条件下,纤溶酶活性最高达78.0 FU∙g-1(4890 IU∙g-1,纤维蛋白平板法)。发酵红小豆中多肽含量(2.1~10.9 g∙100 g-1)、总酚含量(116.7~388.5 mg没食子酸∙100 g-1)、总黄酮含量(235.5~354.3 mg芦丁∙100 g-1)、花青素含量(20.1~47.1 mg∙100 g-1)和超氧化物歧化酶活性(55.3~263.6 U∙g-1)显著增加。红小豆经发酵后的2,2-二苯基-1-苦肼基(DPPH)和2,2-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基清除活性和铁离子还原抗氧化能力(FRAP)较未发酵的红小豆提高1.9~4.8倍。此外,发酵红小豆的二肽基肽酶Ⅳ(DPP-Ⅳ)抑制活性、α-葡萄糖苷酶抑制活性和抗凝血活性显著提高。解淀粉芽孢杆菌发酵红小豆具有多种功能活性,对开发预防血栓性疾病的功能食品具有重要意义。

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