2022年 第10卷 第3期
《工程（英文）》 >> 2022年 第10卷 第3期 doi: 10.1016/j.eng.2022.02.001
在临床批准的免疫抑制剂药物作用下胎猪肾脏在成熟猴体内的发育情况
a Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan
b Department of Urology, The Jikei University School of Medicine, Tokyo 105-8461, Japan
c Department of Plastic and Reconstructive Surgery, The Jikei University School of Medicine, Tokyo 105-8461, Japan
d Meiji University International Institute for Bio-Resource Research, Kanagawa 214-8571, Japan
e Sumitomo Dainippon Pharma Co., Ltd., Osaka-fu 564-0053, Japan
f Department of Kidney Regenerative Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan
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摘要
在从猪到非人灵长类动物的肾脏异种移植中，仅使用临床批准的免疫抑制药物来控制免疫反应是困难的。此外，据我们所知，目前还没有关于使用胎猪作为肾脏供体的报道。本研究旨在比较新生猪和胎猪肾脏之间的移植排斥程度，以遗传未修饰的猪作为供体，将食蟹猴作为受体。切除受体食蟹猴的左肾，然后将在同一部位进行了血管吻合的新生猪和胎猪的肾脏移植到食蟹猴左肾腹膜。仅使用美国食品药品监督管理局批准的药物进行免疫抑制。将胎猪肾脏移植到食蟹猴的网膜和腹主动脉旁区域。随时间取样（每个实验取样两次），对移植组织的移植成活率和发育进行病理学检查。几周后，观察到血管吻合的新生猪肾脏在移植后出现急性排斥反应。尽管对食蟹猴和流入胎猪肾脏的受体血管给予相同的免疫抑制方案，但胎猪的肾脏仍免于排斥。猪-猴肾脏异种移植中胎猪肾脏的免疫原性低于新生猪肾脏。
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