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《工程(英文)》 >> 2023年 第26卷 第7期 doi: 10.1016/j.eng.2022.09.012

基于正交质谱的N-糖组谱揭示哈夫病潜在病原学

a The Key Laboratory for Biomedical Photonics of the MOE at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics and Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
b Wuhan Center for Disease Control & Prevention, Wuhan 430015, China

收稿日期: 2022-05-15 修回日期: 2022-09-12 录用日期: 2022-09-29 发布日期: 2022-11-15

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摘要

食用煮熟的小龙虾可能导致哈夫病(HD),该病被认为是由不明毒素引起的,而病因尚不清楚。N-糖组的剖析将有助于破译疾病的分子机制,而HD相关的糖基化从未被探索过。2019—2020 年,本研究团队招募了来自武汉市疾病预防控制中心的90 份HD患者和对照组血清样本。本文中,采用基于高通量的正交质谱对HD中血清和血清衍生的免疫球蛋白G(IgG) N-糖组谱进行了表征。数据显示,HD与总血清糖蛋白的核心岩藻糖基化和单半乳糖基化升高有关。血清IgG 水平是诊断HD患者的良好指标。此外,IgG的差异半乳糖基化和唾液酸化与HD密切相关。值得注意的是,IgG1 和IgG2 的半乳糖基化和唾液酸化的变化具有亚类特异性。有意义的是,IgG2 或IgG3/4 的唾液酸化和半乳糖基化改变与HD的临床标志物密切相关。本研究揭示了差异化IgG N-糖基化与HD的关联,为这种罕见疾病的病因提供了新的见解。

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