表面张力辅助去核法对小鼠卵母细胞的去核研究
中国农业大学动物科技学院胚胎生物技术实验室,北京 100094
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摘要
论述了一种新的小鼠卵母细胞去核方法一一表面张力辅助去核法(STA),并同时与其他3种去核方 法(简称A法、B法、C法)进行比较。STA法是将卵母细胞核在固定管的辅助下,借助液体表面张力通过透 明带上的破口被挤出。经STA法去核的卵母细胞质体用于电融合介导的小鼠耳成纤维细胞核移植实验,结果有 76.1 %细胞与细胞质体融合形成重组胚,85.4 %的重组胚形成原核,49.4%卵裂形成2-细胞。
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