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《农业科学与工程前沿(英文)》 2022年 第9卷 第1期 doi: 10.15302/J-FASE- 2021390
It has previously been shown that tryptophan, the biosynthesis precursor of Peganum harmala alkaloids, and its derivatives have anti-TMV activity both in vitro and in vivo. Further exploration of this led to the identification of NK0238 as a highly effective agent for the prevention and control of diseases caused by plant viruses, but the existing routes are unsuitable for its large-scale synthesis. This study optimized a route for two-step synthesis of this virucide candidate via reaction of l-tryptophan with triphosgene to produce l-tryptophan-N-carboxylic anhydride, which then reacts with n-octylamine to give NK0238 at up to 94% yield and nearly 97% HPLC purity. In addition, the route was used for the preparation of NK0238 on a>40 g scale permitting further assessment of its antivirus activity in the greenhouse and field experiments, and toxicity tests. NK0238 exhibited useful antiviral activities against a variety of viruses both in greenhouse and field experiments. The toxicity tests showed that NK0238 was not acutely toxic to birds, fish, honey bees and silkworms. The optimized route provides a solid foundation for its large-scale synthesis and subsequent efficacy and toxicity studies, its excellent activity and safety make NK0238 a promising drug candidate for further development.
Wentao XU, Hao TIAN, Hongjian SONG, Yuxiu LIU, Yongqiang LI, Qingmin WANG
《农业科学与工程前沿(英文)》 页码 110-119 doi: 10.15302/J-FASE-2021390
关键词: antiviral plant protectant antiviral in the greenhouse field evaluation L-trp-NCA synthesis optimization toxicity tests
Comments on the special issue on forestry of
John L. INNES
《农业科学与工程前沿(英文)》 2017年 第4卷 第4期 页码 502-503 doi: 10.15302/J-FASE-2017189
PD-1/PD-L1 blockade in cervical cancer: current studies and perspectives
Yumeng Wang, Guiling Li
《医学前沿(英文)》 2019年 第13卷 第4期 页码 438-450 doi: 10.1007/s11684-018-0674-4
关键词: PD-1 PD-L1 immune checkpoint blockade antibody immunotherapy cervical cancer
Gridded usage inventories of chlordane in China
Qiang WANG, Lijuan ZHAO, Xuekun FANG, Jianhua XU, Yifan LI, Yehong SHI, Jianxin HU
《环境科学与工程前沿(英文)》 2013年 第7卷 第1期 页码 10-18 doi: 10.1007/s11783-012-0458-z
关键词: chlordane inventories termites China new construction area (NCA)
In memory of Perry L. McCarty, seminal founder of Environmental Biotechnology
《环境科学与工程前沿(英文)》 2023年 第17卷 第10期 doi: 10.1007/s11783-023-1730-0
Shear design of high strength concrete prestressed girders
Emad L. LABIB,Hemant B. DHONDE,Thomas T. C. HSU,Y. L. MO
《结构与土木工程前沿(英文)》 2014年 第8卷 第4期 页码 373-387 doi: 10.1007/s11709-014-0087-7
关键词: shear design high strength concrete prestressed girders full-scale tests
Deeply missing my career idol and role model ― Professor Perry L. McCarty
《环境科学与工程前沿(英文)》 2023年 第17卷 第9期 doi: 10.1007/s11783-023-1717-x
Urban watershed management under rapid urbanization
Pengfei DU, Haifeng JIA, Shaw L. YU
《环境科学与工程前沿(英文)》 2012年 第6卷 第5期 页码 595-595 doi: 10.1007/s11783-012-0452-5
Recombinant aspartate aminotransferase-catalyzed synthesis of L-4-fluorophenylalanine
CHEN Meijuan, JIA Honghua, CHEN Yongsheng, JIANG Min, WEI Ping
《化学科学与工程前沿(英文)》 2008年 第2卷 第2期 页码 220-223 doi: 10.1007/s11705-008-0041-0
关键词: L-4-Fluorophenylalanine Asp-AT aspartate aminotransferase 4-fluorophenylpyruvate FPPA
null
《医学前沿(英文)》 2017年 第11卷 第1期 页码 137-146 doi: 10.1007/s11684-016-0486-3
This study aims to elucidate the underlying molecular mechanisms of artemisinin accumulation induced by cadmium (Cd). The effects of different Cd concentrations (0, 20, 60, and 120 μmol/L) on the biosynthesis of Artemisia annua L. were examined. Intermediate and end products were quantified by HPLC-ESI-MS/MS analysis. The expression of key biosynthesis enzymes was also determined by qRT-PCR. The results showed that the application of treatment with 60 and 120 μmol/L Cd for 3 days significantly improved the biosynthesis of artemisinic acid, arteannuin B, and artemisinin. The concentrations of artemisinic acid, arteannuin B, and artemisinin in the 120 μmol/L Cd-treated group were 2.26, 102.08, and 33.63 times higher than those in the control group, respectively. The concentrations of arteannuin B and artemisinin in 60 μmol/L Cd-treated leaves were 61.10 and 26.40 times higher than those in the control group, respectively. The relative expression levels of HMGR, FPS, ADS, CYP71AV1, DBR2, ALDH1, and DXR were up-regulated in the 120 μmol/L Cd-treated group because of increased contents of artemisinic metabolites after 3 days of treatment. Hence, appropriate doses of Cd can increase the concentrations of artemisinic metabolites at a certain time point by up-regulating the relative expression levels of key enzyme genes involved in artemisinin biosynthesis.
关键词: Cd secondary metabolites gene expressions Artemisia annua L.
Reactive extraction for preparation of hydrogen peroxide under pressure
CHENG Yongxi, LI Hongtao, WANG Li, LÜ Shuxiang
《化学科学与工程前沿(英文)》 2008年 第2卷 第3期 页码 335-340 doi: 10.1007/s11705-008-0042-z
Raymond L. Sterling
《工程(英文)》 2017年 第3卷 第6期 页码 780-781 doi: 10.1016/j.eng.2017.12.006
Size-controlled green synthesis of silver nanoparticles assisted by L-cysteine
Wenchao Zhang,Lin Zhang,Yan Sun
《化学科学与工程前沿(英文)》 2015年 第9卷 第4期 页码 494-500 doi: 10.1007/s11705-015-1527-1
关键词: nanoparticles silver L-cysteine size distribution synthesis
Quality Function Deployment Implementation in Construction: A Systematic Literature Review
Cudney,William L. Gillis
《工程管理前沿(英文)》 2016年 第3卷 第3期 页码 224-230 doi: 10.15302/J-FEM-2016036
关键词: construction engineering management quality function deployment systematic review
标题 作者 时间 类型 操作
ROUTE DEVELOPMENT, ANTIVIRAL STUDIES, FIELD EVALUATION AND TOXICITY OF AN ANTIVIRAL PLANT PROTECTANT NK0238
期刊论文
ROUTE DEVELOPMENT, ANTIVIRAL STUDIES, FIELD EVALUATION AND TOXICITY OF AN ANTIVIRAL PLANT PROTECTANT NK0238
Wentao XU, Hao TIAN, Hongjian SONG, Yuxiu LIU, Yongqiang LI, Qingmin WANG
期刊论文
PD-1/PD-L1 blockade in cervical cancer: current studies and perspectives
Yumeng Wang, Guiling Li
期刊论文
Gridded usage inventories of chlordane in China
Qiang WANG, Lijuan ZHAO, Xuekun FANG, Jianhua XU, Yifan LI, Yehong SHI, Jianxin HU
期刊论文
Shear design of high strength concrete prestressed girders
Emad L. LABIB,Hemant B. DHONDE,Thomas T. C. HSU,Y. L. MO
期刊论文
Recombinant aspartate aminotransferase-catalyzed synthesis of L-4-fluorophenylalanine
CHEN Meijuan, JIA Honghua, CHEN Yongsheng, JIANG Min, WEI Ping
期刊论文
Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua L.
null
期刊论文
Reactive extraction for preparation of hydrogen peroxide under pressure
CHENG Yongxi, LI Hongtao, WANG Li, LÜ Shuxiang
期刊论文
Size-controlled green synthesis of silver nanoparticles assisted by L-cysteine
Wenchao Zhang,Lin Zhang,Yan Sun
期刊论文