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Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,
《医学前沿(英文)》 2010年 第4卷 第2期 页码 204-207 doi: 10.1007/s11684-010-0043-4
关键词: Angiostrongylus cantonensis Pomacea canaliculata multiplex polymerase chain reaction diagnosis pooling
null
《医学前沿(英文)》 2018年 第12卷 第2期 页码 196-205 doi: 10.1007/s11684-017-0560-5
We employed a multiplex polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CE) targeting six Clostridium difficile genes, including tpi, tcdA, tcdB, cdtA, cdtB, and a deletion in tcdC for simultaneous detection and characterization of toxigenic C. difficile directly from fecal specimens. The mPCR-CE had a limit of detection of 10 colony-forming units per reaction with no cross-reactions with other related bacterial genes. Clinical validation was performed on 354 consecutively collected stool specimens from patients with suspected C. difficile infection and 45 isolates. The results were compared with a reference standard combined with BD MAX Cdiff, real-time cell analysis assay (RTCA), and mPCR-CE. The toxigenic C. difficile species were detected in 36 isolates and 45 stool specimens by the mPCR-CE, which provided a positive rate of 20.3% (81/399). The mPCR-CE had a specificity of 97.2% and a sensitivity of 96.0%, which was higher than RTCA (x2 = 5.67, P = 0.017) but lower than BD MAX Cdiff (P = 0.245). Among the 45 strains, 44 (97.8%) were determined as non-ribotype 027 by the mPCR-CE, which was fully agreed with PCR ribotyping. Even though ribotypes 017 (n = 8, 17.8%), 001 (n = 6, 13.3%), and 012 (n = 7, 15.6%) were predominant in this region, ribotype 027 was an important genotype monitored routinely. The mPCR-CE provided an alternative diagnosis tool for the simultaneous detection of toxigenic C. difficile in stool and potentially differentiated between RT027 and non-RT027.
关键词: Clostridium difficile multiplex PCR capillary electrophoresis detection characterization
Arthrogryposis multiplex congenita: classification, diagnosis, perioperative care, and anesthesia
null
《医学前沿(英文)》 2017年 第11卷 第1期 页码 48-52 doi: 10.1007/s11684-017-0500-4
Arthrogryposis multiplex congenita (AMC) is a rare disorder characterized by non-progressive, multiple contractures. In addition to affected extremities, patients may also present microstomia, decreased temporomandibular joint mobility. Although the etiology of AMC is unclear, any factor that decreases fetal movement is responsible for AMC. Thus, accurate diagnosis and classification are crucial to the appropriate treatment of AMC. The development of ultrasound technology has enabled prenatal diagnosis. Very early treatment is favorable, and multidisciplinary treatment is necessary to improve the function of AMC patients. Most patients require surgery to release contracture and reconstruct joints. However, perioperative care is challenging, and difficult airway is the first concern of anesthesiologists. Postoperative pulmonary complications are common and regional anesthesia is recommended for postoperative analgesia. This review on AMC is intended for anesthesiologists. Thus, we discuss the treatment and perioperative management of patients undergoing surgery, as well as the diagnosis and classification of AMC.
关键词: arthrogryposis amyoplasia distal arthrogryposis anesthesia
Using pyrosequencing and quantitative PCR to analyze microbial communities
Husen ZHANG
《环境科学与工程前沿(英文)》 2011年 第5卷 第1期 页码 21-27 doi: 10.1007/s11783-011-0303-9
关键词: polymerase chain reaction (PCR) microbial communities pyrosequencing gut microbial fuel cell sludge
李现明,张玉林,李建田
《中国工程科学》 2007年 第9卷 第7期 页码 76-81
设计了一种用于聚合酶链式反应的低成本微反应器,其内部无微加热器、微传感器。针对这种低成本的微反应器,提出了一种温度测量与控制方案,其特点是将各反应器温度的集中控制与分散控制有机结合,能够同时完成上百只微反应器快速、准确的温度循环。重点阐述了温度传感器负荷效应的提取与补偿、动态误差补偿、微反应器温度间接测量误差评定、微反应器温度串级控制策略。
Erratum to: Using pyrosequencing and quantitative PCR to analyze microbial communities
Husen ZHANG
《环境科学与工程前沿(英文)》 2011年 第5卷 第3期 页码 488-488 doi: 10.1007/s11783-011-0342-2
Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles
Juan Wang, Bin Jia, Zexiong Xie, Yunxiang Li, Yingjin Yuan
《化学科学与工程前沿(英文)》 2018年 第12卷 第4期 页码 806-814 doi: 10.1007/s11705-018-1739-2
The synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE) system has been used to improve prodeoxyviolacein (PDV) production in haploid yeast containing chromosome synV. To rapidly and continuously generate genome diversification with the desired phenotype, the multiplex SCRaMbLE iterative cycle strategy has been developed for the screening of high PDV production strains. Whole-genome sequencing analysis reveals large duplications, deletions, and even the whole genome duplications. The deletion of YER151C is proved to be responsible for the increase. This study demonstrates that artificial DNA rearrangement can be used to accelerate microbial evolution and the production of biobased chemicals.
关键词: synthetic biology genome rearrangement prodeoxyviolacein SCRaMbLE Saccharomyces cerevisiae
Safety evaluation of microbial pesticide (HaNPV) based on PCR method
Miao Zhao, Shufei Li, Qinghong Zhou, Dianming Zhou, Ning He, Zhiyong Qian
《化学科学与工程前沿(英文)》 2019年 第13卷 第2期 页码 377-384 doi: 10.1007/s11705-018-1777-9
关键词: microbial pesticides HaNPV acute intravenous injection infectivity pathogenicity
Pingping Liu,Jia Lv,Cen Ma,Tianqi Zhang,Xiaowen Huang,Zhihui Yang,Lingling Zhang,Jingjie Hu,Shi Wang,Zhenmin Bao,
《工程(英文)》 2022年 第18卷 第11期 页码 259-259 doi: 10.1016/j.eng.2022.09.002
关键词: online THE AUTHORS.Published repertoire behalf Engineering September genotyping Academy HD-marker approach” Education Press Limited Company.
彭年才,李 磊,李 政,苗保刚,李 明,赵 垚,蒋庄德
《中国工程科学》 2013年 第15卷 第1期 页码 57-62
以生物检测与分子诊断技术发展为背景,总结了团队在医学诊断、疾控、食品安全、检疫检验、生物学医学科学研究等领域的分子诊断高端装备的研制情况,重点介绍了实时荧光定量聚合酶链式反应(PCR)分子诊断仪关键技术的研发及仪器工程化技术
基于抗原和聚合酶链式反应的组合策略对模拟社区内遏制COVID-19传播效果的定量分析 Article
黄蔷如, 孙艳侠, 贾萌萌, 张婷, 陈方圆, 姜明月, 王晴, 冯录召, 杨维中
《工程(英文)》 2023年 第28卷 第9期 页码 234-242 doi: 10.1016/j.eng.2023.01.004
贾贵玺,李洪凤,齐炜
《中国工程科学》 2004年 第6卷 第11期 页码 58-62
智能多路数据采集系统实现了多路温度采集与检测,利用仪用放大器AD620进行信号放大,采用LM331型电压-频率转换器进行电量转换,采用89S52单片机进行数据采集及存储并用Visual Basic 6.0实现单片机与PC机的通信;最后给出了智能多路数据采集系统在梅江小区综合办公楼生态能源示范系统的应用数据。
Kun DING,Xianghua WEN,Liang CHEN,Daishi HUANG,Fan FEI,Yuyang LI
《环境科学与工程前沿(英文)》 2014年 第8卷 第5期 页码 693-702 doi: 10.1007/s11783-014-0635-3
关键词: ammonia-oxidizing archaea ammonia-oxidizing bacteria quantitative PCR clone library plateau
基于多层网络随机块模型的多重社交关系量化方法 Science Letter
吴旻诚,李振,邵存祺,贺诗波
《信息与电子工程前沿(英文)》 2021年 第22卷 第11期 页码 1458-1462 doi: 10.1631/FITEE.2000617
关键词: 社交网络;多重网络;随机块模型
标题 作者 时间 类型 操作
A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host
Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,
期刊论文
Simultaneous detection and characterization of toxigenic:Clostridium difficile directly from clinical stool specimens
null
期刊论文
Arthrogryposis multiplex congenita: classification, diagnosis, perioperative care, and anesthesia
null
期刊论文
Erratum to: Using pyrosequencing and quantitative PCR to analyze microbial communities
Husen ZHANG
期刊论文
Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles
Juan Wang, Bin Jia, Zexiong Xie, Yunxiang Li, Yingjin Yuan
期刊论文
Safety evaluation of microbial pesticide (HaNPV) based on PCR method
Miao Zhao, Shufei Li, Qinghong Zhou, Dianming Zhou, Ning He, Zhiyong Qian
期刊论文
Erratum to “Targeted Genotyping of a Whole-Gene Repertoire by an Ultrahigh-Multiplex and Flexible HD-Marker
Pingping Liu,Jia Lv,Cen Ma,Tianqi Zhang,Xiaowen Huang,Zhihui Yang,Lingling Zhang,Jingjie Hu,Shi Wang,Zhenmin Bao,
期刊论文
Abundance and distribution of ammonia-oxidizing archaea in Tibetan and Yunnan plateau agricultural soils of China
Kun DING,Xianghua WEN,Liang CHEN,Daishi HUANG,Fan FEI,Yuyang LI
期刊论文