Isolation of a strain that degrades 1,2,4-trichlorobenzene and
characterization of its degradative plasmid
SONG Lei, WANG Hui, SHI Hanchang, HU Hongying
《环境科学与工程前沿（英文）》
2008年
第2卷
第1期
页码 69-72
doi:
10.1007/s11783-008-0019-7
摘要：
The genetic information encoding metabolic pathways for xenobiotic compounds in bacteria often resides on catabolic plasmids. The aim of the present work was to know the location of the genes for degrading 1,2,4-trichlorobenzen. In this paper a 1,2,4-trichlorobenzene-degrading strain THSL-1 was isolated from the soil of Tianjin Chemical Plant using 1,2,4-trichlorobenzene as the sole carbon source. The strain was identified as through morphologic survey and 16S rDNA sequence determination. A plasmid was discovered from strain THSL-1 by using the alkali lysis method. When the plasmid was transformed into . JM109 by the CaCl2 method, the transformant could grow using 1,2,4-trichlorobenzene as the sole carbon source and had the degradation function of 1,2,4-trichlorobenzene. Therefore, it could be deemed that the plasmid carried the degradative genes of 1,2,4-trichlorobenzene. The average size of the plasmid was finally determined to be 40.2 Kb using selectively three kinds of restricted inscribed enzymes (III, HI, and I) for single cutting and double cutting the plasmid pTHSL-1, respectively.
关键词：
degradation function
information
1
2
4-trichlorobenzen
metabolic
xenobiotic
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