High-expression of recombinant human concensus interferon-α by Pichia pastoris

2007, Volume 1, Issue 4

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Frontiers of Chemical Science and Engineering >> 2007, Volume 1, Issue 4 doi: 10.1007/s11705-007-0073-x

High-expression of recombinant human concensus interferon-α by Pichia pastoris

1.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2.Institute of Bioengineering, Academy of Military Medical Sciences, Beijing 100071, China

Available online: 2007-12-05

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Abstract

The present work focused on the high expression of recombinant human consensus interferon- (cIFN) by Pichia pastoris. The cycle of glycerol feeding, the strategy of methanol feeding and the optimum pH for protein induction were studied. The optimized strategies were a 4-h glycerol-feeding period, induction pH being kept at 5.0 and methanol concentration being kept under 5 g/L. The maximum dry cell weight, cIFN production and bioactivity obtained were 168, 1.24 g/L and 5.4×10 U/mL, respectively.

Keywords

bioactivity ; pastoris ; maximum ; induction ; optimized

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