Frontiers of Chemical Science and Engineering
>> 2012,
Volume 6,
Issue 2
doi:
10.1007/s11705-012-1277-2
RESEARCH ARTICLE
Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up derivative
1. Biological Faculty, M. V. Lomonosov Moscow State University, Moscow 119991, Russia; 2. Institute of Bioorganic Chemistry (RAS) Miklukho-Maklaya 16/10, Moscow 117992, Russia
Available online: 2012-06-05
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Abstract
Antifungal activity of recombinant medicinal leech destabilase-lysozyme (rec.Dest-Lys) was investigated by using fungi: , and , including yeasts and . Its antibacterial activity was investigated on gram-negative bacteria . These activities were assessed by radial agar diffusion assay, and scanning and transmission electron microscopy. Therefore, destabilase-lysozyme not only is endo-isopeptidase and lysozyme, but also has antifungal and antibacterial activities. Muramidase activity of rec.Dest-Lys disappeared after heat-treating at 90°C for 50 min without the loss of its antimicrobial activity. Furthermore we showed for the first time that the heated-up derivative of rec.Dest-Lys exhibited more potent activities against the above enumerated fungi and gram-negative bacteria than original protein.
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