A simple umbelliferone based fluorescent probe for the detection of nitroreductase

2018, Volume 12, Issue 2

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Frontiers of Chemical Science and Engineering >> 2018, Volume 12, Issue 2 doi: 10.1007/s11705-017-1697-0

A simple umbelliferone based fluorescent probe for the detection of nitroreductase

. Department of Chemistry, University of Bath, Bath, BA2 7AY, UK.. Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland

Accepted: 2018-01-15 Available online: 2018-05-09

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Abstract

A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR.

Keywords

fragmentation ; NCOU1 ; NTR ; nitroreductase ; nitrobenzyl-umbelliferone

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