Detection of Escherichia coli in wastewater based on enzyme immunoassay

2007, Volume 1, Issue 3

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Frontiers of Environmental Science & Engineering >> 2007, Volume 1, Issue 3 doi: 10.1007/s11783-007-0065-6

Detection of Escherichia coli in wastewater based on enzyme immunoassay

State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China;

Available online: 2007-09-05

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Abstract

This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA) for in drainage of wastewater treatment plants. Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horse radish peroxidase (HRP)- conjugate, and anti-HPR antibody and pretreatment of were studied. Those results showed that the linear range of detection for was 10 cfu/mL– 6 × 10 cfu/mL. Compared with conventional methods, it is a convenient and sensitive detection method with low cost.

Keywords

conventional ; sandwich ; pretreatment ; enzyme-linked immunosorbent ; detection

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