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immunohistochemistry 4

ALK fusion 1

LGR6 1

ciclosporin 1

colon adenocarcinoma 1

different 1

fluorescence in situ hybridization 1

intratumoral heterogeneity 1

next-generation sequencing 1

phosphatase of regenerating liver 1 1

phosphatase of regenerating liver 3 1

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reconstructed adenovirus 1

reverse transcription polymerase chain reaction 1

reversible inflammation 1

targeted therapy 1

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Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

Frontiers of Medicine 2023, Volume 17, Issue 3,   Pages 493-502 doi: 10.1007/s11684-022-0946-x

Abstract: Anaplastic lymphoma kinase (ALK) is the most common fusion gene involved in non-small cell lung cancer (NSCLC), and remarkable response has been achieved with the use of ALK tyrosine kinase inhibitors (ALK-TKIs). However, the clinical efficacy is highly variable. Pre-existing intratumoral heterogeneity (ITH) has been proven to contribute to the poor treatment response and the resistance to targeted therapies. In this work, we investigated whether the variant allele frequencies (VAFs) of ALK fusions can help assess ITH and predict targeted therapy efficacy. Through the application of next-generation sequencing (NGS), 7.2% (326/4548) of patients were detected to be ALK positive. On the basis of the adjusted VAF (adjVAF, VAF normalization for tumor purity) of four different threshold values (adjVAF < 50%, 40%, 30%, or 20%), the association of ALK subclonality with crizotinib efficacy was assessed. Nonetheless, no statistical association was observed between median progression-free survival (PFS) and ALK subclonality assessed by adjVAF, and a poor correlation of adjVAF with PFS was found among the 85 patients who received first-line crizotinib. Results suggest that the ALK VAF determined by hybrid capture-based NGS is probably unreliable for ITH assessment and targeted therapy efficacy prediction in NSCLC.

Keywords： ALK fusion     next-generation sequencing     fluorescence in situ hybridization     immunohistochemistry    

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Investigation of gene therapy of denovirus in immune suppression

XIA Xi, WANG Beibei, CAO Li, CHEN Gang, WU Peng, LU Yunping, ZHOU Jianfeng, MA Ding

Frontiers of Medicine 2008, Volume 2, Issue 4,   Pages 386-390 doi: 10.1007/s11684-008-0074-2

Abstract: Immunohistochemistry was used to examine the expression of adenovirus in organs.HE stained sections of the organs, some transitory and reversible inflammation was detected. (2) In immunohistochemistry

Keywords： different     reversible inflammation     immunohistochemistry     reconstructed adenovirus     ciclosporin    

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G protein-coupled receptor LGR6 is an independent risk factor for colon adenocarcinoma

Wenjing Wang, Shigang Ding, Hejun Zhang, Jun Li, Jun Zhan, Hongquan Zhang

Frontiers of Medicine 2019, Volume 13, Issue 4,   Pages 482-491 doi: 10.1007/s11684-018-0633-0

Abstract: Immunohistochemistry was used to detect LGR6 expression in colon adenoma tissues ( = 21), colon adenocarcinoma

Keywords： LGR6     colon adenocarcinoma     immunohistochemistry     prognosis    

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Expression and clinical implication of PRL-1 and PRL-3 in transitional cell carcinoma of bladder

Bin HAO, Changwei LIU, Huixiang LI

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 197-203 doi: 10.1007/s11684-009-0036-3

Abstract: and 10 cases of normal bladder, and the expression of PRL-1 and PRL-3 protein was checked by using immunohistochemistry

Keywords： regenerating liver 1     phosphatase of regenerating liver 3     reverse transcription polymerase chain reaction     immunohistochemistry    

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Title Author Date Type Operation

Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

Journal Article

Investigation of gene therapy of denovirus in immune suppression

XIA Xi, WANG Beibei, CAO Li, CHEN Gang, WU Peng, LU Yunping, ZHOU Jianfeng, MA Ding

Journal Article

G protein-coupled receptor LGR6 is an independent risk factor for colon adenocarcinoma

Wenjing Wang, Shigang Ding, Hejun Zhang, Jun Li, Jun Zhan, Hongquan Zhang

Journal Article

Expression and clinical implication of PRL-1 and PRL-3 in transitional cell carcinoma of bladder

Bin HAO, Changwei LIU, Huixiang LI

Journal Article