电针刺激促进 Mecp2基因敲除的Rett综合征模型大鼠功能恢复

工程（英文） ›› 2022, Vol. 12 ›› Issue (5) : 83 -89. DOI: 10.1016/j.eng.2020.06.032

研究论文

孙艳红 ^a^,^b^,^* ,
陈枝芳 ^c^,^* ,
徐毅 ^d^,^* ,
张月芳 ^c ,
葛志磊 ^e ,
林承列 ^f ,
周宜 ^f ,
赵芳菲 ^b ,
闫美玲 ^b ,
刘馨怡 ^b ,
诸颖 ^a^,^b ,
高基民 ^d ,
李宏义 ^g ,
王丽华 ^a^,^b ,
胡钧 ^a^,^b ,
仇子龙 ^c ,
樊春海 ^e

作者信息 +

Functional Recovery with Electro-Acupuncture Stimulation in an Mecp2-Knockout Rat Model of Rett Syndrome

Yanhong Sun, Zhifang Chen, Yi Xu, Yuefang Zhang, Zhilei Ge, Chenglie Lin, Yi Zhou,Fangfei Zhao, Meiling Yan, Xinyi Liu, Ying Zhu, Jimin Gao, Hongyi Li, Lihua Wang, Jun Hu,Zilong Qiu, Chunhai Fan ^a^,^b^,^* ,

Author information +

文章历史 +

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摘要

Rett 综合征（又称雷特综合征）是一种进行性神经发育障碍性疾病，目前尚缺乏有效的治疗手段。已有研究表明，深部脑刺激可以缓解Rett 综合征模型小鼠的一些症状，但这种介入操作需要精细的手术。本研究采用电针刺激（EAS）百会（GV 20）、涌泉（KI 1）和神门（HT 7）穴位，可以减轻Mecp2 基因敲除的Rett 综合征模型大鼠的症状。结果表明，电针不仅延长了Rett 综合征大鼠的生存期，而且提高了其行为能力，包括运动能力、运动协调能力和社会交往能力。进一步研究发现，野生型和Rett 模型大鼠经电针刺激后，中脑黑质、纹状体和大脑皮层中c-Fos 蛋白表达的增加，表明电针刺激能激活这些大鼠脑区神经元的活动，从而促进运动及社交能力的恢复。因此，电针为神经发育性疾病治疗提供了一种安全有效的潜在治疗手段。

Abstract

Rett syndrome is a progressive neurodevelopmental disorder that lacks effective treatments. Although deep-brain stimulation can alleviate some symptoms in Rett model mice, this interventional manipulation requires deliberate surgical operations. Here, we report that electro-acupuncture stimulation (EAS) can ameliorate symptoms of an Mecp2-knockout rat model of Rett syndrome from the remote acupoints Baihui (GV 20), Yongquan (KI 1), and Shenmen (HT 7). We find that EAS not only prolongs the survival time of Rett rats, but also improves their behavior ability, including locomotion, motor coordination, and social interaction. Neural activation was observed in the substantia nigra of the midbrain, corpus striatum, and cerebral cortex of wild-type and Rett model rats, as reflected by the increased expression of the c-Fos protein. Hence, EAS provides a potential promising therapeutic tool for treating neurodevelopmental diseases.

关键词

神经发育障碍 / 电针 / Rett综合征 / 运动功能 / 社交

Key words

Neurodevelopmental disorder / Electro-acupuncture stimulation / Rett syndrome / Motor function / Social interaction

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孙艳红,陈枝芳,徐毅,张月芳,葛志磊,林承列,周宜,赵芳菲,闫美玲,刘馨怡,诸颖,高基民,李宏义,王丽华,胡钧,仇子龙,樊春海. 电针刺激促进 Mecp2基因敲除的Rett综合征模型大鼠功能恢复[J]. 工程（英文）, 2022, 12(5): 83-89 DOI:10.1016/j.eng.2020.06.032