工程化DNA材料构建DNA活字系统实现可持续的数据存储

巩子祎, 宋理富, 裴广胜, 董雨菲, 李炳志, 元英进

工程（英文） ›› 2023, Vol. 29 ›› Issue (10) : 130-136.

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工程（英文） ›› 2023, Vol. 29 ›› Issue (10) : 130-136. DOI: 10.1016/j.eng.2022.05.023
研究论文
Article

工程化DNA材料构建DNA活字系统实现可持续的数据存储

作者信息 +

Engineering DNA Materials for Sustainable Data Storage Using a DNA Movable-Type System

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History +

摘要

DNA分子作为一种具有潜力的数据存储绿色材料，具有密度高和保存期长的优势。然而，目前DNA数据存储的数据写入依赖于DNA从头合成，写入成本高昂，且产生有害物，限制了其实际应用。在本研究中，我们开发了一种DNA活字存储系统，该系统可以利用由细胞工厂预生产的DNA活字片段进行数据写入。在这个系统中，这些预先生成的DNA片段，在这里称为“DNA活字”，是可重复使用的基本数据单元。通过这些DNA活字的快速组装来实现数据写入，从而避免了昂贵且对环境有害的DNA化学合成过程。通过这个系统，我们成功地将24字节的数字信息编码到DNA中，并通过高通量测序和解码实现了准确读取，这证明了该系统的可行性。通过DNA活字片段的反复使用和生物组装，该系统在降低写入成本方面的潜力非常突出，为经济和可持续的DNA数据存储技术开辟了一条新颖路线。

Abstract

DNA molecules are green materials with great potential for high-density and long-term data storage. However, the current data-writing process of DNA data storage via DNA synthesis suffers from high costs and the production of hazards, limiting its practical applications. Here, we developed a DNA movable-type storage system that can utilize DNA fragments pre-produced by cell factories for data writing. In this system, these pre-generated DNA fragments, referred to herein as “DNA movable types,” are used as basic writing units in a repetitive way. The process of data writing is achieved by the rapid assembly of these DNA movable types, thereby avoiding the costly and environmentally hazardous process of de novo DNA synthesis. With this system, we successfully encoded 24 bytes of digital information in DNA and read it back accurately by means of high-throughput sequencing and decoding, thereby demonstrating the feasibility of this system. Through its repetitive usage and biological assembly of DNA movable-type fragments, this system exhibits excellent potential for writing cost reduction, opening up a novel route toward an economical and sustainable digital data-storage technology.

关键词

合成生物学 / DNA信息存储 / DNA活字存储系统 / 经济性DNA数据存储

Keywords

Synthetic biology / DNA data storage / DNA movable types / Economical DNA data storage

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巩子祎, 宋理富, 裴广胜. 工程化DNA材料构建DNA活字系统实现可持续的数据存储. Engineering. 2023, 29(10): 130-136 https://doi.org/10.1016/j.eng.2022.05.023

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This work was supported by the National Key Research and Development Program of China (2018YFA0900100), the Natural Science Foundation of Tianjin, China (19JCJQJC63300) and Tianjin University.

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