流感和COVID-19患者之间免疫球蛋白 G 糖基化的差异

Engineering ›› 2023, Vol. 26 ›› Issue (7) : 54 -62. DOI: 10.1016/j.eng.2022.08.007

研究论文

Marina Kljaković-Gašpić Batinjan ^a^,^* ,
Tea Petrović ^b^,^* ,
Frano Vučković ^b ,
Irzal Hadžibegović ^c^,^d ,
Barbara Radovani ^e ,
Ivana Jurin ^c ,
Lovorka Đerek ^f ,
Eva Huljev ^g ,
Alemka Markotić ^h^,ⁱ^,^j ,
Ivica Lukšić ^k ,
Irena Trbojević-Akmačić ^b ,
Gordan Lauc ^b^,^l ,
Ivan Gudelj ^b^,^e ,
Rok Čivljak ^g^,^m

作者信息 +

Differences in Immunoglobulin G Glycosylation Between Influenza and COVID-19 Patients

Marina Kljaković-Gašpić Batinjan ^a^,^* ,
Tea Petrović ^b^,^* ,
Frano Vučković ^b ,
Irzal Hadžibegović ^c^,^d ,
Barbara Radovani ^e ,
Ivana Jurin ^c ,
Lovorka Đerek ^f ,
Eva Huljev ^g ,
Alemka Markotić ^h^,ⁱ^,^j ,
Ivica Lukšić ^k ,
Irena Trbojević-Akmačić ^b ,
Gordan Lauc ^b^,^l ,
Ivan Gudelj ^b^,^e ,
Rok Čivljak ^g^,^m

Author information +

^a University Hospital Centre Zagreb, Zagreb 10000, Croatia

^b Genos Glycoscience Research Laboratory, Zagreb 10000, Croatia

^c Department of Cardiology, University Hospital Dubrava, Zagreb 10000, Croatia

^d Faculty of Dental Medicine and Health, Josip Juraj Strossmayer University, Osijek 31000, Croatia

^e Department of Biotechnology, University of Rijeka, Rijeka 51000, Croatia

^f Department for Laboratory Diagnostics, University Hospital Dubrava, Zagreb 10000, Croatia

^g Department for Acute Respiratory Infections, University Hospital for Infectious Diseases “Dr. Fran Mihaljević”, Zagreb 10000, Croatia

^h Department for Urogenital Infections, University Hospital for Infectious Diseases “Dr. Fran Mihaljević”, Zagreb 10000, Croatia

ⁱ Department for Infectious Diseases, School of Medicine, Catholic University of Croatia, 10000 Zagreb, Croatia

^j Postdoctoral Study, Faculty of Medicine, University of Rijeka, Rijeka 51000, Croatia

^k Department of Maxillofacial Surgery, University of Zagreb School of Medicine, Dubrava University Hospital, Zagreb 10000, Croatia

^l Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb 10000, Croatia

^m Department of Infectious Diseases, University of Zagreb School of Medicine, Zagreb 10000, Croatia

^* These authors contributed equally to this work.

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Abstract

The essential role of immunoglobulin G (IgG) in immune system regulation and combatting infectious diseases cannot be fully recognized without an understanding of the changes in its N-glycans attached to the asparagine 297 of the Fc domain that occur under such circumstances. These glycans impact the antibody stability, half-life, secretion, immunogenicity, and effector functions. Therefore, in this study, we analyzed and compared the total IgG glycome—at the level of individual glycan structures and derived glycosylation traits (sialylation, galactosylation, fucosylation, and bisecting N-acetylglucosamine (GlcNAc))—of 64 patients with influenza, 77 patients with coronavirus disease 2019 (COVID-19), and 56 healthy controls. Our study revealed a significant decrease in IgG galactosylation, sialylation, and bisecting GlcNAc (where the latter shows the most significant decrease) in deceased COVID-19 patients, whereas IgG fucosylation was increased. On the other hand, IgG galactosylation remained stable in influenza patients and COVID-19 survivors. IgG glycosylation in influenza patients was more time-dependent: In the first seven days of the disease, sialylation increased and fucosylation and bisecting GlcNAc decreased; in the next 21 days, sialylation decreased and fucosylation increased (while bisecting GlcNAc remained stable). The similarity of IgG glycosylation changes in COVID-19 survivors and influenza patients may be the consequence of an adequate immune response to enveloped viruses, while the observed changes in deceased COVID-19 patients may indicate its deviation.

关键词

流感 / 新冠病毒肺炎 / 病毒感染 / 糖基化 / 免疫球蛋白G / 肺炎

Key words

Influenza / COVID-19 / Viral infection / Glycosylation / Immunoglobulin G / Pneumonia

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Marina Kljaković-Gašpić Batinjan,Tea Petrović,Frano Vučković,Irzal Hadžibegović,Barbara Radovani,Ivana Jurin,Lovorka Đerek,Eva Huljev,Alemka Markotić,Ivica Lukšić,Irena Trbojević-Akmačić,Gordan Lauc,Ivan Gudelj,Rok Čivljak. 流感和COVID-19患者之间免疫球蛋白 G 糖基化的差异[J]. 工程（英文）, 2023, 26(7): 54-62 DOI:10.1016/j.eng.2022.08.007