短波红外荧光成像活体监测和评估植入的M2巨噬细胞在骨骼肌再生中的作用

Mo Chen; Yuzhou Chen; Sijia Feng; Shixian Dong; Luyi Sun; Huizhu Li; Fuchun Chen; Nguyen Thi Kim Thanh; Yunxia Li; Shiyi Chen; You Wang; Jun Chen

doi:10.1016/j.eng.2023.05.010

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工程（英文） ›› 2024, Vol. 33 ›› Issue (2) : 283-294. DOI: 10.1016/j.eng.2023.05.010

研究论文

Article

短波红外荧光成像活体监测和评估植入的M2巨噬细胞在骨骼肌再生中的作用

Mo Chen ^a^,^b ,
Yuzhou Chen ^a ,
Sijia Feng ^a ,
Shixian Dong ^c ,
Luyi Sun ^a ,
Huizhu Li ^a ,
Fuchun Chen ^d ,
Nguyen Thi Kim Thanh ^e^,^f ,
Yunxia Li ^a ,
Shiyi Chen ^a ,
You Wang ^b^,^* ,
Jun Chen ^a^,^*

作者信息 +

SWIR Fluorescence Imaging In Vivo Monitoring and Evaluating Implanted M2 Macrophages in Skeletal Muscle Regeneration

Mo Chen ^a^,^b^,^# ,
Yuzhou Chen ^a^,^# ,
Sijia Feng ^a^,^# ,
Shixian Dong ^c ,
Luyi Sun ^a ,
Huizhu Li ^a ,
Fuchun Chen ^d ,
Nguyen Thi Kim Thanh ^e^,^f ,
Yunxia Li ^a ,
Shiyi Chen ^a ,
You Wang ^b^,^* ,
Jun Chen ^a^,^*

Author information +

History +

Abstract

Skeletal muscle has a robust regeneration ability that is impaired by severe injury, disease, and aging, resulting in a decline in skeletal muscle function. Therefore, improving skeletal muscle regeneration is a key challenge in treating skeletal muscle-related disorders. Owing to their significant role in tissue regeneration, implantation of M2 macrophages (M2Mø) has great potential for improving skeletal muscle regeneration. Here, we present a short-wave infrared (SWIR) fluorescence imaging technique to obtain more in vivo information for an in-depth evaluation of the skeletal muscle regeneration effect after M2Mø transplantation. SWIR fluorescence imaging was employed to track implanted M2Mø in the injured skeletal muscle of mouse models. It is found that the implanted M2Mø accumulated at the injury site for two weeks. Then, SWIR fluorescence imaging of blood vessels showed that M2Mø implantation could improve the relative perfusion ratio on day 5 (1.09 ± 0.09 vs 0.85 ± 0.05; p = 0.01) and day 9 (1.38 ± 0.16 vs 0.95 ± 0.03; p = 0.01) post-injury, as well as augment the degree of skeletal muscle regeneration on day 13 post-injury. Finally, multiple linear regression analyses determined that post-injury time and relative perfusion ratio could be used as predictive indicators to evaluate skeletal muscle regeneration. These results provide more in vivo details about M2Mø in skeletal muscle regeneration and confirm that M2Mø could promote angiogenesis and improve the degree of skeletal muscle repair, which will guide the research and development of M2Mø implantation to improve skeletal muscle regeneration.

Keywords

In vivo / Short-wave infrared / Skeletal muscle / Macrophage / Regeneration

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Mo Chen, Yuzhou Chen, Sijia Feng. . Engineering. 2024, 33(2): 283-294 https://doi.org/10.1016/j.eng.2023.05.010

参考文献

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