通过机器学习开发基于作物基因型的抗病表型精准预测方法

刘琪, 左世敏, 彭沙莎, 张昊, 彭烨, 李魏, 熊叶辉, 林润茂, 冯志明, 李慧慧, 杨俊, 王国梁, 康厚祥

工程（英文） ›› 2024, Vol. 40 ›› Issue (9) : 100-110.

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工程（英文） ›› 2024, Vol. 40 ›› Issue (9) : 100-110. DOI: 10.1016/j.eng.2024.03.014
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通过机器学习开发基于作物基因型的抗病表型精准预测方法

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Development of Machine Learning Methods for Accurate Prediction of Plant Disease Resistance

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Abstract

The traditional method of screening plants for disease resistance phenotype is both time-consuming and costly. Genomic selection offers a potential solution to improve efficiency, but accurately predicting plant disease resistance remains a challenge. In this study, we evaluated eight different machine learning (ML) methods, including random forest classification (RFC), support vector classifier (SVC), light gradient boosting machine (lightGBM), random forest classification plus kinship (RFC_K), support vector classification plus kinship (SVC_K), light gradient boosting machine plus kinship (lightGBM_K), deep neural network genomic prediction (DNNGP), and densely connected convolutional networks (DenseNet), for predicting plant disease resistance. Our results demonstrate that the three plus kinship (K) methods developed in this study achieved high prediction accuracy. Specifically, these methods achieved accuracies of up to 95% for rice blast (RB), 85% for rice black-streaked dwarf virus (RBSDV), and 85% for rice sheath blight (RSB) when trained and applied to the rice diversity panel I (RDPI). Furthermore, the plus K models performed well in predicting wheat blast (WB) and wheat stripe rust (WSR) diseases, with mean accuracies of up to 90% and 93%, respectively. To assess the generalizability of our models, we applied the trained plus K methods to predict RB disease resistance in an independent population, rice diversity panel II (RDPII). Concurrently, we evaluated the RB resistance of RDPII cultivars using spray inoculation. Comparing the predictions with the spray inoculation results, we found that the accuracy of the plus K methods reached 91%. These findings highlight the effectiveness of the plus K methods (RFC_K, SVC_K, and lightGBM_K) in accurately predicting plant disease resistance for RB, RBSDV, RSB, WB, and WSR. The methods developed in this study not only provide valuable strategies for predicting disease resistance, but also pave the way for using machine learning to streamline genome-based crop breeding.

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Predicting plant disease resistance / Genomic selection / Machine learning / Genome-wide association study

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刘琪, 左世敏, 彭沙莎. 通过机器学习开发基于作物基因型的抗病表型精准预测方法. Engineering. 2024, 40(9): 100-110 https://doi.org/10.1016/j.eng.2024.03.014

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