The designing and manufacturing of micro/nanoscale tools for delivery, diagnostic, and therapeutic are essential for their multiscale integration in the precision medicine field. Conventional three-dimensional (3D) printing approaches are not suitable for such kind of tools due to the accuracy limitation. Multiphoton polymerization (MPP)-based micro/nanomanufacturing is a noncontact, high-precision molding technology that has been widely used in the micro/nano field is a promising tool for micro/nanoscale related precision medicine. In this article the fundamentals of MPP-based technology and the required materials in precision medicine are overviewed. The biomedical applications in various scenarios are then summarized and categorized as delivery systems, microtissue modeling, surgery, and diagnosis. Finally, the existing challenges and future perspectives on MPP-based micro/nanomanufacturing for precision medicine are discussed, focusing on material design, process optimization, and practical applications to overcome its current limitations.