Spray-drying is a widely used industrial technique to achieve the scale-up fabrication of functional powders. In this work, we report the spray-drying fabrication of perovskite quantum dot (PQD) microspheres from a precursor solution at a scale of 2000 kg∙a⁻¹. The obtained PQDs are embedded in polymer microspheres, resulting in a high photoluminescence quantum yield and enhanced stability. By controlling the precursor concentration, the average size of the polymer microspheres can be tuned from 40.97 to 0.44 μm. The as-prepared PQD-embedded polymer microspheres are mixed with ultraviolet adhesive to fabricate PQD-enhanced optical films for liquid crystal display (LCD) backlights. These films exhibit long-term operational stability under heat, humidity, and blue light irradiation (remaining at more than 90% initial photoluminescence intensity after a 1000 h aging test at 60 °C with 90% relative humidity and 70 °C with 455 nm 150 W∙m⁻² blue light irradiation). In addition, we demonstrate the use of PQD-embedded polymer microspheres as patterned color converters for micro light-emitting diode applications. Overall, this work demonstrates the scale-up fabrication of PQDs toward industrialization in display technology.