面向卵巢癌研究的多细胞肿瘤芯片模型构建

Jiexian Ye; Hao Lin; Zilin Zhang; Shihui Xu; Feili Yang; Xuemei Zhuansun; Feng Ji; Yusha Zhang; Yuxin Zhu; Jing Zhang; Zaozao Chen; Zhongze Gu; Yang Shen

doi:10.1016/j.eng.2025.08.028

工程（英文） ›› 2026, Vol. 62 ›› Issue (7) : 130 -142. DOI: 10.1016/j.eng.2025.08.028

研究论文

面向卵巢癌研究的多细胞肿瘤芯片模型构建

# These authors contributed equally to this work.

Jiexian Ye ^a^,^c^,^# ,
Hao Lin ^b^,^# ,
Zilin Zhang ^a ,
Shihui Xu ^a^,^c^,^d ,
Feili Yang ^c ,
Xuemei Zhuansun ^c ,
Feng Ji ^b ,
Yusha Zhang ^e ,
Yuxin Zhu ^e ,
Jing Zhang ^c ,
Zaozao Chen ^a^,^c^,^* ,
Zhongze Gu ^a^,^c^,^* ,
Yang Shen ^e^,^*

作者信息 +

Construction of Multicellular Tumor-On-A-Chip Models for Ovarian Cancer Research

Jiexian Ye ^a^,^c^,^# ,
Hao Lin ^b^,^# ,
Zilin Zhang ^a ,
Shihui Xu ^a^,^c^,^d ,
Feili Yang ^c ,
Xuemei Zhuansun ^c ,
Feng Ji ^b ,
Yusha Zhang ^e ,
Yuxin Zhu ^e ,
Jing Zhang ^c ,
Zaozao Chen ^a^,^c^,^* ,
Zhongze Gu ^a^,^c^,^* ,
Yang Shen ^e^,^*

Author information +

文章历史 +

Abstract

Ovarian cancer remains a highly lethal gynecologic malignancy. Early diagnosis poses significant challenges, and the five-year survival rate is consistently below 45 %. Current standard-of-care combines surgical resection with platinum-based chemotherapy. Emerging therapeutic modalities like chimeric antigen receptor-T (CAR-T) therapy show promise, though they face efficacy constraints due to tumor heterogeneity and immunosuppressive microenvironments. Conventional models including two-dimensional (2D) cultures and patient-derived xenografts are increasingly supplanted by organoid and tumor-on-a-chip technologies due to intrinsic limitations and poor clinical translatability. This study established multiple tumor-on-a-chip platforms derived from ovarian cancer organoids and conducted systematic in vitro drug sensitivity screening. Furthermore, by utilizing patient-derived organoids to engineer multicellular dynamic microenvironments, we achieved one of the extremely limited evaluations of CAR-T efficacy against solid tumors within ovarian cancer microfluidic systems. This work establishes an enhanced preclinical platform to advance therapeutic screening and personalized treatment development.

关键词

Key words

Ovarian cancer / Chimeric antigen receptor-mesothelin-T / Organoid / Tumor-on-a-chip / Tumor microenvironment

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引用格式 ▾

Jiexian Ye,Hao Lin,Zilin Zhang,Shihui Xu,Feili Yang,Xuemei Zhuansun,Feng Ji,Yusha Zhang,Yuxin Zhu,Jing Zhang,Zaozao Chen,Zhongze Gu,Yang Shen. 面向卵巢癌研究的多细胞肿瘤芯片模型构建[J]. 工程（英文）, 2026, 62(7): 130-142 DOI:10.1016/j.eng.2025.08.028