利用微流控技术在水凝胶微球上构建胶质瘤微模型以用于药物测试

Yingrui Zhang; Zengnan Wu; Jingyang Li; Shiyu Chen; Tong Xu; Shulang Chen; Xiaorui Wang; Yanli Guo; Yi Zhang; Jin-Ming Lin

doi:10.1016/j.eng.2026.01.010

工程（英文） ›› 2026, Vol. 61 ›› Issue (6) : 421 -429. DOI: 10.1016/j.eng.2026.01.010

研究论文

利用微流控技术在水凝胶微球上构建胶质瘤微模型以用于药物测试

Yingrui Zhang ^a ,
Zengnan Wu ^b^,^* ,
Jingyang Li ^a ,
Shiyu Chen ^a ,
Tong Xu ^a ,
Shulang Chen ^b ,
Xiaorui Wang ^b ,
Yanli Guo ^c ,
Yi Zhang ^a^,^d^,^* ,
Jin-Ming Lin ^b^,^*

作者信息 +

Microfluidic Construction of Glioma Micromodels on Hydrogel Microspheres for Drug Testing

Yingrui Zhang ^a ,
Zengnan Wu ^b^,^* ,
Jingyang Li ^a ,
Shiyu Chen ^a ,
Tong Xu ^a ,
Shulang Chen ^b ,
Xiaorui Wang ^b ,
Yanli Guo ^c ,
Yi Zhang ^a^,^d^,^* ,
Jin-Ming Lin ^b^,^*

Author information +

文章历史 +

Abstract

Gliomas are aggressive brain tumors associated with poor prognosis, necessitating advanced in vitro models for efficient drug screening. In this study, we develop a microfluidic glioma-on-a-microsphere model based on compartmentalized hydrogel microspheres with engineered rough surfaces. Each microsphere enables to accurately structure U251 glioma cells, M2-polarized THP-1 macrophages, and endothelial cells that can form a functional endothelial barrier on the Matrigel-modified microsphere surface. The model exhibited high cell viability, controllable molecular permeability, and upregulated expression of glioma-associated genes compared to conventional two-dimensional (2D) cultures. To validate the practicality of this platform in drug evaluation, chlorogenic acid was applied, resulting in suppressed gene expression, repolarization of macrophages toward an M1 phenotype, and significant alterations in key metabolites including tryptophan, glutamate, and lactate. Overall, this hydrogel microsphere-based glioma model offers a high-throughput and physiologically relevant platform for drug screening, disease modeling, and personalized therapeutic development.

关键词

Key words

Microfluidic / Glioma-on-a-microsphere / Hydrogel microsphere / Glioma / Drug testing

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Yingrui Zhang,Zengnan Wu,Jingyang Li,Shiyu Chen,Tong Xu,Shulang Chen,Xiaorui Wang,Yanli Guo,Yi Zhang,Jin-Ming Lin. 利用微流控技术在水凝胶微球上构建胶质瘤微模型以用于药物测试[J]. 工程（英文）, 2026, 61(6): 421-429 DOI:10.1016/j.eng.2026.01.010