应用实时定量聚合酶链式反应（PCR）技术检测子宫肌瘤组织中的hsa-miR-197 表达水平，并与配对正常子宫肌细胞对比。通过miRbase、UCSC、NCBI等在线数据库获取并分析hsa-miR-197 序列保守性及其基因组特征。选择miRanda、MirTarget2 及TargetScan 三个在线数据库预测hsa-miR-197 的靶基因并取交集以便后续分析。通过基因本体（GO）功能注释、GO富集分析和信号转导通路富集分析初步阐明，hsamiR-197 靶基因参与调控的细胞功能与信号通路。hsa-miR-197 的功能较广泛，参与肿瘤发生的多种生物学过程，提示hsa-miR-197 可能与子宫肌瘤的发病机制密切相关。

We performed Real-time PCR to detect the hsa-miR-197 expression levels in uterine leiomyomas tissues and paired normal myometrium separately；online databases including miRbase，UCSC，NCBI are employed to analysis the sequence conservation and genetic characteristics of hsa- miR- 197；miRNA databases such as miRanda，MirTarget2 and TargetScan are chosen to predict hsa- miR- 197 target genes；meanwhile，the intersection genes of these miRNA databases are chosen，and GO and Pathway analysis of these genes are carried out to explore the potential role of hsa-miR-197 playing in regulating the uterine leiomyomas occurrence and development. hsa-miR-197 functions in broad ground and participates in uterine leiomyomas multiple biology progress，which indicates that hsa- miR- 197 could regulate uterine leiomyomas occurrence and development.

1979年出生，女，浙江上虞市人，主治医师，研究方向为妇科肿瘤