应用细胞工程技术研制大菱鲆免疫球蛋白M(immunoglobulin M，IgM)的单克隆抗体并分析其免疫学特性。小鼠骨髓瘤细胞NS0 与经IgM免疫的BALB/C小鼠脾细胞融合，经过反复有限稀释法克隆，筛选获得4 株抗大菱鲆IgM的单克隆抗体杂交瘤细胞株，分别为B1D1、D5C2、E1B2 和F4A1。经小鼠腹水扩大生产后，单抗效价为1∶1.024×10⁶检测灵敏度为32 ng/mL。Western blot 分析表明，获得的单抗与IgM 重链区特异性结合。交叉结果显示，单抗与大菱鲆血清呈强阳性反应，与褐牙鲆、红鳍东方鲀、许氏平鲉、六线鱼、鲈鱼均呈微弱阳性反应，而与半滑舌鳎、鲤鱼、鲫鱼、草鱼、鳙鱼的血清无交叉反应。本研究制备的大菱鲆IgM单克隆抗体效价高、灵敏度高、特异性强，适合用于大菱鲆免疫学相关研究和生产实际应用。

Four monoclonal antibodies (mAbs) to immunoglobulin M (IgM) of turbot Scophthalmus maximus were produced and characterized. All the mAbs (denominated B1D1, D5C2, E1B2 and F4A1) are showed good anti-turbot IgM reactivity in enzyme linked immunosorbent assay (ELISA) and immunoblotting. Results of ELISA indicated that the titre of the mAb was 1:1.024×10⁶ and the minimum detectable amount of IgM was 32 ng/mL. Results of immunoblotting showed that the mAb reacted with heavy chain of IgM. The mAb showed specificity for Scophthalmus maximus, light specificity for Paralichthys olivaceus, Takifugu rubripes, Sebastodes fuscescens, Hexagrammos otakii, Lateolabrax japonicas, and no specificity for Cynoglossus semilaevis, Cyprinus carpio, Carassius cuvieri, Ctenopharyngodon idellus, Aristichthys nobilis. The mAbs characterization should prove useful for studying the biology of IgM and its practical application.

1980年出生，女，吉林通化市人，博士，助理研究员，研究方向为鱼类免疫学