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《工程(英文)》 >> 2017年 第3卷 第1期 doi: 10.1016/J.ENG.2017.01.001

一种新型生物材料——玉米醇溶蛋白多孔支架的质量监控

School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China

录用日期: 2017-01-23 发布日期: 2017-02-28

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摘要

我们之前的研究显示玉米醇溶蛋白具有良好的生物相容性和机械特性。由玉米醇溶蛋白多孔支架制成的第一个产品——一种可吸收的骨替代物,已经通过了国家食品药品监督管理总局医疗器械的生物学评价(ISO 10993)。然而,作为一种三类医疗器械,在其进入市场前需要进行质量监控,这些监控包括原材料的质量监控、灭菌方法的选择和生物相容性的评价。在本文中,我们通过氨基酸分析(AAA) 和十二烷基硫酸钠–聚丙烯酰氨凝胶电泳(SDS-PAGE) 研究了四种玉米醇溶蛋白,来监控其组成和纯度,以控制原材料的质量。我们用SDS-PAGE 研究了三种灭菌方式对玉米醇溶蛋白多孔支架的影响,并比较了在不同γ 射线剂量下SDS-PAGE 图谱的变化。我们发现在剂量20~30 kGy 范围内,γ 射线灭菌过程中玉米醇溶蛋白的肽链没有发生断裂或聚合,这表明γ 射线灭菌对于玉米醇溶蛋白多孔支架是适用的。对于细胞相容性,我们发现使用MTT 实验和CCK-8 实验来评估玉米醇溶蛋白膜上细胞的增殖是有区别的,由于在CCK-8 实验中背景的吸光度差异较小,我们认为CCK-8 实验更为适合。

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参考文献

[ 1 ] Gianazza E, Viglienghi V, Righetti PG, Salamini F, Soave C. Amino acid composition of zein molecular components. Phytochemistry 1977;16(3):315–7 链接1

[ 2 ] Shukal R, Munir C. Zein: the industrial protein from corn. Ind Crop Prod 2001;13(3):171–92 链接1

[ 3 ] Dong J, Sun Q, Wang J. Basic study of corn protein, zein, as a biomaterial in tissue engineering, surface morphology and biocompatibility. Biomaterials 2004;25(19):4691–7 链接1

[ 4 ] Gong S, Wang H, Sun Q, Xue S, Wang J. Mechanical properties and in vitro biocompatibility of porous zein scaffolds. Biomaterials 2006;27(20):3793–9 链接1

[ 5 ] Wang H, Gong S, Lin Z, Fu J, Xue S, Huang J, et al. In vivo biocompatibility and mechanical properties of porous zein scaffolds. Biomaterials 2007;28(27):3952–64 链接1

[ 6 ] Wang J, Wang H, Gong S. Preparation of zein scaffold for tissue engineering. In: Proceedings of the 8th World Biomaterials Congress ; 2008 May 28–Jun 1; Amsterdam, the Netherlands. North Miami Beach: Curran Associates, Inc.; 2008. p. 4:1787.

[ 7 ] Phillip E Jr, Murthy NS, Bolikal D, Narayanan P, Kohn J, Lavelle L, et al. Ethylene oxide’s role as a reactive agent during sterilization: effects of polymer composition and device architecture. J Biomed Mater Res-A 2013;101(4):532–40 链接1

[ 8 ] Lee MH, Kim HL, Kim CH, Lee SH, Kim JK, Lee SJ,et al. Effects of low temperature hydrogen peroxide gas on sterilization and cytocompatibility of porous poly(D, L-lactic-co-glycolic acid) scaffolds. Surf Coat Tech 2008;202(22– 23):5762–7 链接1

[ 9 ] Faraj KA, Brouwer KM, Geutjes PJ, Versteeg EM, Wismans RG, Deprest JA, et al. The effect of ethylene oxide sterilisation, beta irradiation and gamma irradiation on collagen fibril-based scaffolds. Tissue Eng Regen Med 2011;8(5):460–70.

[10] Rnjak-Kovacina J, DesRochers TM, Burke KA, Kaplan DL. The effect of sterilization on silk fibroin biomaterial properties. Macromol Biosci 2015;15(6):861–74 链接1

[11] Zhang Z, Chen W. Plating densities, alpha-difluoromethylornithine effects and time dependence on the proliferation of IEC-6 cells. China Med J 2002;115(4):518–20 链接1

[12] Cetin Y, Bullerman LB. Evaluation of reduced toxicity of zearalenone by extrusion processing as measured by the MTT cell proliferation assay. J Agr Food Chem 2005;53(16):6558–63 链接1

[13] Bruggisser R, von Daeniken K, Jundt G, Schaffner W, Tullberg-Reinert H. Interference of plant extracts, phytoestrogens and antioxidants with the MTT tetrazolium assay. Planta Med 2002;68(5):445–8 链接1

[14] Fischer J, Prosenc MH, Wolff M, Hort N, Willumeit R, Feyerabend F. Interference of magnesium corrosion with tetrazolium-based cytotoxicity assays. Acta Biomater 2010;6(5):1813–23 链接1

[15] Tominaga H, Ishiyama M, Ohseto F, Sasamoto K, Hamamoto T, Suzuki K, et al. A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Anal Commun 1999;36(2):47–50 链接1

[16] Jia J, Zhao X. GB/T 5009.124–2003 Determination of amino acids in foods. Beijing: Standards Press of China. 2003 Aug 11. Chinese.

[17] Wang L, Huang Q, Wang J. Nanostructured polyaniline coating on ITO glass promotes the neurite outgrowth of PC 12 cells by electrical stimulation. Langmuir 2015;31(44):12315–22 链接1

[18] ISO 11137-1:2006 Sterilization of health care products—Radiation—Part 1: requirements for development, validation and routine control of a sterilization process for medical devices. Geneva: International Organization for Standardization; 2006 Apr 15.

[19] Han Y, Xu Q, Lu Z, Wang J. Preparation of transparent zein films for cell culture applications. Colloid Surface B 2014;120(8):55–62 链接1

[20] Peng F, Wu H. 620 nm red light promotes celluar viability and mRNA expression of collagen type-I in bone mesenchymal stem cells of rat. In: SOPO 2010: Proceedings of the Symposium on Photonics and Optoelectronic ; 2010 Jun 19–21; Chengdu, China. New Jersey: IEEE; 2010. p. 1–3.

[21] Yan T, Sun R, Li C, Tan B, Mao X, Ao N. Immobilization of type-I collagen and basic fibroblast growth factor (bFGF) onto poly (HEMA-co-MMA) hydrogel surface and its cytotoxicity study. J Mater Sci-Mater Med 2010;21(8):2425–33 链接1

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