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《工程(英文)》 >> 2022年 第19卷 第12期 doi: 10.1016/j.eng.2021.03.024

基于链置换环介导等温扩增的量子点纳米珠标记侧流免疫检测试纸条快速灵敏检测鼠伤寒沙门氏菌

a Guangdong Provincial Key Laboratory of Microbial Safety and Health & State Key Laboratory of Applied Microbiology Southern China & Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China
b State Key Laboratory of Food Science and Technology & National Engineering Research Center for Functional Foods & Synergetic Innovation Center of Food Safety & Joint International Research Laboratory on Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

# These authors contributed equally to this work.

收稿日期: 2020-09-29 修回日期: 2020-12-18 录用日期: 2021-03-11 发布日期: 2021-07-17

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摘要

快速、灵敏、即时检测致病菌对食品安全至关重要。在这项研究中,开发了一种新的量子点纳米珠标记的侧流免疫检测试纸条(QB-labelled LFIAS)结合链置换环介导的等温扩增(SD-LAMP)技术,用于定量检测鼠伤寒沙门氏菌(ST)。量子点纳米珠(QB)作为荧光报告材料,具有良好的检测效率。在LAMP中使用了可定制的链置换(SD)探针,以提高方法的特异性并防止副产物捕获。检测基于夹心免疫分析法。荧光条读取器测量测试(T)线和对照(C)线的荧光强度(FI)。条带的线性检测范围为每毫升102 ~ 108个菌落形成单位(CFU)。视觉检出限为103 CFU·mL−1,表明该系统的灵敏度是AuNP 标记试纸的10 倍。根据聚合酶链反应(PCR)和SD-LAMP的琼脂糖凝胶输出分析ST 特异性。在食品中检测到ST,回收率为85%~110%。该方法快速、简便,几乎不需要设备,适用于食品中的细菌检测和临床诊断。

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