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期刊论文 43

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MS-CETSA 1

三维含孔洞结构 1

中草药化学成分 1

临震信号 1

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印度洋8.7级与8.5级地震 1

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金川铜镍矿床；构造属性；龙首山地块；西域板块；找矿方向 1

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Analysis of two new degradation products of arsenic triglutathione in aqueous solution

Feng ZHAO, Yuchen CHEN, Bin QIAO, Jing WANG, Ping NA

《化学科学与工程前沿（英文）》 2012年 第6卷 第3期   页码 292-300 doi: 10.1007/s11705-012-1208-2

摘要： Inorganic arsenicals, including arsenite (As ) and arsenate (As ), are well-known human carcinogens. Recently, studies have indicated that arsenic triglutathione (As(GS) ) is unstable in an aqueous solution. The present study was designed to evaluate the degradation mechanism of As(GS) in an aqueous solution using high-performance liquid chromatography-electrospray ionisation mass spectrometry (HPLC-ESI-MS). Based on the fragments obtained from MS and MS , we identified two new compounds: one was an isomer of glutathione (GSH), and the other was a product from the cleavage of the glutamyl of oxidised glutathione (GSSG). The isomerization of GSH resulted in the loss of its function such as detoxification of many reactive metabolites. The formation of the two new compounds affected the ratio of GSH/GSSG, and thus may affect the antioxidant and detoxification of GSH/GSSG in mammalian cells.

关键词： arsenic triglutathione     glutathione     HPLC-ESI-MS    

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从天然产物创制新药的发展趋势

于德泉

《中国工程科学》 1999年 第1卷 第2期   页码 87-90

摘要：

文章展望了从天然产物中创制新药的发展趋势。单味中草药有效成分的研究重点将转向瞄准质控的中药复方化学成分研究。进入21世纪，中国天然药物研究要在国际上占有重要位置，突破口便是中药复方现代化，其关键之一便是化学（有效）成分研究。从中草药中发现有效成分或先导物必须采用化学成分与生物活性研究融为一体的机制，采用高通量筛选，目标就是寻找有效成分或先导物进而创制新药。长短结合，正确处理知识创新和新药开发的关系。知识创新须体现在创制新药的应用基础研究中，是产生巨大效益的理论基础；短程开发是知识累积水平的体现，是科研经费的必要补充和保证。改革传统天然产物化学研究模式，实施以当代最先进大型谱仪为先导的天然产物研究战略。

关键词： 天然产物     创制新药     中草药化学成分     波谱技术     高效色谱与质谱联用     高效色谱与核磁共振联用    

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Photocatalytic degradation of methyl orange using ZnO/TiO composites

Ming GE , Changsheng GUO , Xingwang ZHU , Lili MA , Wei HU , Yuqiu WANG , Zhenan HAN ,

《环境科学与工程前沿（英文）》 2009年 第3卷 第3期   页码 271-280 doi: 10.1007/s11783-009-0035-2

摘要： ZnO/TiO composites were synthesized by using the solvothermal method and ultrasonic precipitation followed by heat treatment in order to investigate their photocatalytic degradation of methyl orange (MO) in aqueous suspension under UV irradiation. The composition and surface structure of the catalyst were characterized by X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM), and transmission electron microscopy (TEM). The degradation efficiencies of MO at various pH values were obtained. The highest degradation efficiencies were obtained before 30min and after 60min at pH 11.0 and pH 2.0, respectively. A sample analysis was conducted using liquid chromatography coupled with electrospray ionization ion-trap mass spectrometry. Six intermediates were found during the photocatalytic degradation process of quinonoid MO. The degradation pathway of quinonoid MO was also proposed.

关键词： photocatalytic degradation     methyl orange     ZnO/TiO2 composites     high performance liquid chromatography mass spectrometry (HPLC-MS)    

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Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis

Yansong HOU, Wei LIANG, Liping ZHANG, Shuiping CHENG, Feng HE, Zhenbin WU

《环境科学与工程前沿（英文）》 2011年 第5卷 第1期   页码 84-91 doi: 10.1007/s11783-010-0283-1

摘要： The study of community composition of algae is essential for understanding the structure and dynamics of the aquatic ecosystem and for evaluating the eutrophic level of the water body. A high-performance liquid chromatographic (HPLC) method based on a reverse-phase C nonpolar column was developed for the main algal taxa, which includes cyanophytes, bacillariophytes, euglenophytes, dinophytes, and chlorophytes. Based on the elution order using HPLC, 19 pigments were identified, and they were chlorophyllide , 19′-butanoyloxyfucoxanthin, chlorophyll + , phephorbides , peridinin, methyl-chlorophyllide , fucoxanthin, neoxanthin, violaxanthin, myxoxanthophyll, diadinoxanthin, diatoxanthin, lutein, zeaxanthin, chlorophyll allomer, chlorophyll , chlorophyll allomer, chlorophyll , and β,β-carotene. A comparison study of cell microscopic counts and accessory pigment analysis indicated that HPLC analysis could be a useful tool for monitoring phytoplankton communities and their abundance.

关键词： high-performance liquid chromatographic (HPLC)     algae     pigment     chemotaxonomy    

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Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAOMS,

《医学前沿（英文）》 2010年 第4卷 第2期   页码 216-219 doi: 10.1007/s11684-010-0037-2

摘要： To acquire data of Brucella cellular fatty acids (CFAs) and probe into the possibility of utilizing CFAs information in typing Brucella, 19 reference strains were subjected to CFAs study. After all strains were inoculated on Brucella Agar plates, the cells were harvested, saponificated, methylated and extracted to provide fatty acid methylesters for gas chromatography (GC) analysis. Based on the CFAs data matrix, a dendrogram of 19 reference strains was generated by SPSS11.5 software package. The results showed that 19 reference strains were divided into five clusters: cluster 1 included (bv. 1, 2, 3, 5) and ; cluster 2 included (bv. 3, 4, 5, 6) and (bv. 1, 2, 3); cluster 3 included (bv. 1, 2, 7, 9) and ; cluster 4 was (bv. 4); and cluster 5 was . Typing Brucella by GC analysis of CFAs is a good method to reflect drug resistance of Brucella, and the classification is beneficial for clinical therapy. It also provides a new result of typing and demonstrates that the traditional classification is not completely reasonable. CFAs analysis may identify (bv. 4) and .

关键词： Brucella     gas chromatography     cellular fatty acids     dendrogram     typing    

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Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

Fanghe LI BM , Chunyan ZHANG MS , Jinghua LIU MS , Xiaoyan ZHANG MS , Bing YAN , Bo ZHANG MS , YongguoHUANG MS , Jingsong GONG BM , Yan CHEN BM ,

《医学前沿（英文）》 2009年 第3卷 第3期   页码 277-283 doi: 10.1007/s11684-009-0047-0

摘要： Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay (ELISA), a hybridoma cell line strain secreting anti-HBs monoclonal antibody (mAb) (defined G6 mAb) was obtained. The cells grew and secreted mAb stably. Antibody titers in the culture supernatant and ascites were 2.048×10 and 4.096×10, respectively. By applying the anti-HBs G6 mAb and horseradish peroxidase (HRP)-labeled goat anti-HBs antibody, we developed a sandwich ELISA (defined G6m ELISA) for detecting both wild-type and immune escape mutant HBsAgs (IEM HBsAg). The assay was performed to detect 17 species of genome recombinant expression HBsAg, including two wild-type species and 15 IEM HBsAg species, which varied in the “a” determinant, in a group of patients infected with hepatitis B virus (HBV). The patients previously had a lower ELISA detection signal [(absorbance of patients/absorbance of normal people (P/N): 1.0―4.5)]. The results demonstrated that the sensitivity of this assay to wild-type HBsAg was no less than 0.125g/L; 12 of 15 IEM HBsAg species (P/N≥2.5) were positive for G6 mAb. Of the positive IEM HBsAg species, two had a low absorbance value at 450nm (), one had an intermediate value and nine had a high value, which was 7.55%(mean), 59.4% and 92.1%―109.4% of the wild-type value, respectively. The two species with low value and the three negative species mutated at the bases 120―124 in the first loop of the HBV “a” determinant. Using the G6 ELISA and two commercial ELISA kits (A and B), 177 patients were tested. The G6 ELISA had a significantly higher detection rate than either commercial ELISAs (19.21% 14.89% and 6.21%, respectively; <0.01, <0.05, respectively).

关键词： hepatitis B virus     gene variation     hepatitis B surface antigen     immune escape     enzyme-linked immunosorbent assay    

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Study on factors affecting TB/HIV co-infection in four counties of China

Shi-Ming CHENG MD, Yu-Ji LAI MS, Er-Yong LIU MS, Lin ZHOU MD, Xue-Jing WANG MS, Qiu-Lan CHEN MS, Dong-MingLI MS, Ning WANG MD,

《医学前沿（英文）》 2010年 第4卷 第2期   页码 185-191 doi: 10.1007/s11684-010-0040-7

摘要： The factors affecting case detection of tuberculosis (TB)/human immunodeficiency virus (HIV) co-infection cases were explored in order to provide evidence-based formulation of a TB/HIV co-infection control strategy in China. Four counties with different modes of HIV transmission and varying cooperation mechanisms between TB and HIV institutions were selected. HIV test among TB patients and TB examination among people living with HIV/acquired immunodeficiency syndrome (AIDS) were conducted. Patients also completed questionnaires on potential factors affecting TB/HIV co-infection. The results showed that 19 (1.7%) were HIV-positive among 1089 cases of people living with active TB who received HIV testing. Conversely, there were 126 (10%) cases of active TB among 1255 cases of people living with HIV/AIDS. Among the newly discovered 145 cases of TB/HIV co-infection, AIDS institutions found 86.9% of these co-infections and 14.1% came from TB institutes. In different registration categories, there were 31 (21.4%) cases of smear positive TB, 104 (71.7%) cases of smear negative TB, and extrapulmonary cases accounted for 10 (6.9%). Gender, occupation, marital status, ethnic group, education, HIV transmission route, CD4 level, and type of TB susceptible symptoms had significant effects on whether HIV/AIDS patients were co-infected with TB disease (<0.01). Sex, age, ethnic group, occupation, and type of tuberculosis had no significant influence on whether TB patients had HIV infection. AIDS institutions could find more TB/HIV co-infection patients in comparison with TB institutions, particularly in patients with smear negative pulmonary tuberculosis and extrapulmonary tuberculosis. Therefore, a cooperation mechanism should be built up between TB and HIV control systems, and routine TB checks should be conducted for HIV/AIDS patients. In areas where sex or drug use is the major transmission mode of HIV infection, antiretroviral therapy should be expanded, treatment adherence should be improved and patients’ immunity level should be increased in order to decrease TB incidence. Cough and expectoration of more than two weeks, bloody sputum, and recurrent fevers could be revealing symptoms in TB screening of HIV/AIDS patients.

关键词： tuberculosis/acquired immunodeficiency syndrome     dual infection     prevention and control     case detection     impact factors    

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LC/MS/MS and radioisotope method combined for recognizing the affinity between catalpol and OCT2 transporter

Zhiyu ZHANG, Changxiao LIU, Duanyun SI, Rong LU, Xiulin YI

《化学科学与工程前沿（英文）》 2012年 第6卷 第4期   页码 436-442 doi: 10.1007/s11705-012-1222-4

摘要： More and more herbal medicines are found to be the substrates of drug transporters. In this paper, chromatography/tandem mass spectrometry (LC/MS/MS) combined with radioisotope method was used for the quantification of catalpol, a traditional Chinese medicine, to study the affinity relationship between herbal medicines and transporters. Catalpol uptake experiment was carried out by using several transporters (OAT1, OCT2, OAT3, OATP1B1 and OATP2B1). And samples were precipitated with methanol and quantified with LC/MS/MS. The results show that catalpol has a good affinity with OCT2-transfected S2 cells. After studying drug-drug interaction between catalpol and C-tetraethylammonium (TEA), we found that catalpol is able to facilitate TEA transport mediated by OCT2, suggesting that catalpol could probably be a new promoter of OCT2.

关键词： catalpol     LC/MS/MS     OCT2    

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HPLC determination of glyoxal in aldehyde solution with 3-methyl-2-benzothiazolinone hydrazone

Yamei ZHU, Xiaoli YAO, Shaohui CHEN, Qun CUI, Haiyan WANG

《化学科学与工程前沿（英文）》 2011年 第5卷 第1期   页码 117-121 doi: 10.1007/s11705-010-0535-4

摘要： Based on the absorption property of a diazine that can be formed by reaction of glyoxal and 3-methyl-2-benzothiazolinone hydrazone (MBTH) in the Ultraviolet-visible (UV-vis) spectral region, a HPLC method was developed for the determination of glyoxal in acetaldehyde solution. Glyoxal was derivatised from MBTH and the derivatives (diazine) were analyzed by HPLC for identification and quantification. The determination was performed on a ZORBAX Eclipse XDB-C18 column (4.6 × 250 mm, 5 mm) at 35°C with an injection volume of 10 mL, using a mixture of acetonitrile-water solvent (99∶5, v∶v) as a mobile phase with a flow rate of 0.8 mL·min . The proper derivative reaction conditions were the temperature of 70°C, MBTH to carbonyl molar ratio of 12, and reaction time of 110 min. The glyoxal diazine was a yellow dye with a maximum molar absorptivity at 401 nm and its retention time was 5.2 min under optimal HPLC conditions. The standard curve for glyoxal had a strong linear relationship with a regression coefficient ( = 0.999) in the range of 0.002–0.020 g·L . The analysis of glyoxal in an oxidising solution gave accurate results with a relative standard deviation (RSD) value of 0.55%. The average relative recovery was 102%. This efficient HPLC technique is also proposed for detecting other dicarbonyl compounds besides glyoxal.

关键词： HPLC     glyoxal     3-methyl-2-benzothiazolinone hydrazone     diazine     dicarbonyl compounds    

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A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host, Pomacea canaliculata

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

《医学前沿（英文）》 2010年 第4卷 第2期   页码 204-207 doi: 10.1007/s11684-010-0043-4

摘要： Pooling field specimens could reduce the number of assay and thus increase the efficiency in detecting and screening pathogen infections by polymerase chain reaction (PCR)-based assay. We investigated a pooling strategy in diagnosis of in Two settings of specimens were prepared, divided into portions and detected by multiplex PCR. Specimens A was 0.4490 g positive lung tissue of 28 larval nodes from four snails mixed with 1.310€g negative lung tissue from six snails and divided into 32 portions. Specimens B was 0.5448 g positive lung tissue with 26 larval nodes from two snails mixed with 1.092 g negative lung tissue from seven snails and divided into 48 portions. Repeated sampling was performed and sample size-accumulated positive rate curves were drawn. According to the sample size-accumulated positive rate curves, the appropriate sample size of the two specimens were 18 and 15, respectively, which is 0.36–0.58 to the total sample size. These test characteristics and the relevant factors to the sample size would need to be determined in much larger studies and more appropriately in field populations. The result indicates that the number of larval node is not the most important, nor the only factor to the sample size. It also implies the feasibility to detect in by pooling strategies.

关键词： Angiostrongylus cantonensis     Pomacea canaliculata     multiplex polymerase chain reaction     diagnosis     pooling    

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印度洋8.7级与8.5级地震的物理前兆

孙威,孙晓明

《中国工程科学》 2008年 第10卷 第2期   页码 14-25

摘要：

采用自主研发的地震应力仪、谐振仪 、倾斜仪和大地电位仪记录到印度洋8.7级大震的地震前兆——孕震物理模型和临震信号， 印度洋8.7级地震前后和8.5级地震前后的地壳受力情况有很大差异，表明 印度洋8.7级地震与8.5级地震的成因有着本质的区别，分析了海啸形成的原因，还发现了在 8.7级地震的孕育过程中有地下物质迁移迹象。

关键词： 印度洋8.7级与8.5级地震     临震信号     顺势脉冲     逆势脉冲    

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The inactivation of bacteriophages MS2 and PhiX174 by nanoscale zero-valent iron: Resistance difference

《环境科学与工程前沿（英文）》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1529-4

摘要：

• The resistance of phage PhiX174 to nZVI was much stronger than that of MS2.

关键词： Nanoscale zero-valent iron (nZVI)     MS2     PhiΧ174     Resistance     Inactivation     Pathogenic microorganisms    

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Mature cystic teratoma of the parotid gland: a case report and review of the literature

Lenan SHAO DMD, MD, Hanxiong GUAN MD, Jie WAN MS,

《医学前沿（英文）》 2009年 第3卷 第4期   页码 503-506 doi: 10.1007/s11684-009-0076-8

摘要： We report one case of mature cystic teratoma that presented as a tender, well-defined round mass in the right parotid gland region. Teratomas are common neoplasms but are very rarely found in the parotid gland region, so diagnosis and treatment when such occurs is a challenge. Magnetic resonance imaging scanning is useful in determining the nature of teratomas, and only surgery and pathology can provide a final diagnosis. The treatment strategy is to preserve both the facial nerve and the surrounding parotid gland tissue, while completely extirpating the teratoma.

关键词： teratoma     parotid gland    

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Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on Persistent Organic Pollutants (2008–2019)

《环境科学与工程前沿（英文）》 2022年 第16卷 第10期 doi: 10.1007/s11783-022-1541-8

摘要：

● Perfluorooctanesulfonic acid and perfluorooctanoic acid highest in human milk.

关键词： Human biomonitoring     Human breast milk     LC-MS/MS analysis     Lifestyle parameters    

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and bacteriophage MS2 disinfection by UV, ozone and the combined UV and ozone processes

Jingyun FANG,Huiling LIU,Chii SHANG,Minzhen ZENG,Mengling NI,Wei LIU

《环境科学与工程前沿（英文）》 2014年 第8卷 第4期   页码 547-552 doi: 10.1007/s11783-013-0620-2

摘要： The combination of low-dose ozone with ultraviolet (UV) irradiation should be an option to give benefit to disinfection and reduce drawbacks of UV and ozone disinfection. However, less is known about the disinfection performance of UV and ozone (UV/ozone) coexposure and sequential UV-followed-by-ozone (UV-ozone) and ozone-followed-by-UV (ozone-UV) exposures. In this study, inactivation of and bacteriophage MS2 by UV, ozone, UV/ozone coexposure, and sequential UV-ozone and ozone-UV exposures was investigated and compared. Synergistic effects of 0.5–0.9 log kill on inactivation, including increases in the rate and efficiency, were observed after the UV/ozone coexposure at ozone concentrations as low as 0.05 mg·L in ultrapure water. The coexposure with 0.02-mg·L ozone did not enhance the inactivation but repressed photoreactivation. Little enhancement on inactivation was found after the sequential UV-ozone or ozone-UV exposures. The synergistic effect on MS2 inactivation was less significant after the UV/ozone coexposure, and more significant after the sequential ozone-UV and UV-ozone exposures, which was 0.2 log kill for the former and 0.8 log kill for the latter two processes, at ozone dose of 0.1 mg·L and UV dose of 8.55 mJ·cm in ultrapure water. The synergistic effects on disinfection were also observed in tap water. These results show that the combination of UV and low-dose ozone is a promising technology for securing microbiological quality of water.

关键词： bacteria inactivation     photoreactivation     water disinfection     UV     ozone    

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标题 作者 时间 类型 操作

Analysis of two new degradation products of arsenic triglutathione in aqueous solution

Feng ZHAO, Yuchen CHEN, Bin QIAO, Jing WANG, Ping NA

期刊论文

从天然产物创制新药的发展趋势

于德泉

期刊论文

Photocatalytic degradation of methyl orange using ZnO/TiO composites

Ming GE , Changsheng GUO , Xingwang ZHU , Lili MA , Wei HU , Yuqiu WANG , Zhenan HAN ,

期刊论文

Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis

Yansong HOU, Wei LIANG, Liping ZHANG, Shuiping CHENG, Feng HE, Zhenbin WU

期刊论文

Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAOMS,

期刊论文

Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

Fanghe LI BM , Chunyan ZHANG MS , Jinghua LIU MS , Xiaoyan ZHANG MS , Bing YAN , Bo ZHANG MS , YongguoHUANG MS , Jingsong GONG BM , Yan CHEN BM ,

期刊论文

Study on factors affecting TB/HIV co-infection in four counties of China

Shi-Ming CHENG MD, Yu-Ji LAI MS, Er-Yong LIU MS, Lin ZHOU MD, Xue-Jing WANG MS, Qiu-Lan CHEN MS, Dong-MingLI MS, Ning WANG MD,

期刊论文

LC/MS/MS and radioisotope method combined for recognizing the affinity between catalpol and OCT2 transporter

Zhiyu ZHANG, Changxiao LIU, Duanyun SI, Rong LU, Xiulin YI

期刊论文

HPLC determination of glyoxal in aldehyde solution with 3-methyl-2-benzothiazolinone hydrazone

Yamei ZHU, Xiaoli YAO, Shaohui CHEN, Qun CUI, Haiyan WANG

期刊论文

A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host, Pomacea canaliculata

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

期刊论文

印度洋8.7级与8.5级地震的物理前兆

孙威,孙晓明

期刊论文

The inactivation of bacteriophages MS2 and PhiX174 by nanoscale zero-valent iron: Resistance difference

期刊论文

Mature cystic teratoma of the parotid gland: a case report and review of the literature

Lenan SHAO DMD, MD, Hanxiong GUAN MD, Jie WAN MS,

期刊论文

Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on Persistent Organic Pollutants (2008–2019)

期刊论文

and bacteriophage MS2 disinfection by UV, ozone and the combined UV and ozone processes

Jingyun FANG,Huiling LIU,Chii SHANG,Minzhen ZENG,Mengling NI,Wei LIU

期刊论文