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Effect of IL-10 on formation of foam cell induced by ox-LDL

WANG Fei, DAI Yalei, XU Ting, XU Bo, WANG Kaifeng

《医学前沿(英文)》 2008年 第2卷 第3期   页码 298-302 doi: 10.1007/s11684-008-0057-3

摘要: Atherosclerosis is a chronic disease that causes various cardiovascular complications. It has been realized that cellular and humoral immunity plays crucial roles in atherogenic lesion formation. In this study the effects of lipopolysaccharide (LPS) and interleukin-10 (IL-10) on the formation of foam cells during the early stages of atherosclerosis have been investigated. Macrophage was induced by phorbol myristate acetate (PMA) treatment on THP-1 cells. The cells were further stimulated by ox-LDL, ox-LDL plus LPS, ox-LDL plus IL-10 and LPS. By using an oil red O staining technique, the formation of foam cells was evaluated by lipid granules formation in the cells. The ratio of foam cell formation was increased from (9.77 ± 1.70)% to (16.27 ± 2.27)% after 24 h stimulation with ox-LDL, and the increase was observed with incubating time. The foam cells were significantly increased in the presence of LPS in a dose-dependent manner. The maximum increase of about 40% was observed. However, the significant elevation by LPS was abrogated when IL-10 was added. These results indicated that IL-10 can effectively prevent the formation of foam cells induced by ox-LDL with or without LPS. This study demonstrates that ox-LDL can cause foam cell formation from macrophages . LPS can significantly accelerate this event. IL-10, an anti-inflammatory cytokine, can inhibit the effect of ox-LDL and LPS. These results indicate that inflammatory effects in blood vessels can speed up foam cell formation. The inhibitive effect of IL-10 is an important factor for delaying atherosclerosis processes.

关键词: inflammatory     presence     interleukin-10     dose-dependent     ox-LDL    

Low-dose CT for lung cancer screening: opportunities and challenges

null

《医学前沿(英文)》 2018年 第12卷 第1期   页码 116-121 doi: 10.1007/s11684-017-0600-1

摘要:

Lung cancer is among the most frequently diagnosed cancers worldwide and the leading cause of cancer death in both males and females. Screening for lung cancer coupled with earlier intervention has long been studied as an approach to mortality reduction. However, minimal progress was achieved until recently, when low-dose spiral computed tomography (LDCT) screening demonstrated a 20% reduction in mortality from lung cancer in a randomized controlled trial (RCT), the National Lung Screening Trial, from the United States. On the basis of this finding, LDCT has been recommended for lung cancer screening in high-risk populations by several clinical guidelines. However, results from the following independent RCTs in Europe failed to show consistent conclusions. In addition, intractable problems gradually emerged with the progress of LDCT screening. This paper summarizes and discusses the main observations and challenges of LDCT screening for lung cancer. Before spreading implementation of LDCT screening, challenges, including high false-positive rates, overdiagnosis, enormous costs, and radiation risk, must be addressed. Complementary biomarkers and technical improvement are expected in the field of lung cancer screening in the near future.

关键词: lung cancer     low-dose computerized tomography     early detection     opportunities     challenges    

Estimation of the minimum effective dose of tramadol for postoperative analgesia in infants using the

null

《医学前沿(英文)》 2012年 第6卷 第3期   页码 288-295 doi: 10.1007/s11684-012-0208-4

摘要:

Tramadol is a potent analgesic. However, the analgesia efficacy of tramadol, particularly its minimum effective dose (MED), is not clear. The aim of this study is to find MED of tramadol for postoperative analgesia in infants. The continual reassessment method (CRM) was performed to find MED. Infants undergoing surgeries were included in the 3 phases of this series. In each phase, 24 participants were allocated a different tramadol dose. Pain intensity was measured by face, legs, activity, cry, consolability (FLACC) measurement at 3-hour intervals. Tramadol was considered ineffective if the FLACC score was higher than 4 in 10 at anytime. In phase 1, seven dose levels were used within the range 0.1–0.4 mg?kg-1·h-1. Phase 1 was insufficient to identify the MED, and we increased the dose to 0.4–0.8 mg?kg-1·h-1 in phase 2. Phase 2 was insufficient to identify the MED. In phase 3, local anesthetic wound infiltration was introduced, and the tramadol dose levels tested were the same as in phase 1. The successful analgesia probability of tramadol 0.4 mg?kg-1?h-1 was 82.1% (95% CI, 0.742–0.925) in phase 1. In phase 2, it was 84.7% (95% CI, 0.789–0.991) with the dose 0.8 mg?kg-1?h-1. Phase 1 and phase 2 were insufficient to identify the MED. In phase 3, the successful analgesia probability for dose 0.35 mg?kg-1?h-1was 96.7% (95% CI, 0.853–0.997).We have demonstrated that tramadol provides insufficient analgesia for surgeries considered to cause moderate-to-severe postoperative pain in infants if used as the sole analgesic, and that local anesthetic wound infiltration enhances the efficacy of tramadol.

关键词: tramadol     minimum effective dose     postoperative analgesia     infants     continual reassessment method    

Comparison of exogenous degrader-enhanced bioremediation with low-dose persulfate oxidation for polycyclic

《环境科学与工程前沿(英文)》 2023年 第17卷 第11期 doi: 10.1007/s11783-023-1733-x

摘要: Polycyclic aromatic hydrocarbon (PAH)-contaminated soils are usually complex and characterized by a lack of nutrition and soil salinization, resulting in difficulties in soil remediation. In this study, bioaugmentation with a PAH-degrading Bacillus PheN7 (BA) and low-dose persulfate oxidation (PS), along with natural biodegradation, were utilized to remediate alkaline PAH-contaminated soil. The soil used in the study had a pH of 9.35, and the total PAH content was 568.8 ± 31.0 mg/kg dry soil. After 42 d of remediation, the degradation efficiency of PAHs was 96.72% and 93.88% using persulfate oxidation and bioaugmentation, respectively, whereas 38.66% of PAHs were degraded in natural attenuation (NA). Bacillus was the dominant genera throughout the process of bioremediation with the relative abundance of 79.3% on day 42 in the BA system, whereas, Alcanivorax was enriched and became the dominant genera in PS systems. In the meantime, PAH degradation genes were detected with remarkably higher level in the BA system than in PS system during the remediation. In addition to the degradation of contaminants, persulfate oxidation promotes microbial bioremediation efficiency mainly by lowering the pH to neutral and increasing the active phosphorus content in the soil. Microbial species and ecological niches were less reduced in the PS system than in the BA system. Collectively, persulfate oxidation had a better impact on the soil microbiome and is more suitable for long-term soil health than bioaugmentation through PheN7 addition.

关键词: Bioaugmentation     Low-dose persulfate oxidation     Polycyclic aromatic hydrocarbon     Remediation    

Early assessment of the safety and immunogenicity of a third dose (booster) of COVID-19 immunization

《医学前沿(英文)》 2022年 第16卷 第1期   页码 93-101 doi: 10.1007/s11684-021-0914-x

摘要: Inducing durable and effective immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via vaccination is essential to combat the current pandemic of coronavirus disease 2019 (COVID-19). It has been noticed that the strength of anti-COVID-19 vaccination-induced immunity fades over time, which calls for an additional vaccination regime, as known as booster immunization, to restore immunity among previously vaccinated populations. Here we report a pilot open-label trial of a third dose of BBIBP-CorV, an inactivated SARS-CoV-2 vaccine (Vero cell), on 136 participants aged between 18 to 63 years. Safety and immunogenicity in terms of neutralizing antibody titers and cytokine/chemokine responses were analyzed as the main endpoint until day 28. While systemic reactogenicity was either absent or mild, SARS-CoV-2-specific neutralizing antibody titers rapidly arose in all participants within 4 weeks, surpassing the peak antibody titers elicited by the initial two-dose immunization regime. Broad increases of cellular immunity-associated cytokines and chemokines were also detected in the majority of participants after the third vaccination. Furthermore, in an exploratory study, a newly developed recombinant protein vaccine, NVSI-06-08 (CHO Cells), was found to be safe and even more effective than BBIBP-CorV in eliciting humoral immune responses in BBIBP-CorV-primed individuals. Together, these results indicate that a third immunization schedule with either homologous or heterologous vaccine showed favorable safety profiles and restored potent SARS-CoV-2-specific immunity, providing support for further trials of booster vaccination in larger populations.

关键词: COVID-19     SARS-CoV-2     vaccine     immunization     booster immunization    

Data-driven approach to solve vertical drain under time-dependent loading

《结构与土木工程前沿(英文)》 2021年 第15卷 第3期   页码 696-711 doi: 10.1007/s11709-021-0727-7

摘要: Currently, the vertical drain consolidation problem is solved by numerous analytical solutions, such as time-dependent solutions and linear or parabolic radial drainage in the smear zone, and no artificial intelligence (AI) approach has been applied. Thus, in this study, a new hybrid model based on deep neural networks (DNNs), particle swarm optimization (PSO), and genetic algorithms (GAs) is proposed to solve this problem. The DNN can effectively simulate any sophisticated equation, and the PSO and GA can optimize the selected DNN and improve the performance of the prediction model. In the present study, analytical solutions to vertical drains in the literature are incorporated into the DNN–PSO and DNN–GA prediction models with three different radial drainage patterns in the smear zone under time-dependent loading. The verification performed with analytical solutions and measurements from three full-scale embankment tests revealed promising applications of the proposed approach.

关键词: vertical drain     artificial neural network     time-dependent loading     deep learning network     genetic algorithm     particle swarm optimization    

An investigation on patient dose in screen-film diagnostic radiology in Lhasa City, Xizang Autonomous

null

《医学前沿(英文)》 2013年 第7卷 第4期   页码 506-509 doi: 10.1007/s11684-013-0294-y

摘要:

This study aimed to investigate patient dose in diagnostic screen-film radiographic examinations in the city of Lhasa, China. Seven out of the twenty-six hospitals registered with the Lhasa Health Bureau were included in the investigation. The entrance surface air Kerma (ESAK) of seven conventional screen-film radiology X-ray equipment in these hospitals was measured with a QA dosimeter in September 2012. The X-ray examinations were divided into three categories: PA (posterior-anterior) chest, upper/lower limb, and AP (anterior-posterior) lumbar spine. For each category, ESAKs were calculated and analyzed. The mean ESAK was 0.6 mGy for PA chest, 0.3 mGy for upper/lower limb, and 1.8 mGy for AP lumbar spine. In addition, the mean ESAK value recorded for PA chest X-ray examinations exceeded the corresponding value recommended by the International Atomic Energy Agency (0.4 mGy).

关键词: radiation dosage     radiology     radiation protection     radiography/statistics & numerical data    

Chloride binding and time-dependent surface chloride content models for fly ash concrete

S. MUTHULINGAM,B. N. RAO

《结构与土木工程前沿(英文)》 2016年 第10卷 第1期   页码 112-120 doi: 10.1007/s11709-015-0322-x

摘要: Corrosion of embedded rebars is a classical deterioration mechanism of reinforced concrete structures exposed to chloride environments. Such environments can be attributed to the presence of seawater, deicing or sea-salts, which have high concentrations of chloride ion. Chloride ingress into concrete, essential for inducing rebar corrosion, is a complex interaction between many physical and chemical processes. The current study proposes two chloride ingress parameter models for fly ash concrete, namely: 1) surface chloride content under tidal exposure condition; and 2) chloride binding. First, inconsistencies in surface chloride content and chloride binding models reported in literature, due to them not being in line with past research studies, are pointed out. Secondly, to avoid such inconsistencies, surface chloride content and chloride binding models for fly ash concrete are proposed based upon the experimental work done by other researchers. It is observed that, proposed models are simple, consistent and in line with past research studies reported in literature.

关键词: binding isotherms     chloride ingress     concrete     fly ash     surface chloride content    

GDF15 negatively regulates chemosensitivity via TGFBR2-AKT pathway-dependent metabolism in esophageal

《医学前沿(英文)》 2023年 第17卷 第1期   页码 119-131 doi: 10.1007/s11684-022-0949-7

摘要: Treating patients with esophageal squamous cell carcinoma (ESCC) is challenging due to the high chemoresistance. Growth differentiation factor 15 (GDF15) is crucial in the development of various types of tumors and negatively related to the prognosis of ESCC patients according to our previous research. In this study, the link between GDF15 and chemotherapy resistance in ESCC was further explored. The relationship between GDF15 and the chemotherapy response was investigated through in vitro and in vivo studies. ESCC patients with high levels of GDF15 expression showed an inferior chemotherapeutic response. GDF15 improved the tolerance of ESCC cell lines to low-dose cisplatin by regulating AKT phosphorylation via TGFBR2. Through an in vivo study, we further validated that the anti-GDF15 antibody improved the tumor inhibition effect of cisplatin. Metabolomics showed that GDF15 could alter cellular metabolism and enhance the expression of UGT1A. AKT and TGFBR2 inhibition resulted in the reversal of the GDF15-induced expression of UGT1A, indicating that TGFBR2-AKT pathway-dependent metabolic pathways were involved in the resistance of ESCC cells to cisplatin. The present investigation suggests that a high level of GDF15 expression leads to ESCC chemoresistance and that GDF15 can be targeted during chemotherapy, resulting in beneficial therapeutic outcomes.

关键词: GDF15     esophageal squamous cell carcinoma     chemoresistance     cellular metabolism     TGFBR2     AKT    

Effect of chemical dose on phosphorus removal and membrane fouling control in a UCT-MBR

Guangrong Sun, Chuanyi Zhang, Wei Li, Limei Yuan, Shilong He, Liping Wang

《环境科学与工程前沿(英文)》 2019年 第13卷 第1期 doi: 10.1007/s11783-019-1085-8

摘要: To enhance phosphorus removal and make the effluent meet the strict discharge level of total phosphorus (TP, 0.5 mg/L), flocculant dosing is frequently applied. In this study, the performance of aluminum sulfate dosing in a University of Cape Town Membrane Bioreactor (UCT-MBR) was investigated, in terms of the nutrients removal performance, sludge characteristics and membrane fouling. The results indicated that the addition of aluminum sulfate into the aerobic reactor continuously had significantly enhanced phosphorus removal. Moreover, COD, NH -N and TN removal were not affected and effluent all met the first level A criteria of GB18918-2002. In addition, the addition of aluminum sulfate had improved the sludge activity slightly and reduced trans-membrane pressure (TMP) increase rate from 1.13 KPa/d to 0.57 KPa/d effectively. The membrane fouling was alleviated attributed to the increased average particle sizes and the decreased accumulation of the small sludge particles on membrane surface. Furthermore, the decline of extracellular polymeric substance (EPS) concentration in mixed sludge liquid decreased its accumulation on membrane surface, resulting in the mitigation of membrane fouling directly.

关键词: University of Cape Town Bioreactor (UCT-MBR)     enhanced nutrients removal     aluminum sulfate     sludge activity     membrane fouling    

radiohormonal therapy for oligo-metastatic prostate cancer: safety and efficacy outcomes from an open-label, dose-escalation

《医学前沿(英文)》 2023年 第17卷 第2期   页码 231-239 doi: 10.1007/s11684-022-0939-9

摘要: To evaluate the safety and efficacy of neoadjuvant radiohormonal therapy for oligometastatic prostate cancer (OMPC), we conducted a 3 + 3 dose escalation, prospective, phase I/II, single-arm clinical trial (CHiCTR1900025743), in which long-term neoadjuvant androgen deprivation was adopted 1 month before radiotherapy, comprising intensity modulated radiotherapy to the pelvis, and stereotactic body radiation therapy to all extra-pelvic bone metastases for 4‒7 weeks, at 39.6, 45, 50.4, and 54 Gy. Robotic-assisted radical prostatectomy was performed after 5‒14 weeks. The primary outcome was treatment-related toxicities and adverse events; secondary outcomes were radiological treatment response, positive surgical margin (pSM), postoperative prostate-specific antigen (PSA), pathological down-grading and tumor regression grade, and survival parameters. Twelve patients were recruited from March 2019 to February 2020, aging 66.2 years in average (range, 52‒80). Median baseline PSA was 62.0 ng/mL. All underwent RARP successfully without open conversions. Ten patients recorded pathological tumor down-staging (83.3%), and 5 (41.7%) with cN1 recorded negative regional lymph nodes on final pathology. 66.7% (8/12) recorded tumor regression grading (TRG) –I and 25% (3/12) recorded TRG-II. Median follow-up was 16.5 months. Mean radiological progression-free survival (RPFS) was 21.3 months, with 2-year RPFS of 83.3%. In all, neoadjuvant radiohormonal therapy is well tolerated for oligometastatic prostate cancer.

关键词: neoadjuvant     radiotherapy     oligometastatic     prostate cancer     radical prostatectomy    

Analysis of dispatching rules in a stochastic dynamic job shop manufacturing system with sequence-dependent

Pankaj SHARMA,Ajai JAIN

《机械工程前沿(英文)》 2014年 第9卷 第4期   页码 380-389 doi: 10.1007/s11465-014-0315-9

摘要:

Stochastic dynamic job shop scheduling problem with consideration of sequence-dependent setup times are among the most difficult classes of scheduling problems. This paper assesses the performance of nine dispatching rules in such shop from makespan, mean flow time, maximum flow time, mean tardiness, maximum tardiness, number of tardy jobs, total setups and mean setup time performance measures viewpoint. A discrete event simulation model of a stochastic dynamic job shop manufacturing system is developed for investigation purpose. Nine dispatching rules identified from literature are incorporated in the simulation model. The simulation experiments are conducted under due date tightness factor of 3, shop utilization percentage of 90 % and setup times less than processing times. Results indicate that shortest setup time (SIMSET) rule provides the best performance for mean flow time and number of tardy jobs measures. The job with similar setup and modified earliest due date (JMEDD) rule provides the best performance for makespan, maximum flow time, mean tardiness, maximum tardiness, total setups and mean setup time measures.

关键词: scheduling     stochastic dynamic job shop     sequence-dependent setup times     dispatching rule     simulation    

Cancer and the environment: Filling knowledge gaps together

Linda S. BIRNBAUM PhD, DABT, ATS,

《医学前沿(英文)》 2010年 第4卷 第2期   页码 131-133 doi: 10.1007/s11684-010-0044-3

摘要: When considering disease etiology, we need to view the role of the environment along the continuum from health to disease for individuals because we know that there are complex interactions between genes, their molecular expression, and environmental factors over a person’s lifetime. We clearly have huge gaps in our knowledge along this continuum, and these gaps are natural opportunities for research. There are many factors to consider as we assess the relationship between environmental exposures over a lifetime. One factor is persistence of chemicals that “live” beyond their initial intended use. Another factor is the fact that “inert ingredients” are not really inert. “Low dose” is also another consideration. We need to understand the biological effect of low dose exposure and we should clearly define what they mean by low dose research. For example, are we measuring the administered dose, or the internal/circulating dose? There are new types of toxicity as well. For example, endocrine disruptors and engineered nanomaterials are significant classes of materials deserving significant research. Timing of exposure is a significant feature in characterizing toxicity. Environmental exposures can have a greater impact in early or later life-stages. Finally, mixtures of chemicals are an important consideration. Humans are always exposed to multiple chemicals. In short, we must remember that there is no such thing as “not exposed.” Everyone has been exposed to many and various environmental agents.

关键词: environment     genes     environmental exposure     environmental agent     persistence     chemicals     inert ingredients     low dose    

Venetoclax and low-dose cytarabine induced complete remission in a patient with high-risk acute myeloid

Bingshan Liu, Roshni Narurkar, Madhura Hanmantgad, Wahib Zafar, Yongping Song, Delong Liu

《医学前沿(英文)》 2018年 第12卷 第5期   页码 593-599 doi: 10.1007/s11684-018-0635-y

摘要:

Conventional combination therapies have not resulted in considerable progress in the treatment of acute myeloid leukemia (AML). Elderly patients with AML and poor risk factors have grave prognosis. Midostaurin has been recently approved for the treatment of FLT-3-mutated AML. Venetoclax, a BCL-2 inhibitor, has been approved for the treatment of relapsed and/or refractory chronic lymphoid leukemia. Clinical trials on applying venetoclax in combination with cytarabine and other agents to treat various hematological malignancies are currently underway. Here, we present a case of a male patient with poor performance status and who developed AML following allogeneic hematopoietic stem cell transplant for high-risk myelodysplasia. The patient with high risk AML achieved complete response to the combined treatment regimen of low-dose cytarabine and venetoclax. Furthermore, we reviewed current clinical trials on the use of venetoclax for hematological malignancies.

关键词: venetoclax     cytarabine     AML     leukemia    

A rate-dependent Prandtl-Ishlinskii model for piezoelectric actuators using the dynamic envelope function

Meiju YANG,Chunxia LI,Guoying GU,Limin ZHU

《机械工程前沿(英文)》 2015年 第10卷 第1期   页码 37-42 doi: 10.1007/s11465-015-0326-1

摘要:

In this paper, a novel rate-dependent Prandtl-Ishlinskii (P-I) model is proposed to characterize the rate-dependent hysteresis nonlinearity of piezoelectric actuators. The new model is based on a modified rate-dependent play operator, in which a dynamic envelope function is introduced to replace the input function of the classical play operator. Moreover, a dynamic density function is utilized in the proposed P-I model. The parameters of the proposed model are identified by a modified particle swarm optimization algorithm. Finally, experiments are conducted on a piezo-actuated nanopositioning stage to validate the proposed P-I model under the sinusoidal inputs. The experimental results show that the developed rate-dependent P-I model precisely characterize the rate-dependent hysteresis loops up to 1000 Hz.

关键词: piezoelectric actuators     hysteresis     Prandtl-Ishlinskii     rate-dependent    

标题 作者 时间 类型 操作

Effect of IL-10 on formation of foam cell induced by ox-LDL

WANG Fei, DAI Yalei, XU Ting, XU Bo, WANG Kaifeng

期刊论文

Low-dose CT for lung cancer screening: opportunities and challenges

null

期刊论文

Estimation of the minimum effective dose of tramadol for postoperative analgesia in infants using the

null

期刊论文

Comparison of exogenous degrader-enhanced bioremediation with low-dose persulfate oxidation for polycyclic

期刊论文

Early assessment of the safety and immunogenicity of a third dose (booster) of COVID-19 immunization

期刊论文

Data-driven approach to solve vertical drain under time-dependent loading

期刊论文

An investigation on patient dose in screen-film diagnostic radiology in Lhasa City, Xizang Autonomous

null

期刊论文

Chloride binding and time-dependent surface chloride content models for fly ash concrete

S. MUTHULINGAM,B. N. RAO

期刊论文

GDF15 negatively regulates chemosensitivity via TGFBR2-AKT pathway-dependent metabolism in esophageal

期刊论文

Effect of chemical dose on phosphorus removal and membrane fouling control in a UCT-MBR

Guangrong Sun, Chuanyi Zhang, Wei Li, Limei Yuan, Shilong He, Liping Wang

期刊论文

radiohormonal therapy for oligo-metastatic prostate cancer: safety and efficacy outcomes from an open-label, dose-escalation

期刊论文

Analysis of dispatching rules in a stochastic dynamic job shop manufacturing system with sequence-dependent

Pankaj SHARMA,Ajai JAIN

期刊论文

Cancer and the environment: Filling knowledge gaps together

Linda S. BIRNBAUM PhD, DABT, ATS,

期刊论文

Venetoclax and low-dose cytarabine induced complete remission in a patient with high-risk acute myeloid

Bingshan Liu, Roshni Narurkar, Madhura Hanmantgad, Wahib Zafar, Yongping Song, Delong Liu

期刊论文

A rate-dependent Prandtl-Ishlinskii model for piezoelectric actuators using the dynamic envelope function

Meiju YANG,Chunxia LI,Guoying GU,Limin ZHU

期刊论文