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2009, Volume 3, Issue 3

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Frontiers of Medicine >> 2009, Volume 3, Issue 3 doi: 10.1007/s11684-009-0057-y

Design of tandem genes cluster for isoflavone engineering

China-UK HUST-RRes Genetics Engineering and Genomics Joint Laboratory, Genetic Engineering International Cooperation Base of the Ministry of Science and Technology of China, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China;

Available online: 2009-09-05
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Abstract

In this paper, a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported. A tandem genes cluster () of secondary metabolites of plant isoflavones was constructed by using the chalcone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS) (GenBank accession numbers EU526827, EU526829, EU526830) in only one recombination with the pET22b vector. The resulting expression vector pET- was heterogeneously expressed and co-incubated with barrenwort extractions, and the genistein-like component was detected.

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