Frontiers of Medicine
>> 2009,
Volume 3,
Issue 3
doi:
10.1007/s11684-009-0064-z
Effect of decoction on CD14 expression in lipopolysaccharide signal transduction
pathway of alcohol-induced liver disease in rats
1.Department of Traditional
Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong
University of Science and Technology, Wuhan 430022, China; 2.Department of Gastroenterology,
Union Hospital, Tongji Medical College, Huazhong University of Science
and Technology, Wuhan 430022, China;
Available online: 2009-09-05
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Abstract
This paper aims to investigate the effects of (枳黄) decoction on CD14 expression in the lipopolysaccharide signal transduction pathway of alcohol-induced liver disease in rats. Seventy-five Wistar rats were randomly divided into three groups. Ethanol (56%, weight/volumn) was intragastrically administrated to 50 rats (14mL/kg body weight per day) for 10 days to establish a model of alcohol-induced liver disease, and 25 of these 50 rats were treated with decoction simultaneously. Liver injury was evaluated by biochemical examination. The plasma content of endotoxin was assayed by biochemistry. The expression of CD14 mRNA and protein in rat liver was measured by reverse transcriptional polymerase chain reaction and immunohistochemistry, respectively. decoction pretreatment significantly protected against acute alcohol-induced liver injury, which was evidenced by the decrease of elevated serum alanine aminotransferase and aspartate aminotransferase. In addition, the level of plasma endotoxin and up-regulation of CD14 was also suppressed by decoction in alcohol-intoxicated rats. decoction can significantly reduce CD14 expression in the lipopolysaccharide signal transduction pathway, which is one of the most important mechanisms of decoction to treat hepatic injury induced by alcohol in rats.
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