2015, Volume 9, Issue 1
Frontiers of Medicine >> 2015, Volume 9, Issue 1 doi: 10.1007/s11684-014-0337-z
Three-dimensional reconstruction of light microscopy image sections: present and future
Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns, and Combined Injury, Chongqing Key Laboratory for Diseases Proteomics, the Third Military Medical University, Chongqing 400038, China
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Abstract
Three-dimensional (3D) image reconstruction technologies can reveal previously hidden microstructures in human tissue. However, the lack of ideal, non-destructive cross-sectional imaging techniques is still a problem. Despite some drawbacks, histological sectioning remains one of the most powerful methods for accurate high-resolution representation of tissue structures. Computer technologies can produce 3D representations of interesting human tissue and organs that have been serial-sectioned, dyed or stained, imaged, and segmented for 3D visualization. 3D reconstruction also has great potential in the fields of tissue engineering and 3D printing. This article outlines the most common methods for 3D tissue section reconstruction. We describe the most important academic concepts in this field, and provide critical explanations and comparisons. We also note key steps in the reconstruction procedures, and highlight recent progress in the development of new reconstruction methods.
Keywords
microtomy ; 3D imaging ; computer-assisted image processing ; 3D printing ; tissue scaffold
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