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Structural prediction of porcine sialoadhesin V-set Ig-like domain sheds some light on its role in porcine reproductive and respiratory syndrome virus (PRRSV) infection

Jie HOU,Rui LI,Hongfang MA,Songlin QIAO,Gaiping ZHANG

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1,   Pages 65-71 doi: 10.15302/J-FASE-2016086

Abstract: In this study, we successfully cloned, expressed and purified the pSn V-set Ig-like domain in S2 cells

Keywords： PRRSV     porcine sialoadhesin     V-set Ig-like domain     <i>Drosophilai> S2 cell     crystallization    

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<i>Ni>-Positive ion activated rapid addition and mitochondrial targeting ratiometric fluorescent probesfor <i>in vivoi> cell H2S imaging

Yan Shi, Fangjun Huo, Yongkang Yue, Caixia Yin

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 1,   Pages 64-71 doi: 10.1007/s11705-021-2048-8

Abstract: of H S detection.As such, the lower detection limit can reach 55.7 nmol/L for H S.In addition, cell imaging shows that this probe can be used for the mitochondrial targeted detectionof endogenous and exogenous H S.Finally, this probe application was verified by imaging H S in nude mice.

Keywords： heterocyclic compound     hydrogen sulfide     ratiometric     mitochondrial targeted    

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NO<i>xi> and H2S formation in the reductive zone of air-staged combustion of

Jinzhi CAI, Dan LI, Denggao CHEN, Zhenshan LI

Frontiers in Energy 2021, Volume 15, Issue 1,   Pages 4-13 doi: 10.1007/s11708-020-0804-y

Abstract: China to control NO emission; thus, it is necessary to understand the formation mechanism of NO and H SThis paper focused on the investigation of reductive gases in the formation of NO and H S in the reductiveinto GE was found to increase not only the coal conversion but also the concentrations of CO and H SBoth the CO and H S concentrations inblended coal combustion increase with an increase in the combustionBased on accumulated experimental data, one interesting finding was that NO and H S from blended coal

Keywords： blended coal combustion     NO<i>x i>formation     H2S formation     air staged combustion    

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Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 4,   Pages 425-436 doi: 10.1007/s11705-022-2229-0

Abstract: Here, <i>αi>-acetolactate synthase and <i>αi>-acetolactate decarboxylase from <i>Bacillus subtilisi> 168 were co-expressed in <i>H. bluephagenesisi> to produce acetoin from pyruvate.Moreover, the reusability of the cell catalysis was also tested, and the result illustrated that thewhole-cell catalysis obtained 433.3, 440.2, 379.0, 442.8 and 339.4 mmol·L–1 (38.2, 38.8, 33.4This work therefore provided an efficient <i>H. bluephagenesisi> whole-cell catalysis with a broad

Keywords： acetoin     pyruvate     <i>αi>-acetolactate synthetase     <i>αi>-acetolactate decarboxylase     <i>Halomonasbluephagenesisi>     whole-cell biocatalysis    

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Aerodynamic design by jointly applying S2 flow surface calculation and modern optimization methods on

ZHAO Honglei, WANG Songtao, HAN Wanjin, FENG Guotai

Frontiers in Energy 2008, Volume 2, Issue 1,   Pages 93-98 doi: 10.1007/s11708-008-0007-4

Abstract: A three-stage axial turbine was redesigned by jointly applying S2 flow surface direct problem calculation
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<i>FESEi>’s Best Papers of 2017

Frontiers of Environmental Science & Engineering 2018, Volume 12, Issue 3, doi: 10.1007/s11783-018-1051-x

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Accounting for the uncertainties in the estimation of average shear wave velocity using – correlations

Frontiers of Structural and Civil Engineering 2021, Volume 15, Issue 5,   Pages 1199-1208 doi: 10.1007/s11709-021-0749-1

Abstract: Shear wave velocity ( <i>Vi> S) is a key parameter in such analysis, although the economyVarious <i>Vi> S–SPT– <i>Ni> correlations are routinely incorporated in seismic hazardanalysis to estimate the value of <i>Vi> S.i> S calculations.The scattering nature of the <i>Vi> S values estimated using different <i>Vi> S

Keywords： uncertainties     <i>Vi> S– <i>Ni> correlations     <i>Vi> S30     SPT data     statistical    

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Anti-β glycoprotein I antibodies in complex with β2 glycoprotein I induce platelet activation via tworeceptors: apolipoprotein E receptor 2' and glycoprotein I bα

Wenjing Zhang,Fei Gao,Donghe Lu,Na Sun,Xiaoxue Yin,Meili Jin,Yanhong Liu

Frontiers of Medicine 2016, Volume 10, Issue 1,   Pages 76-84 doi: 10.1007/s11684-015-0426-7

Abstract:

Anti-β2 glycoprotein I (anti-β2GP I ) antibodies are importantba (GP I bα) in platelet activation induced by the anti-β2GP I /β22GP I /β2GP I complex and plateletsOur results revealed that the anti-β2GP I /β2GP I complex was able toResults showed that the anti-β2GP I /β2GP I complex induced platelet

Keywords： anti-β2GP I /β2GP I complex     platelet     GP I bα     apoER2'     thrombosis    

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Extracellular vesicle-carried GTF2I from mesenchymal stem cells promotes the expression of tumor-suppressive

Frontiers of Medicine doi: 10.1007/s11684-023-0999-5

Abstract: Further, Pearson’s correlation coefficient revealed a positive correlation between GTF2I expression andTherefore, we selected them for this present study, where the effects of bone marrow mesenchymal stem cell-derivedThe under-expression of GTF2I and FAT1 was validated in TC cell lines.MSC-EVs could shuttle GTF2I into TPC-1 cells, where GTF2I inhibited TC malignant phenotypes, EMT, and<i>In vivoi> experiments confirmed that silencing of GTF2I accelerated tumor growth in nude mice.

Keywords： thyroid carcinoma     mesenchymal stem cell     extracellular vesicle     GTF2I     FAT1     CDK4    

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SARS-CoV-2 Entry Factors: <i>ACE2i> and <i>TMPRSS2i> Are Expressed in Peri-Implantation Embryos and Article

Wei Chen, Peng Yuan, Ming Yang, Zhiqiang Yan, Siming Kong, Jie Yan, Xixi Liu, Yidong Chen, Jie Qiao, Liying Yan

Engineering 2020, Volume 6, Issue 10,   Pages 1162-1169 doi: 10.1016/j.eng.2020.07.013

Abstract:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the world, leading(SARS-CoV) and SARS-CoV-2.interface using previously published single-cell transcriptome data./em> and TMPRSS2, indicating SARS-CoV-2 infection susceptibility.The ACE2-positive-expressing cells in the three cell types mentioned above share common characteristics

Keywords： SARS-CoV-2     ACE2     Vertical transmission     Placenta     Peri-implantation    

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<i>RR1i> and <i>RR2i> gene deletion affects the immunogenicity of a live attenuated pseudorabies

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1,   Pages 81-86 doi: 10.15302/J-FASE-2016088

Abstract: As virulence-determining genes, and encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted and genes based on a / / triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

Keywords： pseudorabies virus     <i>RR1i> and <i>RR2i>     ribonucleotide reductase     vaccine candidate    

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Regulating surface chemistry of separator with LiF for advanced Li-S batteries

Frontiers in Energy 2022, Volume 16, Issue 4,   Pages 601-606 doi: 10.1007/s11708-021-0759-7

Abstract: Lithium-sulfur (Li-S) batteries have attracted intensive attention owing to their ultrahigh theoreticalNevertheless, the practical application of Li-S batteries is prevented by uncontrollable shuttle effectThe functional separator demonstrates a great ability to suppress active S loss and protect lithium anodeThis work provides a facile strategy for the development of advanced Li-S batteries.

Keywords： Li<i>-i>S batteries     LiF     functional separator    

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Evaluation of H2 on the Evolution Mechanism of NO<i>xi> Storage and Reductionover Pt–Ba–Ce/γ-Al2O3 Catalysts Article

Pan Wang, Jing Yi, Chuan Sun, Peng Luo, Lili Lei

Engineering 2019, Volume 5, Issue 3,   Pages 568-575 doi: 10.1016/j.eng.2019.02.005

Abstract:

In this investigation, Pt–Ba–Ce/γ-Al2O3 catalysts were preparedby incipient wetness impregnation and experiments were performed to evaluate the influence of H2 onThe physical and chemical properties of the Pt–Ba–Ce/γ-Al2O3/sub> exposure (i.e., 30 s, 45 s, and 60 s) was extended.conversion of the catalyst reached 83.5% when the duration of the lean and rich phases was 240 and 60 s,

Keywords： Pt–Ba–Ce/γ-Al2O3 catalysts     Physicochemical properties     NO<i>xi> storageand reduction     NO<i>xi> emission     H2 reductant    

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Deciphering the Origins of P1-Induced Power Losses in Cu(In<i>xi>,Ga1–<i>xi>)Se2 (CIGS) Modules Through Hyperspectral Luminescence Article

César Omar Ramírez Quiroz, Laura-Isabelle Dion-Bertrand, Christoph J. Brabec, Joachim Müller, Kay Orgassa

Engineering 2020, Volume 6, Issue 12,   Pages 1395-1402 doi: 10.1016/j.eng.2019.12.019

Abstract: patterning interconnections of subcells on Cu(Inx,Ga1-x)Se2<

Keywords： Cu(Inx     Ga1-x)Se2     Cell-to-module efficiency gap     P1-induced power losses     Hyperspectral photoluminescence    

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Enhanced production of

Shen Huang,Xudong Feng,Chun Li

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 4,   Pages 501-510 doi: 10.1007/s11705-015-1544-0

Abstract: -Glucuronidase from Li-3 (PGUS) can efficiently hydrolyze glycyrrhizin into the more valuable glycyrrhetic acid monoglucuronide. However, a low productivity of PGUS and the lack of an effective separation strategy have significantly limited its industrial applications. Therefore, the production of PGUS has been improved by optimizing both the fermentation and purification strategies. A two-stage fermentation strategy was developed where PGUS was first grown with glucose and then PGUS was produced in the presence of glycyrrhizin as an inducer. By using this strategy, the biomass was increased 1.5 times and the PGUS activity increased 5.4 times compared to that when glycyrrhizin was used as the sole carbon source. The amount of PGUS produced was increased another 16.6% when the fermentation was expanded to a 15-L fermenter. An effective protocol was also established to purify the PGUS using a sequential combination of hydrophobic, strong anion-exchange and gel filtration chromatography. This protocol had a recovery yield of 6% and gave PGUS that was 39 times purer than the crude PGUS. The purified PGUS had a specific activity of 350 U·mg .

Keywords： <i>βi>-glucuronidase     glycyrrhetic acid monoglucuronide     cell disruption     purification     chromatography    

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Title Author Date Type Operation

Structural prediction of porcine sialoadhesin V-set Ig-like domain sheds some light on its role in porcine reproductive and respiratory syndrome virus (PRRSV) infection

Jie HOU,Rui LI,Hongfang MA,Songlin QIAO,Gaiping ZHANG

Journal Article

<i>Ni>-Positive ion activated rapid addition and mitochondrial targeting ratiometric fluorescent probesfor <i>in vivoi> cell H2S imaging

Yan Shi, Fangjun Huo, Yongkang Yue, Caixia Yin

Journal Article

NO<i>xi> and H2S formation in the reductive zone of air-staged combustion of

Jinzhi CAI, Dan LI, Denggao CHEN, Zhenshan LI

Journal Article

Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis

Journal Article

Aerodynamic design by jointly applying S2 flow surface calculation and modern optimization methods on

ZHAO Honglei, WANG Songtao, HAN Wanjin, FENG Guotai

Journal Article

<i>FESEi>’s Best Papers of 2017

Journal Article

Accounting for the uncertainties in the estimation of average shear wave velocity using – correlations

Journal Article

Anti-β glycoprotein I antibodies in complex with β2 glycoprotein I induce platelet activation via tworeceptors: apolipoprotein E receptor 2' and glycoprotein I bα

Wenjing Zhang,Fei Gao,Donghe Lu,Na Sun,Xiaoxue Yin,Meili Jin,Yanhong Liu

Journal Article

Extracellular vesicle-carried GTF2I from mesenchymal stem cells promotes the expression of tumor-suppressive

Journal Article

SARS-CoV-2 Entry Factors: <i>ACE2i> and <i>TMPRSS2i> Are Expressed in Peri-Implantation Embryos and

Wei Chen, Peng Yuan, Ming Yang, Zhiqiang Yan, Siming Kong, Jie Yan, Xixi Liu, Yidong Chen, Jie Qiao, Liying Yan

Journal Article

<i>RR1i> and <i>RR2i> gene deletion affects the immunogenicity of a live attenuated pseudorabies

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

Journal Article

Regulating surface chemistry of separator with LiF for advanced Li-S batteries

Journal Article

Evaluation of H2 on the Evolution Mechanism of NO<i>xi> Storage and Reductionover Pt–Ba–Ce/γ-Al2O3 Catalysts

Pan Wang, Jing Yi, Chuan Sun, Peng Luo, Lili Lei

Journal Article

Deciphering the Origins of P1-Induced Power Losses in Cu(In<i>xi>,Ga1–<i>xi>)Se2 (CIGS) Modules Through Hyperspectral Luminescence

César Omar Ramírez Quiroz, Laura-Isabelle Dion-Bertrand, Christoph J. Brabec, Joachim Müller, Kay Orgassa

Journal Article

Enhanced production of

Shen Huang,Xudong Feng,Chun Li

Journal Article