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Prevalence and drug resistance characteristics of carbapenem-resistant Enterobacteriaceae in Hangzhou

Yan Yang, Jian Chen, Di Lin, Xujian Xu, Jun Cheng, Changgui Sun

Frontiers of Medicine 2018, Volume 12, Issue 2, Pages 182-188 doi: 10.1007/s11684-017-0529-4

Abstract: the abuse of antimicrobial agents in developing countries, increasing number of carbapenem-resistant Enterobacteriaceae

Keywords： Enterobacteriaceae carbapenem β-lactamase genes phylogenetic analysis

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Characterization of NDM-1-producing carbapenemase in

Rongmin ZHANG,Yang WANG,Zhihai LIU,Jiyun LI,Wenjuan YIN,Lei LEI,Congming WU,Jianzhong SHEN

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3, Pages 223-229 doi: 10.15302/J-FASE-2015065

Abstract: In recent years, the mobile metallo-β-lactamase (MBL) genes have been found to correspond to one of the most important resistance characters identified in Gram-negative bacteria, severely affecting clinical chemotherapy and threatening public health. The prevalence of mobile MBL genes and their flanking regions in Gram-negative bacteria from diseased pigs in China was investigated. A total of 334 lung samples from diseased pigs were screened for Gram-negative bacteria classified as non-susceptible to meropenem (MIC≥4 mg·L ). Six isolates, including three , two and one , exhibited MBL production and carried the gene. S1-PFGE and Southern blot analysis showed that the gene was located on the chromosome of one isolate and on plasmids of various sizes in the other five isolates. MIC testing using broth microdilution revealed that all -carrying isolates and some of their transconjugants exhibited resistance to almost all β-lactams tested. Whole genome sequencing revealed that the flanking region of the gene from all porcine isolates had high levels of similarity with the corresponding regions in human isolates. One porcine isolate carrying was typed as ST48, a common sequence type in human isolates. These results suggest the possibility of human-to-food animal transfer of -producing , highlighting the need for surveillance of carbapenemase producers among bacteria from food animals. In addition, the prudent use of antimicrobial agents to decrease the opportunities for co-selection of carbapenemase genes in food animals is also urgently needed.

Keywords： carbapenemase NDM-1 ISAba125 Enterobacteriaceae food safety