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Analysis of two new degradation products of arsenic triglutathione in aqueous solution

Feng ZHAO, Yuchen CHEN, Bin QIAO, Jing WANG, Ping NA

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 3,   Pages 292-300 doi: 10.1007/s11705-012-1208-2

Abstract: aqueous solution using high-performance liquid chromatography-electrospray ionisation mass spectrometry (HPLC-ESI-MSBased on the fragments obtained from MS and MS , we identified two new compounds: one was an isomer

Keywords： arsenic triglutathione     glutathione     HPLC-ESI-MS    

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Prospect in Creating New Drugs from Chinese Herbal Medicine

Yu Dequan

Strategic Study of CAE 1999, Volume 1, Issue 2,   Pages 87-90

Abstract: product research should be featured by the application of modern spectroscopic techniques, such as HPLC/MS/MS and HPLC/NMR and so on.

Keywords： research     creating new drug     chemical constituent of Chinese herbal medicine     modem spectroscopic technique     HPLC/MS/MS     HPLC/NMR    

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Photocatalytic degradation of methyl orange using ZnO/TiO composites

Ming GE , Changsheng GUO , Xingwang ZHU , Lili MA , Wei HU , Yuqiu WANG , Zhenan HAN ,

Frontiers of Environmental Science & Engineering 2009, Volume 3, Issue 3,   Pages 271-280 doi: 10.1007/s11783-009-0035-2

Abstract: ZnO/TiO composites were synthesized by using the solvothermal method and ultrasonic precipitation followed by heat treatment in order to investigate their photocatalytic degradation of methyl orange (MO) in aqueous suspension under UV irradiation. The composition and surface structure of the catalyst were characterized by X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM), and transmission electron microscopy (TEM). The degradation efficiencies of MO at various pH values were obtained. The highest degradation efficiencies were obtained before 30min and after 60min at pH 11.0 and pH 2.0, respectively. A sample analysis was conducted using liquid chromatography coupled with electrospray ionization ion-trap mass spectrometry. Six intermediates were found during the photocatalytic degradation process of quinonoid MO. The degradation pathway of quinonoid MO was also proposed.

Keywords： methyl orange     ZnO/TiO2 composites     high performance liquid chromatography mass spectrometry (HPLC-MS    

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Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis

Yansong HOU, Wei LIANG, Liping ZHANG, Shuiping CHENG, Feng HE, Zhenbin WU

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 84-91 doi: 10.1007/s11783-010-0283-1

Abstract: A high-performance liquid chromatographic (HPLC) method based on a reverse-phase C nonpolar column wasBased on the elution order using HPLC, 19 pigments were identified, and they were chlorophyllide , 19A comparison study of cell microscopic counts and accessory pigment analysis indicated that HPLC analysis

Keywords： high-performance liquid chromatographic (HPLC)     algae     pigment     chemotaxonomy    

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Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAOMS,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 216-219 doi: 10.1007/s11684-010-0037-2

Abstract: To acquire data of Brucella cellular fatty acids (CFAs) and probe into the possibility of utilizing CFAs information in typing Brucella, 19 reference strains were subjected to CFAs study. After all strains were inoculated on Brucella Agar plates, the cells were harvested, saponificated, methylated and extracted to provide fatty acid methylesters for gas chromatography (GC) analysis. Based on the CFAs data matrix, a dendrogram of 19 reference strains was generated by SPSS11.5 software package. The results showed that 19 reference strains were divided into five clusters: cluster 1 included (bv. 1, 2, 3, 5) and ; cluster 2 included (bv. 3, 4, 5, 6) and (bv. 1, 2, 3); cluster 3 included (bv. 1, 2, 7, 9) and ; cluster 4 was (bv. 4); and cluster 5 was . Typing Brucella by GC analysis of CFAs is a good method to reflect drug resistance of Brucella, and the classification is beneficial for clinical therapy. It also provides a new result of typing and demonstrates that the traditional classification is not completely reasonable. CFAs analysis may identify (bv. 4) and .

Keywords： Brucella     gas chromatography     cellular fatty acids     dendrogram     typing    

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Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

Fanghe LI BM , Chunyan ZHANG MS , Jinghua LIU MS , Xiaoyan ZHANG MS , Bing YAN , Bo ZHANG MS , YongguoHUANG MS , Jingsong GONG BM , Yan CHEN BM ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 277-283 doi: 10.1007/s11684-009-0047-0

Abstract: Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay (ELISA), a hybridoma cell line strain secreting anti-HBs monoclonal antibody (mAb) (defined G6 mAb) was obtained. The cells grew and secreted mAb stably. Antibody titers in the culture supernatant and ascites were 2.048×10 and 4.096×10, respectively. By applying the anti-HBs G6 mAb and horseradish peroxidase (HRP)-labeled goat anti-HBs antibody, we developed a sandwich ELISA (defined G6m ELISA) for detecting both wild-type and immune escape mutant HBsAgs (IEM HBsAg). The assay was performed to detect 17 species of genome recombinant expression HBsAg, including two wild-type species and 15 IEM HBsAg species, which varied in the “a” determinant, in a group of patients infected with hepatitis B virus (HBV). The patients previously had a lower ELISA detection signal [(absorbance of patients/absorbance of normal people (P/N): 1.0―4.5)]. The results demonstrated that the sensitivity of this assay to wild-type HBsAg was no less than 0.125g/L; 12 of 15 IEM HBsAg species (P/N≥2.5) were positive for G6 mAb. Of the positive IEM HBsAg species, two had a low absorbance value at 450nm (), one had an intermediate value and nine had a high value, which was 7.55%(mean), 59.4% and 92.1%―109.4% of the wild-type value, respectively. The two species with low value and the three negative species mutated at the bases 120―124 in the first loop of the HBV “a” determinant. Using the G6 ELISA and two commercial ELISA kits (A and B), 177 patients were tested. The G6 ELISA had a significantly higher detection rate than either commercial ELISAs (19.21% 14.89% and 6.21%, respectively; <0.01, <0.05, respectively).

Keywords： hepatitis B virus     gene variation     hepatitis B surface antigen     immune escape     enzyme-linked immunosorbent assay    

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Study on factors affecting TB/HIV co-infection in four counties of China

Shi-Ming CHENG MD, Yu-Ji LAI MS, Er-Yong LIU MS, Lin ZHOU MD, Xue-Jing WANG MS, Qiu-Lan CHEN MS, Dong-MingLI MS, Ning WANG MD,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 185-191 doi: 10.1007/s11684-010-0040-7

Abstract: The factors affecting case detection of tuberculosis (TB)/human immunodeficiency virus (HIV) co-infection cases were explored in order to provide evidence-based formulation of a TB/HIV co-infection control strategy in China. Four counties with different modes of HIV transmission and varying cooperation mechanisms between TB and HIV institutions were selected. HIV test among TB patients and TB examination among people living with HIV/acquired immunodeficiency syndrome (AIDS) were conducted. Patients also completed questionnaires on potential factors affecting TB/HIV co-infection. The results showed that 19 (1.7%) were HIV-positive among 1089 cases of people living with active TB who received HIV testing. Conversely, there were 126 (10%) cases of active TB among 1255 cases of people living with HIV/AIDS. Among the newly discovered 145 cases of TB/HIV co-infection, AIDS institutions found 86.9% of these co-infections and 14.1% came from TB institutes. In different registration categories, there were 31 (21.4%) cases of smear positive TB, 104 (71.7%) cases of smear negative TB, and extrapulmonary cases accounted for 10 (6.9%). Gender, occupation, marital status, ethnic group, education, HIV transmission route, CD4 level, and type of TB susceptible symptoms had significant effects on whether HIV/AIDS patients were co-infected with TB disease (<0.01). Sex, age, ethnic group, occupation, and type of tuberculosis had no significant influence on whether TB patients had HIV infection. AIDS institutions could find more TB/HIV co-infection patients in comparison with TB institutions, particularly in patients with smear negative pulmonary tuberculosis and extrapulmonary tuberculosis. Therefore, a cooperation mechanism should be built up between TB and HIV control systems, and routine TB checks should be conducted for HIV/AIDS patients. In areas where sex or drug use is the major transmission mode of HIV infection, antiretroviral therapy should be expanded, treatment adherence should be improved and patients’ immunity level should be increased in order to decrease TB incidence. Cough and expectoration of more than two weeks, bloody sputum, and recurrent fevers could be revealing symptoms in TB screening of HIV/AIDS patients.

Keywords： tuberculosis/acquired immunodeficiency syndrome     dual infection     prevention and control     case detection     impact factors    

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LC/MS/MS and radioisotope method combined for recognizing the affinity between catalpol and OCT2 transporter

Zhiyu ZHANG, Changxiao LIU, Duanyun SI, Rong LU, Xiulin YI

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 4,   Pages 436-442 doi: 10.1007/s11705-012-1222-4

Abstract: In this paper, chromatography/tandem mass spectrometry (LC/MS/MS) combined with radioisotope method wasAnd samples were precipitated with methanol and quantified with LC/MS/MS.

Keywords： catalpol     LC/MS/MS     OCT2    

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HPLC determination of glyoxal in aldehyde solution with 3-methyl-2-benzothiazolinone hydrazone

Yamei ZHU, Xiaoli YAO, Shaohui CHEN, Qun CUI, Haiyan WANG

Frontiers of Chemical Science and Engineering 2011, Volume 5, Issue 1,   Pages 117-121 doi: 10.1007/s11705-010-0535-4

Abstract: -methyl-2-benzothiazolinone hydrazone (MBTH) in the Ultraviolet-visible (UV-vis) spectral region, a HPLCGlyoxal was derivatised from MBTH and the derivatives (diazine) were analyzed by HPLC for identificationyellow dye with a maximum molar absorptivity at 401 nm and its retention time was 5.2 min under optimal HPLCThis efficient HPLC technique is also proposed for detecting other dicarbonyl compounds besides glyoxal

Keywords： HPLC     glyoxal     3-methyl-2-benzothiazolinone hydrazone     diazine     dicarbonyl compounds    

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A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host, Pomacea canaliculata

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 204-207 doi: 10.1007/s11684-010-0043-4

Abstract: Pooling field specimens could reduce the number of assay and thus increase the efficiency in detecting and screening pathogen infections by polymerase chain reaction (PCR)-based assay. We investigated a pooling strategy in diagnosis of in Two settings of specimens were prepared, divided into portions and detected by multiplex PCR. Specimens A was 0.4490 g positive lung tissue of 28 larval nodes from four snails mixed with 1.310€g negative lung tissue from six snails and divided into 32 portions. Specimens B was 0.5448 g positive lung tissue with 26 larval nodes from two snails mixed with 1.092 g negative lung tissue from seven snails and divided into 48 portions. Repeated sampling was performed and sample size-accumulated positive rate curves were drawn. According to the sample size-accumulated positive rate curves, the appropriate sample size of the two specimens were 18 and 15, respectively, which is 0.36–0.58 to the total sample size. These test characteristics and the relevant factors to the sample size would need to be determined in much larger studies and more appropriately in field populations. The result indicates that the number of larval node is not the most important, nor the only factor to the sample size. It also implies the feasibility to detect in by pooling strategies.

Keywords： Angiostrongylus cantonensis     Pomacea canaliculata     multiplex polymerase chain reaction     diagnosis     pooling    

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The Physical Precursors for the Ms 8.7 and Ms 8.5 Earthquakes in Indian Ocean

Sun Wei,Sun Xiaoming

Strategic Study of CAE 2008, Volume 10, Issue 2,   Pages 14-25

Abstract: precursors-the gestation process and the signal of impending earthquake of the Indian Ocean Earthquake of Msthat there are great differences for the stress in crust between before and after the eart hquake of Ms8-7 and that of Ms 8-5.tsunami is analyzed.The sign of underground material migration is also found in the gestation process of Ms

Keywords： Ms 8-7 and Ms 8-5 earthquake in Indian Ocean     earthquqke precursor     signal of impending earthquake     tsunami    

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The inactivation of bacteriophages MS2 and PhiX174 by nanoscale zero-valent iron: Resistance difference

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 8, doi: 10.1007/s11783-022-1529-4

Abstract:

• The resistance of phage PhiX174 to nZVI was much stronger than that of MS2

Keywords： Nanoscale zero-valent iron (nZVI)     MS2     PhiΧ174     Resistance     Inactivation     Pathogenic microorganisms    

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Mature cystic teratoma of the parotid gland: a case report and review of the literature

Lenan SHAO DMD, MD, Hanxiong GUAN MD, Jie WAN MS,

Frontiers of Medicine 2009, Volume 3, Issue 4,   Pages 503-506 doi: 10.1007/s11684-009-0076-8

Abstract: We report one case of mature cystic teratoma that presented as a tender, well-defined round mass in the right parotid gland region. Teratomas are common neoplasms but are very rarely found in the parotid gland region, so diagnosis and treatment when such occurs is a challenge. Magnetic resonance imaging scanning is useful in determining the nature of teratomas, and only surgery and pathology can provide a final diagnosis. The treatment strategy is to preserve both the facial nerve and the surrounding parotid gland tissue, while completely extirpating the teratoma.

Keywords： teratoma     parotid gland    

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Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on Persistent Organic Pollutants (2008–2019)

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 10, doi: 10.1007/s11783-022-1541-8

Abstract:

● Perfluorooctanesulfonic acid and perfluorooctanoic acid highest in human milk.

Keywords： Human biomonitoring     Human breast milk     LC-MS/MS analysis     Lifestyle parameters    

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and bacteriophage MS2 disinfection by UV, ozone and the combined UV and ozone processes

Jingyun FANG,Huiling LIU,Chii SHANG,Minzhen ZENG,Mengling NI,Wei LIU

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 4,   Pages 547-552 doi: 10.1007/s11783-013-0620-2

Abstract: In this study, inactivation of and bacteriophage MS2 by UV, ozone, UV/ozone coexposure, and sequentialThe synergistic effect on MS2 inactivation was less significant after the UV/ozone coexposure, and more

Keywords： bacteria inactivation     photoreactivation     water disinfection     UV     ozone    

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Title Author Date Type Operation

Analysis of two new degradation products of arsenic triglutathione in aqueous solution

Feng ZHAO, Yuchen CHEN, Bin QIAO, Jing WANG, Ping NA

Journal Article

Prospect in Creating New Drugs from Chinese Herbal Medicine

Yu Dequan

Journal Article

Photocatalytic degradation of methyl orange using ZnO/TiO composites

Ming GE , Changsheng GUO , Xingwang ZHU , Lili MA , Wei HU , Yuqiu WANG , Zhenan HAN ,

Journal Article

Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis

Yansong HOU, Wei LIANG, Liping ZHANG, Shuiping CHENG, Feng HE, Zhenbin WU

Journal Article

Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAOMS,

Journal Article

Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

Fanghe LI BM , Chunyan ZHANG MS , Jinghua LIU MS , Xiaoyan ZHANG MS , Bing YAN , Bo ZHANG MS , YongguoHUANG MS , Jingsong GONG BM , Yan CHEN BM ,

Journal Article

Study on factors affecting TB/HIV co-infection in four counties of China

Shi-Ming CHENG MD, Yu-Ji LAI MS, Er-Yong LIU MS, Lin ZHOU MD, Xue-Jing WANG MS, Qiu-Lan CHEN MS, Dong-MingLI MS, Ning WANG MD,

Journal Article

LC/MS/MS and radioisotope method combined for recognizing the affinity between catalpol and OCT2 transporter

Zhiyu ZHANG, Changxiao LIU, Duanyun SI, Rong LU, Xiulin YI

Journal Article

HPLC determination of glyoxal in aldehyde solution with 3-methyl-2-benzothiazolinone hydrazone

Yamei ZHU, Xiaoli YAO, Shaohui CHEN, Qun CUI, Haiyan WANG

Journal Article

A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host, Pomacea canaliculata

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

Journal Article

The Physical Precursors for the Ms 8.7 and Ms 8.5 Earthquakes in Indian Ocean

Sun Wei,Sun Xiaoming

Journal Article

The inactivation of bacteriophages MS2 and PhiX174 by nanoscale zero-valent iron: Resistance difference

Journal Article

Mature cystic teratoma of the parotid gland: a case report and review of the literature

Lenan SHAO DMD, MD, Hanxiong GUAN MD, Jie WAN MS,

Journal Article

Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on Persistent Organic Pollutants (2008–2019)

Journal Article

and bacteriophage MS2 disinfection by UV, ozone and the combined UV and ozone processes

Jingyun FANG,Huiling LIU,Chii SHANG,Minzhen ZENG,Mengling NI,Wei LIU

Journal Article