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Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering Article
Yuan Ma, Mingdong Yao, Bingzhi Li, Mingzhu Ding, Bo He, Si Chen, Xiao Zhou, Yingjin Yuan
Engineering 2018, Volume 4, Issue 6, Pages 888-893 doi: 10.1016/j.eng.2018.09.007
Poly(ethylene terephthalate) hydrolase (PETase) from Ideonella sakaiensis exhibits a strong abilityTherefore, we explored the interaction between PETase and the substrate (a dimer of the PET monomer ethyleneterephthalate, 2PET), using a model of PETase and its substrate.These PETase mutants were designed and tested.Thus, this study has created enhanced artificial PETase enzymes through the rational protein engineering
Keywords: Polyesterase PET degradation Cell-free protein synthesis Polyester PETase
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