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Colorado potato beetle (CPB) 1

Corn rootworm (CRW) 1

PEDV 1

RNA interference 1

RNA interference (RNAi) 1

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Soy stink bug (SSB) 1

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cyclin-dependent kinase 4 1

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gene silencing 1

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piglet diarrhea 1

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RNA-Based Biocontrols—A New Paradigm in Crop Protection Review

Matthew Bramlett, Geert Plaetinck, Peter Maienfisch

Engineering 2020, Volume 6, Issue 5,   Pages 522-527 doi: 10.1016/j.eng.2019.09.008

Abstract: Over the last years, the RNA interference (RNAi) process has been identified as a very promising new

Keywords： RNA-based biocontrols     RNA interference (RNAi)     Colorado potato beetle (CPB)     Corn rootworm (CRW)     Soy    

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Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene

Feng JIANG PhD , Xuezhen WANG PhD , Zheng XUE MD , Suming ZHANG PhD , Siyu FANG BM , Min ZHANG MD, PhD ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 287-291 doi: 10.1007/s11684-009-0050-5

Abstract: cyclin-dependent kinase 4 (cdk4) in neurodegenerative diseases, lentiviral-delivered RNA interference (RNAiLentivector-mediated RNAi could efficiently down-regulate the expression of the murine cdk4 gene , which

Keywords： cyclin-dependent kinase 4     RNA interference     plasmid     lentiviral vector    

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Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4,   Pages 663-675 doi: 10.1007/s11705-017-1623-5

Abstract: This short review presents a brief introduction to RNAi therapeutics, the obstacles to their delivery

Keywords： gene therapy     RNAi therapeutics     dendrimer     nanovectors     gene silencing    

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Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine embryos

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 66-72 doi: 10.15302/J-FASE-2018229

Abstract:

Porcine viral diarrhea is an acute and highly contagious enteric disease of pigs that causes huge economic losses worldwide. Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are the main pathogens responsible for piglet viral diarrhea. However, currently there is no specific drug available for the effective treatment of viral diarrhea. Therefore, it is necessary to seek an effective method to diminish PEDV and TGEV infection rates. RNA interference has been applied successfully to inhibit the virus replication. It provides a potential strategy for breeding resistant pigs. In this study, four promoters and four short hairpin RNA (shRNA) vectors with LoxP sites at each end of the selectable marker genes were constructed to target PEDV and TGEV. These vectors were then transfected into porcine fetal fibroblasts, G418 resistant transfectants were confirmed by PCR and transgenic SCNT porcine blastocysts were obtained. These results have paved the way for future production of marker-free transgenic resistant to PEDV and TEGV pigs by SCNT.

Keywords： piglet diarrhea     RNAi     PEDV     TGEV     transgenic resistance breeding    

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Title Author Date Type Operation

RNA-Based Biocontrols—A New Paradigm in Crop Protection

Matthew Bramlett, Geert Plaetinck, Peter Maienfisch

Journal Article

Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene

Feng JIANG PhD , Xuezhen WANG PhD , Zheng XUE MD , Suming ZHANG PhD , Siyu FANG BM , Min ZHANG MD, PhD ,

Journal Article

Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

Journal Article

Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine embryos

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Journal Article