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Production of a polyclonal antibody to the VP26 nucleocapsid protein of white spot syndrome virus (wssv

Suchera LOYPRASERT-THANANIMIT, Akrapon SALEEDANG, Proespichaya KANATHARANA, Panote THAVARUNGKUL, Wilaiwan CHOTIGEAT

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 2, Pages 216-223 doi: 10.1007/s11705-012-1289-y

Abstract: VP26 is one of the structural proteins of WSSV that is assumed to assist in recognizing its host andThe objective of this work was to produce a polyclonal antibody against VP26 and use it as a biosensorThe recombinant VP26 protein (rVP26) was produced in (BL21), purified and used for immunizing rabbitsWestern blot analysis confirmed that the antiserum had a specific immunoreactivity to the VP26 of WSSVThe impedance change in the presence of VP26 was monitored in real time.

Keywords： recombinant protein polyclonal antibody label-free biosensor impedance white spot syndrome virus (WSSV)