Rapid Authentication of the Poisonous Plant Gelsemium elegans by Combining Filter-Paper-Based DNA Extraction and RPA-LFD Detection

Xiasheng Zheng; Wenli An; Hui Yao; Jiang Xu; Shilin Chen

doi:10.1016/j.eng.2020.02.012

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Engineering ›› 2021, Vol. 7 ›› Issue (1) : 14-16. DOI: 10.1016/j.eng.2020.02.012

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Rapid Authentication of the Poisonous Plant Gelsemium elegans by Combining Filter-Paper-Based DNA Extraction and RPA-LFD Detection

Xiasheng Zheng^a^,^b^,^* ,
Wenli An^a^,^* ,
Hui Yao^c ,
Jiang Xu^b ,
Shilin Chen^b

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Xiasheng Zheng, Wenli An, Hui Yao, Jiang Xu, Shilin Chen. Rapid Authentication of the Poisonous Plant Gelsemium elegans by Combining Filter-Paper-Based DNA Extraction and RPA-LFD Detection. Engineering, 2021, 7(1): 14‒16 https://doi.org/10.1016/j.eng.2020.02.012

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[2]	Zou Y, Mason MG, Wang Y, Wee E, Turni C, Blackall PJ, et al. Nucleic acid purification from plants, animals and microbes in under 30 seconds. PLoS Biol 2017;15(11):e2003916.

[3]	Liu YC, Lin L, Cheng P, Sun ZL, Wu Y, Liu ZY. Fingerprint analysis of Gelsemium elegans by HPLC followed by the targeted identification of chemical constituents using HPLC coupled with quadrupole-time-of-flight mass spectrometry. Fitoterapia 2017;121:94–105.