Temporal Dynamics and Performance Association of the <i>Tetrasphaera-</i>Enriched Microbiome for Enhanced Biological Phosphorus Removal

Hui Wang; Yubo Wang; Guoqing Zhang; Ze Zhao; Feng Ju

doi:10.1016/j.eng.2022.10.016

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Engineering ›› 2023, Vol. 29 ›› Issue (10) : 168-178. DOI: 10.1016/j.eng.2022.10.016

Research

Article

Temporal Dynamics and Performance Association of the Tetrasphaera-Enriched Microbiome for Enhanced Biological Phosphorus Removal

Hui Wang^a^,^b^,^c ,
Yubo Wang^b^,^c ,
Guoqing Zhang^b ,
Ze Zhao^b ,
Feng Ju^b^,^c^,^d^,^e^,^*

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Highlights

• A novel putative Tetrasphaera PAOs species (EBPR-ASV0001) was enriched.

• Short-term bio-stimulation with 1 mg⋅L^-1 allylthiourea promoted Tetrasphaera-enriched microbiome establishment.

• Microlunatus participated the Tetrasphaera-enriched microbiome establishment and function.

• Tetrasphaera-enriched microbiome showed high potential in P resource recovery.

Abstract

Tetrasphaera have been recently identified based on the 16S ribosomal RNA (rRNA) gene as among the most abundant polyphosphate-accumulating organisms (PAOs) in global full-scale wastewater treatment plants (WWTPs) with enhanced biological phosphorus removal (EBPR). However, it is unclear how Tetrasphaera PAOs are selectively enriched in the context of the EBPR microbiome. In this study, an EBPR microbiome enriched with Tetrasphaera (accounting for 40% of 16S sequences on day 113) was built using a top-down design approach featuring multicarbon sources and a low dosage of allylthiourea. The microbiome showed enhanced nutrient removal (phosphorus removal ∼85% and nitrogen removal ∼80%) and increased phosphorus recovery (up to 23.2 times) compared with the seeding activated sludge from a local full-scale WWTP. The supply of 1 mg·L⁻¹ allylthiourea promoted the coselection of Tetrasphaera PAOs and Microlunatus PAOs and sharply reduced the relative abundance of both ammonia oxidizer Nitrosomonas and putative competitors Brevundimonas and Paracoccus, facilitating the establishment of the EBPR microbiome. Based on 16S rRNA gene analysis, a putative novel PAO species, EBPR-ASV0001, was identified with Tetrasphaera japonica as its closest relative. This study provides new knowledge on the establishment of a Tetrasphaera-enriched microbiome facilitated by allylthiourea, which can be further exploited to guide future process upgrading and optimization to achieve and/or enhance simultaneous biological phosphorus and nitrogen removal from high-strength wastewater.

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Keywords

Enhanced biological phosphorus removal (EBPR) / Polyphosphate-accumulating organisms (PAOs) / Tetrasphaera / Microbiome / Phosphorus recovery

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Hui Wang, Yubo Wang, Guoqing Zhang, Ze Zhao, Feng Ju. Temporal Dynamics and Performance Association of the Tetrasphaera-Enriched Microbiome for Enhanced Biological Phosphorus Removal. Engineering, 2023, 29(10): 168‒178 https://doi.org/10.1016/j.eng.2022.10.016

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This work was supported by the Key Research and Development Program of Zhejiang (2022C03075), National Natural Science Foundation of China (22241603), and Zhejiang Provincial Natural Science Foundation of China (LR22D010001). We would like to thank Dr. Xiao Yang and Dr. Xiangyu Yang for the helpful discussion and technical advice. The author would like to thank Yisong Xu for her professional support in equipment procurement and lab management. We thank the Microscopy Core Facility of Westlake University for the facility support and thank technician Fang Xiao for technical assistance. We thank the Research Center for Industries of the Future (RCIF), the Instrumentation and Service Center for Molecular Sciences and Physical Sciences, and The Westlake University-Muyuan Group Joint Research Institute at Westlake University for support. We thank the Westlake University High-Performance Computing Center for computation support.

Funding

the Key Research and Development Program of Zhejiang(2022C03075); National Natural Science Foundation of China(22241603); Zhejiang Provincial Natural Science Foundation of China(LR22D010001)