The transplantation of full-thickness skin grafts (FTSGs) is important for reconstructing skin barrier and promoting wound healing. Sufficient oxygen supply is closely related to the success of skin grafting. However, full-thickness oxygen delivery is limited by the poor oxygen permeability of skin. Oxygen-releasing sutures (O₂ sutures) were developed to facilitate oxygen penetration through full-thickness skin. The O₂ sutures delivered 100 times more oxygen than topical gaseous oxygen therapy at a 15 mm depth in the skin model. Under extreme hypoxia (< 0.5% O₂, v/v), O₂ sutures could also promote endothelial cell proliferation. After the transplantation of FTSGs in mice, O₂ sutures accelerated blood re-perfusion and increased the survival area of the skin graft. It is expected that O₂ sutures will be adopted in clinical applications to increase the success rate of full-thickness skin transplantation.